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Development 128, 3197-3207 (2001)
© 2001 The Company of Biologists Limited

Functional specificity of the Hoxa13 homeobox

Yuanxiang Zhao and S. Steven Potter*

Division of Developmental Biology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45224, USA
* Author for correspondence (e-mail: steve.potter{at}chmcc.org )

Accepted 30 May 2001

To better define Abd-B type homeodomain function, to test models that predict functional equivalence of all Hox genes and to initiate a search for the downstream targets of Hoxa13, we have performed a homeobox swap by replacing the homeobox of the Hoxa11 gene with that of the Hoxa13 gene. The Hoxa11 and Hoxa13 genes are contiguous Abd-B type genes located at the 5' end of the HoxA cluster. The modified Hoxa11 allele (A1113hd) showed near wild-type function in the development of the kidneys, axial skeleton and male reproductive tract, consistent with functional equivalence models. In the limbs and female reproductive tract, however, the A1113hd allele appeared to assume dominant Hoxa13 function. The uterus, in particular, showed a striking homeotic transformation towards cervix/vagina, where Hoxa13 is normally expressed. Gene chips were used to create a molecular portrait of this tissue conversion and revealed over 100 diagnostic gene expression changes. This work identifies candidate downstream targets of the Hoxa13 gene and demonstrates that even contiguous Abd-B homeoboxes have functional specificity.

Key words: Hox, Hoxa13, Hoxa11, Homeobox, Functional specificity, Abd-B, Downstream targets, Mouse


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© The Company of Biologists Ltd 2001