|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 5 945-956, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
JM Collinson, RE Hill and JD West
Genes and Development Group, Department of Reproductive and Developmental Sciences, University of Edinburgh, Anatomy Building, Medical School, Teviot Place, Edinburgh EH8 9AG, Scotland, UK.
Chimaeric mice were made by aggregating Pax6(-/-) and wild-type mouse embryos, in order to study the interaction between the optic vesicle and the prospective lens epithelium during early stages of eye development. Histological analysis of the distribution of homozygous mutant cells in the chimaeras showed that the cell-autonomous removal of Pax6(-/-) cells from the lens, shown previously at E12.5, is nearly complete by E9.5. Most mutant cells are eliminated from an area of facial epithelium wider than, but including, the developing lens placode. This result suggests a role for Pax6 in maintaining a region of the facial epithelium that has the tissue competence to undergo lens differentiation. Segregation of wild-type and Pax6(-/-) cells occurs in the optic vesicle at E9.5 and is most likely a result of different adhesive properties of wild-type and mutant cells. Also, proximo-distal specification of the optic vesicle (as assayed by the elimination of Pax6(-/-) cells distally), is disrupted in the presence of a high proportion of mutant cells. This suggests that Pax6 operates during the establishment of patterning along the proximo-distal axis of the vesicle. Examination of chimaeras with a high proportion of mutant cells showed that Pax6 is required in the optic vesicle for maintenance of contact with the overlying lens epithelium. This may explain why Pax6(-/-) optic vesicles are inefficient at inducing a lens placode. Contact is preferentially maintained when the lens epithelium is also wild-type. Together, these results demonstrate requirements for functional Pax6 in both the optic vesicle and surface epithelia in order to mediate the interactions between the two tissues during the earliest stages of eye development.
This article has been cited by other articles:
|
|
 |
|
  J. Favor, C. J. Gloeckner, A. Neuhauser-Klaus, W. Pretsch, R. Sandulache, S. Saule, and I. Zaus Relationship of Pax6 Activity Levels to the Extent of Eye Development in the Mouse, Mus musculus Genetics, July 1, 2008; 179(3): 1345 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Li, D. Goldowitz, and D. J. Swanson The Requirement of Pax6 for Postnatal Eye Development: Evidence from Experimental Mouse Chimeras Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3292 - 3300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Song, K. R. Schwab, L. T. Patterson, T. Yamaguchi, X. Lin, S. S. Potter, and R. A. Lang pygopus 2 has a crucial, Wnt pathway-independent function in lens induction Development, May 15, 2007; 134(10): 1873 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kucerova, J. Ou, D. Lawson, L. J. Leiper, and J. M. Collinson Cell Surface Glycoconjugate Abnormalities and Corneal Epithelial Wound Healing in the Pax6+/- Mouse Model of Aniridia-Related Keratopathy Invest. Ophthalmol. Vis. Sci., December 1, 2006; 47(12): 5276 - 5282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Gotoh, M. Ito, S. Yamamoto, I. Yoshino, N. Song, Y. Wang, I. Lax, J. Schlessinger, M. Shibuya, and R. A. Lang Tyrosine phosphorylation sites on FRS2{alpha} responsible for Shp2 recruitment are critical for induction of lens and retina PNAS, December 7, 2004; 101(49): 17144 - 17149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Collinson, S. A. Chanas, R. E. Hill, and J. D. West Corneal Development, Limbal Stem Cell Function, and Corneal Epithelial Cell Migration in the Pax6+/- Mouse Invest. Ophthalmol. Vis. Sci., April 1, 2004; 45(4): 1101 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Davis, M. K. Duncan, W. G. Robison Jr, and J. Piatigorsky Requirement for Pax6 in corneal morphogenesis: a role in adhesion J. Cell Sci., June 1, 2003; 116(11): 2157 - 2167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Yamada, Y. Mizutani-Koseki, T. Hasegawa, N. Osumi, H. Koseki, and N. Takahashi Cell-autonomous involvement of Mab21l1 is essential for lens placode development Development, May 1, 2003; 130(9): 1759 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Pratt, J. C. Quinn, T. I. Simpson, J. D. West, J. O. Mason, and D. J. Price Disruption of Early Events in Thalamocortical Tract Formation in Mice Lacking the Transcription Factors Pax6 or Foxg1 J. Neurosci., October 1, 2002; 22(19): 8523 - 8531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, A. Friedman, S. Heaney, P. Purcell, and R. L. Maas Meis homeoproteins directly regulate Pax6 during vertebrate lens morphogenesis Genes & Dev., August 15, 2002; 16(16): 2097 - 2107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. van Heyningen and K. A. Williamson PAX6 in sensory development Hum. Mol. Genet., May 15, 2002; 11(10): 1161 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Dimanlig, S. C. Faber, W. Auerbach, H. P. Makarenkova, and R. A. Lang The upstream ectoderm enhancer in Pax6 has an important role in lens induction Development, November 15, 2001; 128(22): 4415 - 4424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bernier, W. Vukovich, L. Neidhardt, B. G. Herrmann, and P. Gruss Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium Development, October 15, 2001; 128(20): 3987 - 3994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Collinson, J. C. Quinn, M. A. Buchanan, M. H. Kaufman, S. E. Wedden, J. D. West, and R. E. Hill Primary defects in the lens underlie complex anterior segment abnormalities of the Pax6 heterozygous eye PNAS, July 24, 2001; (2001) 161144098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kamachi, M. Uchikawa, A. Tanouchi, R. Sekido, and H. Kondoh Pax6 and SOX2 form a co-DNA-binding partner complex that regulates initiation of lens development Genes & Dev., May 15, 2001; 15(10): 1272 - 1286. [Abstract] [Full Text]
|
|
|
|
|
|
R. Ashery-Padan, T. Marquardt, X. Zhou, and P. Gruss Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye Genes & Dev., November 1, 2000; 14(21): 2701 - 2711. [Abstract] [Full Text]
|
|
|
|
|
|
T Pratt, T Vitalis, N Warren, J. Edgar, J. Mason, and D. Price A role for Pax6 in the normal development of dorsal thalamus and its cortical connections Development, January 12, 2000; 127(23): 5167 - 5178. [Abstract] [PDF]
|
|
|
|
|
|
C. van Raamsdonk and S. Tilghman Dosage requirement and allelic expression of PAX6 during lens placode formation Development, January 12, 2000; 127(24): 5439 - 5448. [Abstract] [PDF]
|
|
|
|
|
|
A Noveen, A Daniel, and V Hartenstein Early development of the Drosophila mushroom body: the roles of eyeless and dachshund Development, January 8, 2000; 127(16): 3475 - 3488. [Abstract] [PDF]
|
|
|
|
|
|
J. M. Collinson, J. C. Quinn, M. A. Buchanan, M. H. Kaufman, S. E. Wedden, J. D. West, and R. E. Hill Primary defects in the lens underlie complex anterior segment abnormalities of the Pax6 heterozygous eye PNAS, August 14, 2001; 98(17): 9688 - 9693. [Abstract] [Full Text] [PDF]
|
|
