Development, Vol 127, Issue 2 413-424, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Lbx1 is required for muscle precursor migration along a lateral pathway into the limb

MK Gross, L Moran-Rivard, T Velasquez, MN Nakatsu, K Jagla and M Goulding
Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, CA 92037, USA.

In mammalian embryos, myogenic precursor cells emigrate from the ventral lip of the dermomyotome and colonize the limbs, tongue and diaphragm where they differentiate and form skeletal muscle. Previous studies have shown that Pax3, together with the c-Met receptor tyrosine kinase and its ligand Scatter Factor (SF) are necessary for the migration of hypaxial muscle precursors in mice. Lbx1 and Pax3 are co-expressed in all migrating hypaxial muscle precursors, raising the possibility that Lbx1 regulates their migration. To examine the function of Lbx1 in muscle development, we inactivated the Lbx1 gene by homologous recombination. Mice lacking Lbx1 exhibit an extensive loss of limb muscles, although some forelimb and hindlimb muscles are still present. The pattern of muscle loss suggests that Lbx1 is not required for the specification of particular limb muscles, and the muscle defects that occur in Lbx1(-/-) mice can be solely attributed to changes in muscle precursor migration. c-Met is expressed in Lbx1 mutant mice and limb muscle precursors delaminate from the ventral dermomyotome but fail to migrate laterally into the limb. Muscle precursors still migrate ventrally and give rise to tongue, diaphragm and some limb muscles, demonstrating Lbx1 is necessary for the lateral, but not ventral, migration of hypaxial muscle precursors. These results suggest that Lbx1 regulates responsiveness to a lateral migration signal which emanates from the developing limb.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. Yu, G. A. Smolen, J. Zhang, B. Wittner, B. J. Schott, E. Brachtel, S. Ramaswamy, S. Maheswaran, and D. A. Haber A developmentally regulated inducer of EMT, LBX1, contributes to breast cancer progression Genes & Dev., August 1, 2009; 23(15): 1737 - 1742. [Abstract] [Full Text] [PDF]

				D. Merrick, L. K. J. Stadler, D. Larner, and J. Smith Muscular dystrophy begins early in embryonic development deriving from stem cell loss and disrupted skeletal muscle formation Dis. Model. Mech., July 1, 2009; 2(7-8): 374 - 388. [Abstract] [Full Text] [PDF]

				G. Junion, L. Bataille, T. Jagla, J. P. Da Ponte, R. Tapin, and K. Jagla Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors Genes & Dev., December 1, 2007; 21(23): 3163 - 3180. [Abstract] [Full Text] [PDF]

				S. Watanabe, S. Kondo, M. Hayasaka, and K. Hanaoka Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle J. Cell Sci., December 1, 2007; 120(23): 4178 - 4187. [Abstract] [Full Text] [PDF]

				V. Odemis, K. Boosmann, M. T. Dieterlen, and J. Engele The chemokine SDF1 controls multiple steps of myogenesis through atypical PKC{zeta} J. Cell Sci., November 15, 2007; 120(22): 4050 - 4059. [Abstract] [Full Text] [PDF]

				S. Tozer, M.-A. Bonnin, F. Relaix, S. Di Savino, P. Garcia-Villalba, P. Coumailleau, and D. Duprez Involvement of vessels and PDGFB in muscle splitting during chick limb development Development, July 15, 2007; 134(14): 2579 - 2591. [Abstract] [Full Text] [PDF]

				H. P. Shih, M. K. Gross, and C. Kioussi Cranial muscle defects of Pitx2 mutants result from specification defects in the first branchial arch PNAS, April 3, 2007; 104(14): 5907 - 5912. [Abstract] [Full Text] [PDF]

				C. Kioussi, H.-P. Shih, J. Loflin, and M. K. Gross Prediction of active nodes in the transcriptional network of neural tube patterning PNAS, December 5, 2006; 103(49): 18621 - 18626. [Abstract] [Full Text] [PDF]

				L. Bajard, F. Relaix, M. Lagha, D. Rocancourt, P. Daubas, and M. E. Buckingham A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb Genes & Dev., September 1, 2006; 20(17): 2450 - 2464. [Abstract] [Full Text] [PDF]

				X. Shi and D. J. Garry Muscle stem cells in development, regeneration, and disease. Genes & Dev., July 1, 2006; 20(13): 1692 - 1708. [Abstract] [Full Text] [PDF]

