spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wei, L.
Right arrow Articles by Imanaka-Yoshida, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wei, L.
Right arrow Articles by Imanaka-Yoshida, K.
Development 128, 2953-2962 (2001)
© 2001 The Company of Biologists Limited

Rho kinases play an obligatory role in vertebrate embryonic organogenesis

Lei Wei1,2,4,*, Wilmer Roberts1,*, Lu Wang2,4, Miho Yamada1, Shuxing Zhang1, Zhiyong Zhao5, Scott A. Rivkees5, Robert J. Schwartz1,2,3,4,{ddagger} and Kyoko Imanaka-Yoshida6

1 Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
2 Cardiovascular Sciences Section, Baylor College of Medicine, Houston, TX 77030, USA
3 Center for Cardiovascular Development, Baylor College of Medicine, Houston, TX 77030, USA
4 Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
5 Yale Child Health Research Center, Yale University School of Medicine, New Haven, CN, USA
6 Department of Pathology, Mie University, School of Medicine, Tsu, Mie, Japan
* These authors contributed equally to this work

{ddagger}Author for correspondence (e-mail: schwartz{at}bcm.tmc.edu)

Accepted May 12, 2001

Rho-associated kinases (Rho kinases), which are downstream effectors of RhoA GTPase, regulate diverse cellular functions including actin cytoskeletal organization. We have demonstrated that Rho kinases also direct the early stages of chick and mouse embryonic morphogenesis. We observed that Rho kinase transcripts were enriched in cardiac mesoderm, lateral plate mesoderm and the neural plate. Treatment of neurulating embryos with Y27632, a specific inhibitor of Rho kinases, blocked migration and fusion of the bilateral heart primordia, formation of the brain and neural tube, caudalward movement of Hensen’s node, and establishment of normal left-right asymmetry. Moreover, Y27632 induced precocious expression of cardiac {alpha}-actin, an early marker of cardiomyocyte differentiation, coincident with the upregulated expression of serum response factor and GATA4. In addition, specific antisense oligonucleotides significantly diminished Rho kinase mRNA levels and replicated many of the teratologies induced by Y27632. Thus, our study reveals new biological functions for Rho kinases in regulating major morphogenetic events during early chick and mouse development.

Key words: Rho kinase, Y27632, Cardia bifida, Cardiomyocyte differentiation, Chick




This article has been cited by other articles:


