|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 21 4619-4630, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
L Jones, H Richardson and R Saint
Centre for the Molecular Genetics of Development and Department of Molecular Biosciences, University of Adelaide, South Australia, Australia.
Cyclin E is an essential regulator of S phase entry. We have previously shown that transcriptional regulation of the gene that encodes Drosophila cyclin E, DmcycE, plays an important role in the control of the G(1) to S phase transition during development. We report here the first comprehensive analysis of the transcriptional regulation of a G(1 )phase cell cycle regulatory gene during embryogenesis. Analysis of deficiencies, a genomic transformant and reporter gene constructs revealed that DmcycE transcription is controlled by a large and complex cis-regulatory region containing tissue- and stage-specific components. Separate regulatory elements for transcription in epidermal cells during cell cycles 14-16, central nervous system cells and peripheral nervous system cells were found. An additional cis-regulatory element drives transcription in thoracic epidermal cells that undergo a 17th cell cycle when other epidermal cells have arrested in G(1 )phase prior to terminal differentiation. The complexity of DmcycE transcriptional regulation argues against a model in which DmcycE transcription is regulated simply and solely by G(1) to S phase transcription regulators such as RB, E2F and DP. Rather, our study demonstrates that tissue-specific transcriptional regulatory mechanisms are important components of the control of cyclin E transcription and thus of cell proliferation in metazoans.
This article has been cited by other articles:
|
|
 |
|
  N. Zielke, S. Querings, C. Rottig, C. Lehner, and F. Sprenger The anaphase-promoting complex/cyclosome (APC/C) is required for rereplication control in endoreplication cycles Genes & Dev., June 15, 2008; 22(12): 1690 - 1703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shibutani, L. M. Swanhart, and R. J. Duronio Rbf1-independent termination of E2f1-target gene expression during early Drosophila embryogenesis Development, February 1, 2007; 134(3): 467 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Richard-Parpaillon, R. A. Cosgrove, C. Devine, A. E. Vernon, and A. Philpott G1/S phase cyclin-dependent kinase overexpression perturbs early development and delays tissue-specific differentiation in Xenopus Development, June 1, 2004; 131(11): 2577 - 2586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Ohlmeyer and T. Schupbach Encore facilitates SCF-Ubiquitin-proteasome-dependent proteolysis during Drosophila oogenesis Development, December 22, 2003; 130(25): 6339 - 6349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kango-Singh, R. Nolo, C. Tao, P. Verstreken, P. R. Hiesinger, H. J. Bellen, and G. Halder Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila Development, March 14, 2003; 129(24): 5719 - 5730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Xin, L. Weng, J. Xu, and W. Du The role of RBF in developmentally regulated cell proliferation in the eye disc and in Cyclin D/Cdk4 induced cellular growth Development, March 5, 2003; 129(6): 1345 - 1356. [Abstract] [Full Text] [PDF]
|
|
