spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Busturia, A.
Right arrow Articles by Sakonju, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Busturia, A.
Right arrow Articles by Sakonju, S.
Development 128, 2163-2173 (2001)
© 2001 The Company of Biologists Limited

The MCP silencer of the Drosophila Abd-B gene requires both Pleiohomeotic and GAGA factor for the maintenance of repression

Ana Busturia1, Alan Lloyd2, Fernando Bejarano1, Michael Zavortink2, Hua Xin2 and Shigeru Sakonju2,*

1 Centro de Biología Molecular, Universidad Autónoma de Madrid, CSIC-UAM, Campus de Cantoblanco, Madrid 28049, Spain
2 Department of Human Genetics, 5200 Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA

*Author for correspondence (e-mail: sakonju{at}genetics.utah.edu)

Accepted March 23, 2001

Silencing of homeotic gene expression requires the function of cis-regulatory elements known as Polycomb Response Elements (PREs). The MCP silencer element of the Drosophila homeotic gene Abdominal-B has been shown to behave as a PRE and to be required for silencing throughout development. Using deletion analysis and reporter gene assays, we defined a 138 bp sequence within the MCP silencer that is sufficient for silencing of a reporter gene in the imaginal discs. Within the MCP138 fragment, there are four binding sites for the Pleiohomeotic protein (PHO) and two binding sites for the GAGA factor (GAF), encoded by the Trithorax-like gene. PHO and the GAF proteins bind to these sites in vitro. Mutational analysis of PHO and GAF binding sequences indicate that these sites are necessary for silencing in vivo. Moreover, silencing by MCP138 depends on the function of the Trithorax-like gene, and on the function of the PcG genes, including pleiohomeotic. Deletion and mutational analyses show that, individually, either PHO or GAF binding sites retain only weak silencing activity. However, when both PHO and GAF binding sites are present, they achieve strong silencing. We present a model in which robust silencing is achieved by sequential and facilitated binding of PHO and GAF.

Key words: MCP silencer, Abd-B gene, Pleiohomeotic, GAF, PcG, trxG, Drosophila melanogaster




This article has been cited by other articles:


