|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
1 Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
2 Department of Immunology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
3 Department of Immunology, National Jewish Medical and Research Center, Denver, CO 80206, USA
*Author for correspondence (e-mail: chamberlin.27{at}osu.edu)
Accepted May 14, 2001
The Pax gene egl-38 plays an important role in the development of several organs in C. elegans. To understand how a Pax transcription factor influences distinct developmental choices in different cells and tissue types, we have characterized a second gene, lin-48. lin-48 functions with egl-38 in the development of one structure, the hindgut, but not in other tissues such as the egg-laying system. We show that lin-48 encodes a C2H2 zinc-finger protein that is similar to the product of the Drosophila gene ovo and is expressed in the hindgut cells that develop abnormally in lin-48 mutants. We present evidence that lin-48 is a target for EGL-38 in hindgut cells. We show that lin-48 requires egl-38 for its expression in the hindgut. Using deletion analysis, we have identified two elements in the lin-48 promoter that are necessary for lin-48 expression. We demonstrate that EGL-38 binds with high affinity to one of these elements. In addition, we have observed genetic interactions between mutations in the lin-48 promoter and specific alleles of egl-38. These experiments demonstrate a functional link between Pax and Ovo transcription factors, and provide a model for how Pax transcription factors can regulate different target genes in different cells.
Key words: Organogenesis, Pax2, Paired domain, C. elegans
This article has been cited by other articles:
|
|
 |
|
  J. Milloz, F. Duveau, I. Nuez, and M.-A. Felix Intraspecific evolution of the intercellular signaling network underlying a robust developmental system Genes & Dev., November 1, 2008; 22(21): 3064 - 3075. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Rocheleau, K. Cullison, K. Huang, Y. Bernstein, A. C. Spilker, and M. V. Sundaram The Caenorhabditis elegans ekl (Enhancer of ksr-1 Lethality) Genes Include Putative Components of a Germline Small RNA Pathway Genetics, March 1, 2008; 178(3): 1431 - 1443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sun, B. L. Nelms, S. F. Sleiman, H. M. Chamberlin, and W. Hanna-Rose Modulation of Caenorhabditis elegans Transcription Factor Activity by HIM-8 and the Related Zinc-Finger ZIM Proteins Genetics, October 1, 2007; 177(2): 1221 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nair, V. Bilanchone, K. Ortt, S. Sinha, and X. Dai Ovol1 represses its own transcription by competing with transcription activator c-Myb and by recruiting histone deacetylase activity Nucleic Acids Res., March 12, 2007; 35(5): 1687 - 1697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nair, A. Teng, V. Bilanchone, A. Agrawal, B. Li, and X. Dai Ovol1 regulates the growth arrest of embryonic epidermal progenitor cells and represses c-myc transcription J. Cell Biol., April 24, 2006; 173(2): 253 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Berset, E. F. Hoier, and A. Hajnal The C. elegans homolog of the mammalian tumor suppressor Dep-1/Scc1 inhibits EGFR signaling to regulate binary cell fate decisions Genes & Dev., June 1, 2005; 19(11): 1328 - 1340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Li, M. Nair, D. R. Mackay, V. Bilanchone, M. Hu, M. Fallahi, H. Song, Q. Dai, P. E. Cohen, and X. Dai Ovol1 regulates meiotic pachytene progression during spermatogenesis by repressing Id2 expression Development, March 15, 2005; 132(6): 1463 - 1473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang and H. M. Chamberlin Multiple regulatory changes contribute to the evolution of the Caenorhabditis lin-48 ovo gene Genes & Dev., September 15, 2002; 16(18): 2345 - 2349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lints and S. W. Emmons Regulation of sex-specific differentiation and mating behavior in C. elegans by a new member of the DM domain transcription factor family Genes & Dev., September 15, 2002; 16(18): 2390 - 2402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Li, D. R. Mackay, Q. Dai, T. W. H. Li, M. Nair, M. Fallahi, C. P. Schonbaum, J. Fantes, A. P. Mahowald, M. L. Waterman, et al. The LEF1/beta -catenin complex activates movo1, a mouse homolog of Drosophila ovo required for epidermal appendage differentiation PNAS, April 30, 2002; 99(9): 6064 - 6069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Fitzsimmons, R. Lutz, W. Wheat, H. M. Chamberlin, and J. Hagman Highly conserved amino acids in Pax and Ets proteins are required for DNA binding and ternary complex assembly Nucleic Acids Res., October 15, 2001; 29(20): 4154 - 4165. [Abstract] [Full Text] [PDF]
|
|
