Development, Vol 127, Issue 24 5541-5550, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Windbeutel is required for function and correct subcellular localization of the Drosophila patterning protein Pipe

J Sen, JS Goltz, M Konsolaki, T Schupbach and D Stein
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Drosophila embryonic dorsal-ventral polarity originates in the ovarian follicle through the restriction of pipe gene expression to a ventral subpopulation of follicle cells. Pipe, a homolog of vertebrate glycosaminoglycan-modifying enzymes, directs the ventral activation of an extracellular serine proteolytic cascade which defines the ventral side of the embryo. When pipe is expressed uniformly in the follicle cell layer, a strong ventralization of the resulting embryos is observed. Here, we show that this ventralization is dependent on the other members of the dorsal group of genes controlling dorsal-ventral polarity, but not on the state of the Epidermal Growth Factor Receptor signal transduction pathway which defines egg chamber polarity. Pipe protein expressed in vertebrate tissue culture cells localizes to the endoplasmic reticulum. Strikingly, coexpression of the dorsal group gene windbeutel in those cells directs Pipe to the Golgi. Similarly, Pipe protein exhibits an altered subcellular localization in the follicle cells of females mutant for windbeutel. Thus, Windbeutel protein enables the correct subcellular distribution of Pipe to facilitate its pattern-forming activity.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				X. Zhu, L. M. Stevens, and D. Stein Synthesis of the sulfate donor PAPS in either the Drosophila germline or somatic follicle cells can support embryonic dorsal-ventral axis formation Development, April 15, 2007; 134(8): 1465 - 1469. [Abstract] [Full Text] [PDF]

				U. Lippert, D. Diao, N. N. Barak, and D. M. Ferrari Conserved Structural and Functional Properties of D-domain Containing Redox-active and -inactive Protein Disulfide Isomerase-related Protein Chaperones J. Biol. Chem., April 13, 2007; 282(15): 11213 - 11220. [Abstract] [Full Text] [PDF]

				D. Xu, D. Song, L. C. Pedersen, and J. Liu Mutational Study of Heparan Sulfate 2-O-Sulfotransferase and Chondroitin Sulfate 2-O-Sulfotransferase J. Biol. Chem., March 16, 2007; 282(11): 8356 - 8367. [Abstract] [Full Text] [PDF]

				C.-M. Cruciat, C. Hassler, and C. Niehrs The MRH Protein Erlectin Is a Member of the Endoplasmic Reticulum Synexpression Group and Functions in N-Glycan Recognition J. Biol. Chem., May 5, 2006; 281(18): 12986 - 12993. [Abstract] [Full Text] [PDF]

				X. Zhu, J. Sen, L. Stevens, J. S. Goltz, and D. Stein Drosophila Pipe protein activity in the ovary and the embryonic salivary gland does not require heparan sulfate glycosaminoglycans Development, September 1, 2005; 132(17): 3813 - 3822. [Abstract] [Full Text] [PDF]

				V. M. Hermann, J. F. Cutfield, and M. J. Hubbard Biophysical Characterization of ERp29: EVIDENCE FOR A KEY STRUCTURAL ROLE OF CYSTEINE 125 J. Biol. Chem., April 8, 2005; 280(14): 13529 - 13537. [Abstract] [Full Text] [PDF]

				K. Barnewitz, C. Guo, M. Sevvana, Q. Ma, G. M. Sheldrick, H.-D. Soling, and D. M. Ferrari Mapping of a Substrate Binding Site in the Protein Disulfide Isomerase-related Chaperone Wind Based on Protein Function and Crystal Structure J. Biol. Chem., September 17, 2004; 279(38): 39829 - 39837. [Abstract] [Full Text] [PDF]

				K. Nogami, H. Suzuki, H. Habuchi, N. Ishiguro, H. Iwata, and K. Kimata Distinctive Expression Patterns of Heparan Sulfate O-Sulfotransferases and Regional Differences in Heparan Sulfate Structure in Chick Limb Buds J. Biol. Chem., February 27, 2004; 279(9): 8219 - 8229. [Abstract] [Full Text] [PDF]

				Q. Ma, C. Guo, K. Barnewitz, G. M. Sheldrick, H.-D. Soling, I. Uson, and D. M. Ferrari Crystal Structure and Functional Analysis of Drosophila Wind, a Protein-disulfide Isomerase-related Protein J. Biol. Chem., November 7, 2003; 278(45): 44600 - 44607. [Abstract] [Full Text] [PDF]

				A. J. Chang and D. Morisato Regulation of Easter activity is required for shaping the Dorsal gradient in the Drosophila embryo Development, March 14, 2003; 129(24): 5635 - 5645. [Abstract] [Full Text] [PDF]

				F. Peri, M. Technau, and S. Roth Mechanisms of Gurken-dependent pipe regulation and the robustness of dorsoventral patterning in Drosophila Development, March 8, 2003; 129(12): 2965 - 2975. [Abstract] [Full Text] [PDF]

				K. Hoshijima, J. E. Metherall, and D. J. Grunwald A protein disulfide isomerase expressed in the embryonic midline is required for left/right asymmetries Genes & Dev., October 1, 2002; 16(19): 2518 - 2529. [Abstract] [Full Text] [PDF]

				E. Sargsyan, M. Baryshev, L. Szekely, A. Sharipo, and S. Mkrtchian Identification of ERp29, an Endoplasmic Reticulum Lumenal Protein, as a New Member of the Thyroglobulin Folding Complex J. Biol. Chem., May 3, 2002; 277(19): 17009 - 17015. [Abstract] [Full Text] [PDF]

				R. Tsruya, A. Schlesinger, A. Reich, L. Gabay, A. Sapir, and B.-Z. Shilo Intracellular trafficking by Star regulates cleavage of the Drosophila EGF receptor ligand Spitz Genes & Dev., January 15, 2002; 16(2): 222 - 234. [Abstract] [Full Text] [PDF]

				K. E. James, J. B. Dorman, and C. A. Berg Mosaic analyses reveal the function of Drosophila Ras in embryonic dorsoventral patterning and dorsal follicle cell morphogenesis Development, January 5, 2002; 129(9): 2209 - 2222. [Abstract] [Full Text] [PDF]

				D. J. Goff, L. A. Nilson, and D. Morisato Establishment of dorsal-ventral polarity of the Drosophila egg requires capicua action in ovarian follicle cells Development, November 15, 2001; 128(22): 4553 - 4562. [Abstract] [Full Text] [PDF]

				S. E. Basham and L. S. Rose The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related protein of the AAA ATPase superfamily Development, November 15, 2001; 128(22): 4645 - 4656. [Abstract] [Full Text] [PDF]