Home page
 DevelopmentHome page
S. Petruk, S. T. Smith, Y. Sedkov, and A. Mazo
Association of trxG and PcG proteins with the bxd maintenance element depends on transcriptional activity
Development, July 15, 2008; 135(14): 2383 - 2390.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Gohl, M. Muller, V. Pirrotta, M. Affolter, and P. Schedl
Enhancer Blocking and Transvection at the Drosophila apterous Locus
Genetics, January 1, 2008; 178(1): 127 - 143.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
O. Kyrchanova, S. Toshchakov, A. Parshikov, and P. Georgiev
Study of the Functional Interaction between Mcp Insulators from the Drosophila bithorax Complex: Effects of Insulator Pairing on Enhancer-Promoter Communication
Mol. Cell. Biol., April 15, 2007; 27(8): 3035 - 3043.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. H. Wilkinson, K. Park, and M. L. Atchison
Polycomb recruitment to DNA in vivo by the YY1 REPO domain
PNAS, December 19, 2006; 103(51): 19296 - 19301.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. G. Kahn, Y. B. Schwartz, G. I. Dellino, and V. Pirrotta
Polycomb Complexes and the Propagation of the Methylation Mark at the Drosophila Ubx Gene
J. Biol. Chem., September 29, 2006; 281(39): 29064 - 29075.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Mihaly, S. Barges, L. Sipos, R. Maeda, F. Cleard, I. Hogga, W. Bender, H. Gyurkovics, and F. Karch
Dissecting the regulatory landscape of the Abd-B gene of the bithorax complex
Development, August 1, 2006; 133(15): 2983 - 2993.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Klymenko, B. Papp, W. Fischle, T. Kocher, M. Schelder, C. Fritsch, B. Wild, M. Wilm, and J. Muller
A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities
Genes & Dev., May 1, 2006; 20(9): 1110 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. K. Maeda and F. Karch
The ABC of the BX-C: the bithorax complex explained.
Development, April 1, 2006; 133(8): 1413 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A.-M. Martinez, S. Colomb, J. Dejardin, F. Bantignies, and G. Cavalli
Polycomb group-dependent Cyclin A repression in Drosophila.
Genes & Dev., February 15, 2006; 20(4): 501 - 513.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. L. Brown, D. J. Grau, S. K. DeVido, and J. A. Kassis
An Sp1/KLF binding site is important for the activity of a Polycomb group response element from the Drosophila engrailed gene
Nucleic Acids Res., September 9, 2005; 33(16): 5181 - 5189.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Canudas, S. Perez, L. Fanti, S. Pimpinelli, N. Singh, S. D. Hanes, F. Azorin, and M. L. Espinas
dSAP18 and dHDAC1 contribute to the functional regulation of the Drosophila Fab-7 element
Nucleic Acids Res., August 30, 2005; 33(15): 4857 - 4864.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Gruzdeva, O. Kyrchanova, A. Parshikov, A. Kullyev, and P. Georgiev
The Mcp Element from the bithorax Complex Contains an Insulator That Is Capable of Pairwise Interactions and Can Facilitate Enhancer-Promoter Communication
Mol. Cell. Biol., May 1, 2005; 25(9): 3682 - 3689.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Melnikova, F. Juge, N. Gruzdeva, A. Mazur, G. Cavalli, and P. Georgiev
Interaction between the GAGA factor and Mod(mdg4) proteins promotes insulator bypass in Drosophila
PNAS, October 12, 2004; 101(41): 14806 - 14811.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
A. Kirmizis and P. J. Farnham
Genomic Approaches That Aid in the Identification of Transcription Factor Target Genes
Experimental Biology and Medicine, September 1, 2004; 229(8): 705 - 721.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. K. Sengupta, A. Kuhrs, and J. Muller
General transcriptional silencing by a Polycomb response element in Drosophila
Development, May 1, 2004; 131(9): 1959 - 1965.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
N. M. Mulholland, I. F.G. King, and R. E. Kingston
Regulation of Polycomb group complexes by the sequence-specific DNA binding proteins Zeste and GAGA
Genes & Dev., November 15, 2003; 17(22): 2741 - 2746.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. S. Toker, Y. Teng, H. B. Ferreira, S. W. Emmons, and M. Chalfie
The Caenorhabditis elegans spalt-like gene sem-4 restricts touch cell fate by repressing the selector Hox gene egl-5 and the effector gene mec-3
Development, August 15, 2003; 130(16): 3831 - 3840.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Mahmoudi, L. M. P. Zuijderduijn, A. Mohd-Sarip, and C. P. Verrijzer
GAGA facilitates binding of Pleiohomeotic to a chromatinized Polycomb response element
Nucleic Acids Res., July 15, 2003; 31(14): 4147 - 4156.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Salvaing, A. Lopez, A. Boivin, J. S. Deutsch, and F. Peronnet
The Drosophila Corto protein interacts with Polycomb-group proteins and the GAGA factor
Nucleic Acids Res., June 1, 2003; 31(11): 2873 - 2882.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Poux, B. Horard, C. J. A. Sigrist, and V. Pirrotta
The Drosophila Trithorax protein is a coactivator required to prevent re-establishment of Polycomb silencing
Development, March 7, 2003; 129(10): 2483 - 2493.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. L. Brown, C. Fritsch, J. Mueller, and J. A. Kassis
The Drosophila pho-like gene encodes a YY1-related DNA binding protein that is redundant with pleiohomeotic in homeotic gene silencing
Development, March 2, 2003; 130(2): 285 - 294.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Faucheux, J.-Y. Roignant, S. Netter, J. Charollais, C. Antoniewski, and L. Theodore
batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor
Mol. Cell. Biol., February 15, 2003; 23(4): 1181 - 1195.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Rank, M. Prestel, and R. Paro
Transcription through Intergenic Chromosomal Memory Elements of the Drosophila Bithorax Complex Correlates with an Epigenetic Switch
Mol. Cell. Biol., November 15, 2002; 22(22): 8026 - 8034.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Mohd-Sarip, F. Venturini, G. E. Chalkley, and C. P. Verrijzer
Pleiohomeotic Can Link Polycomb to DNA and Mediate Transcriptional Repression
Mol. Cell. Biol., November 1, 2002; 22(21): 7473 - 7483.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kosoy, S. Pagans, M. L. Espinas, F. Azorin, and J. Bernues
GAGA Factor Down-regulates Its Own Promoter
J. Biol. Chem., October 25, 2002; 277(44): 42280 - 42288.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. Maurange and R. Paro
A cellular memory module conveys epigenetic inheritance of hedgehog expression during Drosophila wing imaginal disc development
Genes & Dev., October 15, 2002; 16(20): 2672 - 2683.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Schwendemann and M. Lehmann
Pipsqueak and GAGA factor act in concert as partners at homeotic and many other loci
PNAS, October 1, 2002; 99(20): 12883 - 12888.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. C. Sheldon, A. B. Conn, E. S. Dennis, and W. J. Peacock
Different Regulatory Regions Are Required for the Vernalization-Induced Repression of FLOWERING LOCUS C and for the Epigenetic Maintenance of Repression
PLANT CELL, October 1, 2002; 14(10): 2527 - 2537.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. A. Leibovitch, Q. Lu, L. R. Benjamin, Y. Liu, D. S. Gilmour, and S. C. R. Elgin
GAGA Factor and the TFIID Complex Collaborate in Generating an Open Chromatin Structure at the Drosophila melanogaster hsp26 Promoter
Mol. Cell. Biol., September 1, 2002; 22(17): 6148 - 6157.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Sangwan and M. R. O'Brian
Identification of a Soybean Protein That Interacts with GAGA Element Dinucleotide Repeat DNA
Plant Physiology, August 1, 2002; 129(4): 1788 - 1794.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. Americo, M. Whiteley, J. L. Brown, M. Fujioka, J. B. Jaynes, and J. A. Kassis
A Complex Array of DNA-Binding Proteins Required for Pairing-Sensitive Silencing by a Polycomb Group Response Element From the Drosophila engrailed Gene
Genetics, April 1, 2002; 160(4): 1561 - 1571.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
I. Bajusz, L. Sipos, Z. Gyorgypal, E. A. Carrington, R. S. Jones, J. Gausz, and H. Gyurkovics
The Trithorax-mimic Allele of Enhancer of zeste Renders Active Domains of Target Genes Accessible to Polycomb-Group-Dependent Silencing in Drosophila melanogaster
Genetics, November 1, 2001; 159(3): 1135 - 1150.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001