				J. D. Porter, S. Israel, B. Gong, A. P. Merriam, J. Feuerman, S. Khanna, and H. J. Kaminski Distinctive morphological and gene/protein expression signatures during myogenesis in novel cell lines from extraocular and hindlimb muscle Physiol Genomics, February 23, 2006; 24(3): 264 - 275. [Abstract] [Full Text] [PDF]

				B. L. Martin and R. M. Harland A novel role for lbx1 in Xenopus hypaxial myogenesis Development, January 15, 2006; 133(2): 195 - 208. [Abstract] [Full Text] [PDF]

				F. Relaix, D. Montarras, S. Zaffran, B. Gayraud-Morel, D. Rocancourt, S. Tajbakhsh, A. Mansouri, A. Cumano, and M. Buckingham Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells J. Cell Biol., January 3, 2006; 172(1): 91 - 102. [Abstract] [Full Text] [PDF]

				E. Vasyutina, J. Stebler, B. Brand-Saberi, S. Schulz, E. Raz, and C. Birchmeier CXCR4 and Gab1 cooperate to control the development of migrating muscle progenitor cells Genes & Dev., September 15, 2005; 19(18): 2187 - 2198. [Abstract] [Full Text] [PDF]

				A. McDermott, M. Gustafsson, T. Elsam, C.-C. Hui, C. P. Emerson Jr, and A.-G. Borycki Gli2 and Gli3 have redundant and context-dependent function in skeletal muscle formation Development, January 15, 2005; 132(2): 345 - 357. [Abstract] [Full Text] [PDF]

				C. T. Lee, L. Li, N. Takamoto, J. F. Martin, F. J. DeMayo, M.-J. Tsai, and S. Y. Tsai The Nuclear Orphan Receptor COUP-TFII Is Required for Limb and Skeletal Muscle Development Mol. Cell. Biol., December 15, 2004; 24(24): 10835 - 10843. [Abstract] [Full Text] [PDF]

				X. Borue and D. M. Noden Normal and aberrant craniofacial myogenesis by grafted trunk somitic and segmental plate mesoderm Development, August 15, 2004; 131(16): 3967 - 3980. [Abstract] [Full Text] [PDF]

				M.-C. Delfini and D. Duprez Ectopic Myf5 or MyoD prevents the neuronal differentiation program in addition to inducing skeletal muscle differentiation, in the chick neural tube Development, February 15, 2004; 131(4): 713 - 723. [Abstract] [Full Text] [PDF]

				M. Zikova, J.-P. Da Ponte, B. Dastugue, and K. Jagla Patterning of the cardiac outflow region in Drosophila PNAS, October 14, 2003; 100(21): 12189 - 12194. [Abstract] [Full Text] [PDF]

				X. J. de Mollerat, F. Gurrieri, C. T. Morgan, E. Sangiorgi, D. B. Everman, P. Gaspari, J. Amiel, M. J. Bamshad, R. Lyle, J.-L. Blouin, et al. A genomic rearrangement resulting in a tandem duplication is associated with split hand-split foot malformation 3 (SHFM3) at 10q24 Hum. Mol. Genet., August 15, 2003; 12(16): 1959 - 1971. [Abstract] [Full Text] [PDF]

				J. Hadchouel, J. J. Carvajal, P. Daubas, L. Bajard, T. Chang, D. Rocancourt, D. Cox, D. Summerbell, S. Tajbakhsh, P. W. J. Rigby, et al. Analysis of a key regulatory region upstream of the Myf5 gene reveals multiple phases of myogenesis, orchestrated at each site by a combination of elements dispersed throughout the locus Development, August 1, 2003; 130(15): 3415 - 3426. [Abstract] [Full Text] [PDF]

				C. Laclef, G. Hamard, J. Demignon, E. Souil, C. Houbron, and P. Maire Altered myogenesis in Six1-deficient mice Development, May 15, 2003; 130(10): 2239 - 2252. [Abstract] [Full Text] [PDF]

				C. KIOUSSI, P. BRIATA, S.H. BAEK, A. WYNSHAW-BORIS, D.W. ROSE, and M.G. ROSENFELD Pitx Genes during Cardiovascular Development Cold Spring Harb Symp Quant Biol, January 1, 2002; 67(0): 81 - 88. [Abstract] [PDF]

				T. J. Hawke and D. J. Garry Myogenic satellite cells: physiology to molecular biology J Appl Physiol, August 1, 2001; 91(2): 534 - 551. [Abstract] [Full Text] [PDF]