|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720, USA
*Author for correspondence (e-mail: harland{at}socrates.berkeley.edu)
Accepted April 16, 2001
During amphibian development, non-canonical Wnt signals regulate the polarity of intercalating dorsal mesoderm cells during convergent extension. Cells of the overlying posterior neural ectoderm engage in similar morphogenetic cell movements. Important differences have been discerned in the cell behaviors associated with neural and mesodermal cell intercalation, raising the possibility that different mechanisms may control intercalations in these two tissues. In this report, targeted expression of mutants of Xenopus Dishevelled (Xdsh) to neural or mesodermal tissues elicited different defects that were consistent with inhibition of either neural or mesodermal convergent extension. Expression of mutant Xdsh also inhibited elongation of neural tissues in vitro in Keller sandwich explants and in vivo in neural plate grafts. Targeted expression of other Wnt signaling antagonists also inhibited neural convergent extension in whole embryos. In situ hybridization indicated that these defects were not due to changes in cell fate. Examination of embryonic phenotypes after inhibition of convergent extension in different tissues reveals a primary role for mesodermal convergent extension in axial elongation, and a role for neural convergent extension as an equalizing force to produce a straight axis. This study demonstrates that non-canonical Wnt signaling is a common mechanism controlling convergent extension in two very different tissues in the Xenopus embryo and may reflect a general conservation of control mechanisms in vertebrate convergent extension.
Key words: Dishevelled, Wnt, Convergent extension, Neural tube defects, Xenopus
This article has been cited by other articles:
|
|
 |
|
  W. Liu, A. Sato, D. Khadka, R. Bharti, H. Diaz, L. W. Runnels, and R. Habas Mechanism of activation of the Formin protein Daam1 PNAS, January 8, 2008; 105(1): 210 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Tahinci, C. A. Thorne, J. L. Franklin, A. Salic, K. M. Christian, L. A. Lee, R. J. Coffey, and E. Lee Lrp6 is required for convergent extension during Xenopus gastrulation Development, November 15, 2007; 134(22): 4095 - 4106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Haremaki, S. T. Fraser, Y.-M. Kuo, M. H. Baron, and D. C. Weinstein Vertebrate Ctr1 coordinates morphogenesis and progenitor cell fate and regulates embryonic stem cell differentiation PNAS, July 17, 2007; 104(29): 12029 - 12034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ybot-Gonzalez, D. Savery, D. Gerrelli, M. Signore, C. E. Mitchell, C. H. Faux, N. D. E. Greene, and A. J. Copp Convergent extension, planar-cell-polarity signalling and initiation of mouse neural tube closure Development, February 15, 2007; 134(4): 789 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Borchers, Y. Fonar, D. Frank, and J. C. Baker XNF-ATc3 affects neural convergent extension Development, May 1, 2006; 133(9): 1745 - 1755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, N. S. Hamblet, S. Mark, M. E. Dickinson, B. C. Brinkman, N. Segil, S. E. Fraser, P. Chen, J. B. Wallingford, and A. Wynshaw-Boris Dishevelled genes mediate a conserved mammalian PCP pathway to regulate convergent extension during neurulation Development, May 1, 2006; 133(9): 1767 - 1778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Whitaker Calcium at Fertilization and in Early Development Physiol Rev, January 1, 2006; 86(1): 25 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Wallingford and R. Habas The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity Development, October 15, 2005; 132(20): 4421 - 4436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Endo, V. Wolf, K. Muraiso, K. Kamijo, L. Soon, A. Uren, M. Barshishat-Kupper, and J. S. Rubin Wnt-3a-dependent Cell Motility Involves RhoA Activation and Is Specifically Regulated by Dishevelled-2 J. Biol. Chem., January 7, 2005; 280(1): 777 - 786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Burstyn-Cohen, J. Stanleigh, D. Sela-Donenfeld, and C. Kalcheim Canonical Wnt activity regulates trunk neural crest delamination linking BMP/noggin signaling with G1/S transition Development, November 1, 2004; 131(21): 5327 - 5339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. F. Leise III and P. R. Mueller Inhibition of the cell cycle is required for convergent extension of the paraxial mesoderm during Xenopus neurulation Development, April 15, 2004; 131(8): 1703 - 1715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Aamar and D. Frank Xenopus Meis3 protein forms a hindbrain-inducing center by activating FGF/MAP kinase and PCP pathways Development, January 1, 2004; 131(1): 153 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, J. Wu, C. Tan, and P. S. Klein PP2A:B56{epsilon} is required for Wnt/{beta}-catenin signaling during embryonic development Development, December 1, 2003; 130(23): 5569 - 5578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yaniv, A. Fainsod, C. Kalcheim, and J. K. Yisraeli The RNA-binding protein Vg1 RBP is required for cell migration during early neural development Development, December 1, 2003; 130(23): 5649 - 5661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Strutt Frizzled signalling and cell polarisation in Drosophila and vertebrates Development, October 1, 2003; 130(19): 4501 - 4513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ohkawara, T. S. Yamamoto, M. Tada, and N. Ueno Role of glypican 4 in the regulation of convergent extension movements during gastrulation in Xenopus laevis Development, May 15, 2003; 130(10): 2129 - 2138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yokota, M. Kofron, M. Zuck, D. W. Houston, H. Isaacs, M. Asashima, C. C. Wylie, and J. Heasman A novel role for a nodal-related protein; Xnr3 regulates convergent extension movements via the FGF receptor Development, May 15, 2003; 130(10): 2199 - 2212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Kwan and M. W. Kirschner Xbra functions as a switch between cell migration and convergent extension in the Xenopus gastrula Development, May 1, 2003; 130(9): 1961 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Wallingford and R. M. Harland Neural tube closure requires Dishevelled-dependent convergent extension of the midline Development, March 14, 2003; 129(24): 5815 - 5825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Hamblet, N. Lijam, P. Ruiz-Lozano, J. Wang, Y. Yang, Z. Luo, L. Mei, K. R. Chien, D. J. Sussman, and A. Wynshaw-Boris Dishevelled 2 is essential for cardiac outflow tract development, somite segmentation and neural tube closure Development, March 14, 2003; 129(24): 5827 - 5838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hikasa, M. Shibata, I. Hiratani, and M. Taira The Xenopus receptor tyrosine kinase Xror2 modulates morphogenetic movements of the axial mesoderm and neuroectoderm via Wnt signaling Development, March 13, 2003; 129(22): 5227 - 5239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Glickman, C. B. Kimmel, M. A. Jones, and R. J. Adams Shaping the zebrafish notochord Development, March 1, 2003; 130(5): 873 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lee and P. N. Adler The Function of the frizzled Pathway in the Drosophila Wing Is Dependent on inturned and fuzzy Genetics, April 1, 2002; 160(4): 1535 - 1547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O'N. Wiggan and P. A. Hamel Pax3 regulates morphogenetic cell behavior in vitro coincident with activation of a PCP/non-canonical Wnt-signaling cascade J. Cell Sci., January 2, 2002; 115(3): 531 - 541. [Abstract] [Full Text] [PDF]
|
|