Home page
 DevelopmentHome page
T. Nishimura and M. Takeichi
Shroom3-mediated recruitment of Rho kinases to the apical cell junctions regulates epithelial and neuroepithelial planar remodeling
Development, April 15, 2008; 135(8): 1493 - 1502.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Q. Yue, L. Wagstaff, X. Yang, C. Weijer, and A. Munsterberg
Wnt3a-mediated chemorepulsion controls movement patterns of cardiac progenitors and requires RhoA function
Development, March 15, 2008; 135(6): 1029 - 1037.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
A. Schmandke, A. Schmandke, and S. M. Strittmatter
ROCK and Rho: Biochemistry and Neuronal Functions of Rho-Associated Protein Kinases
Neuroscientist, October 1, 2007; 13(5): 454 - 469.
[Abstract] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Pelosi, F. Marampon, B. M. Zani, S. Prudente, E. Perlas, V. Caputo, L. Cianetti, V. Berno, S. Narumiya, S. W. Kang, et al.
ROCK2 and Its Alternatively Spliced Isoform ROCK2m Positively Control the Maturation of the Myogenic Program
Mol. Cell. Biol., September 1, 2007; 27(17): 6163 - 6176.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Barrientos, D. Frank, K. Kuwahara, S. Bezprozvannaya, G. C. T. Pipes, R. Bassel-Duby, J. A. Richardson, H. A. Katus, E. N. Olson, and N. Frey
Two Novel Members of the ABLIM Protein Family, ABLIM-2 and -3, Associate with STARS and Directly Bind F-actin
J. Biol. Chem., March 16, 2007; 282(11): 8393 - 8403.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. R. Panizzi, J. R. Jessen, I. A. Drummond, and L. Solnica-Krezel
New functions for a vertebrate Rho guanine nucleotide exchange factor in ciliated epithelia
Development, March 1, 2007; 134(5): 921 - 931.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. Ybot-Gonzalez, D. Savery, D. Gerrelli, M. Signore, C. E. Mitchell, C. H. Faux, N. D. E. Greene, and A. J. Copp
Convergent extension, planar-cell-polarity signalling and initiation of mouse neural tube closure
Development, February 15, 2007; 134(4): 789 - 799.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Noma, N. Oyama, and J. K. Liao
Physiological role of ROCKs in the cardiovascular system
Am J Physiol Cell Physiol, March 1, 2006; 290(3): C661 - C668.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
G. Loirand, P. Guerin, and P. Pacaud
Rho Kinases in Cardiovascular Physiology and Pathophysiology
Circ. Res., February 17, 2006; 98(3): 322 - 334.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. D. Hildebrand
Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network
J. Cell Sci., November 15, 2005; 118(22): 5191 - 5203.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Niu, W. Yu, S. X. Zhang, M. Barron, N. S. Belaguli, M. D. Schneider, M. Parmacek, A. Nordheim, and R. J. Schwartz
Conditional Mutagenesis of the Murine Serum Response Factor Gene Blocks Cardiogenesis and the Transcription of Downstream Gene Targets
J. Biol. Chem., September 16, 2005; 280(37): 32531 - 32538.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
H. M. Phillips, J. N. Murdoch, B. Chaudhry, A. J. Copp, and D. J. Henderson
Vangl2 Acts via RhoA Signaling to Regulate Polarized Cell Movements During Development of the Proximal Outflow Tract
Circ. Res., February 18, 2005; 96(3): 292 - 299.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Matsui, A. Raya, Y. Kawakami, C. Callol-Massot, J. Capdevila, C. Rodriguez-Esteban, and J. C. Izpisua Belmonte
Noncanonical Wnt signaling regulates midline convergence of organ primordia during zebrafish development
Genes & Dev., January 1, 2005; 19(1): 164 - 175.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nishiyama, I. Kii, and A. Kudo
Inactivation of Rho/ROCK Signaling Is Crucial for the Nuclear Accumulation of FKHR and Myoblast Fusion
J. Biol. Chem., November 5, 2004; 279(45): 47311 - 47319.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Kobayashi, M. Takahashi, N. Matsushita, J.-i. Miyazaki, M. Koike, H. Yaginuma, N. Osumi, K. Kaibuchi, and K. Kobayashi
Survival of Developing Motor Neurons Mediated by Rho GTPase Signaling Pathway through Rho-Kinase
J. Neurosci., April 7, 2004; 24(14): 3480 - 3488.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Matsuura, T. Nagai, N. Nishigaki, T. Oyama, J. Nishi, H. Wada, M. Sano, H. Toko, H. Akazawa, T. Sato, et al.
Adult Cardiac Sca-1-positive Cells Differentiate into Beating Cardiomyocytes
J. Biol. Chem., March 19, 2004; 279(12): 11384 - 11391.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. E. Ward, J. Evans, and C. S. Thummel
Genetic Modifier Screens in Drosophila Demonstrate a Role for Rho1 Signaling in Ecdysone-Triggered Imaginal Disc Morphogenesis
Genetics, November 1, 2003; 165(3): 1397 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Thumkeo, J. Keel, T. Ishizaki, M. Hirose, K. Nonomura, H. Oshima, M. Oshima, M. M. Taketo, and S. Narumiya
Targeted Disruption of the Mouse Rho-Associated Kinase 2 Gene Results in Intrauterine Growth Retardation and Fetal Death
Mol. Cell. Biol., July 15, 2003; 23(14): 5043 - 5055.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B. G. Bruneau
Transcriptional Regulation of Vertebrate Cardiac Morphogenesis
Circ. Res., March 22, 2002; 90(5): 509 - 519.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Beqaj, S. Jakkaraju, R. R. Mattingly, D. Pan, and L. Schuger
High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis
J. Cell Biol., March 4, 2002; 156(5): 893 - 903.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L. Wei, K. Imanaka-Yoshida, L. Wang, S. Zhan, M. D. Schneider, F. J. DeMayo, and R. J. Schwartz
Inhibition of Rho family GTPases by Rho GDP dissociation inhibitor disrupts cardiac morphogenesis and inhibits cardiomyocyte proliferation
Development, January 4, 2002; 129(7): 1705 - 1714.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Beqaj, S. Jakkaraju, R. R. Mattingly, D. Pan, and L. Schuger
High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis
J. Cell Biol., March 4, 2002; 156(5): 893 - 903.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001