spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Navarro, R. E.
Right arrow Articles by Blackwell, T. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Navarro, R. E.
Right arrow Articles by Blackwell, T. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Development 128, 3221-3232 (2001)
© 2001 The Company of Biologists Limited

cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans

Rosa E. Navarro1, Eun Yong Shim1,*, Yuji Kohara3, Andrew Singson2 and T. Keith Blackwell1,{ddagger}

1 Center for Blood Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
2 Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
3 Japan Science and Technology Corporation, Genome Biology Laboratory, National Institute of Genetics, Mishima 411-8540, Japan
* This author made an important independent contribution to this work

{ddagger}Author for correspondence (e-mail: blackwell{at}cbr.med.harvard.edu)

Accepted June 11, 2001

A high frequency of apoptosis is a conserved hallmark of oocyte development. In C. elegans, about half of all developing oocytes are normally killed by a physiological germline-specific apoptosis pathway, apparently so that they donate cytoplasm to the survivors. We have investigated the functions of CGH-1, the C. elegans ortholog of the predicted RNA helicase ste13/ME31B/RCK/p54, which is germline-associated in metazoans and required for sexual reproduction in yeast. We show that CGH-1 is expressed specifically in the germline and early embryo, and is localized to P granules and other possible mRNA-protein particles. cgh-1 is required for oocyte and sperm function. It is also needed to prevent the physiological germline apoptosis mechanism killing essentially all developing oocytes, making lack of cgh-1 function the first stimulus identified that can trigger this mechanism. We conclude that cgh-1 and its orthologs may perform conserved functions during gametogenesis, that in C. elegans certain aspects of oocyte development are monitored by the physiological germline apoptosis pathway, and that similar surveillance mechanisms may contribute to germline apoptosis in other species.

Key words: Germline, Apoptosis, RNA helicase, Oocyte, Caenorhabditis elegans, P granules, Germ plasm


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
S. Nadarajan, J. A. Govindan, M. McGovern, E. J. A. Hubbard, and D. Greenstein
MSP and GLP-1/Notch signaling coordinately regulate actomyosin-dependent cytoplasmic streaming and oocyte growth in C. elegans
Development, July 1, 2009; 136(13): 2223 - 2234.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. L. Noble, B. L. Allen, L. K. Goh, K. Nordick, and T. C. Evans
Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development
J. Cell Biol., August 11, 2008; 182(3): 559 - 572.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
P. R. Boag, A. Atalay, S. Robida, V. Reinke, and T. K. Blackwell
Protection of specific maternal messenger RNAs by the P body protein CGH-1 (Dhh1/RCK) during Caenorhabditis elegans oogenesis
J. Cell Biol., August 11, 2008; 182(3): 543 - 557.
[Abstract] [Full Text] [PDF]

Home page
 DNA ResHome page
T. Eki, T. Ishihara, I. Katsura, and F. Hanaoka
A Genome-wide Survey and Systematic RNAi-based Characterization of Helicase-like Genes in Caenorhabditis elegans
DNA Res, October 6, 2007; (2007) dsm016v1.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. M. Orsborn, W. Li, T. J. McEwen, T. Mizuno, E. Kuzmin, K. Matsumoto, and K. L. Bennett
GLH-1, the C. elegans P granule protein, is controlled by the JNK KGB-1 and by the COP9 subunit CSN-5
Development, September 15, 2007; 134(18): 3383 - 3392.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. R. DeBella, A. Hayashi, and L. S. Rose
LET-711, the Caenorhabditis elegans NOT1 Ortholog, Is Required for Spindle Positioning and Regulation of Microtubule Length in Embryos
Mol. Biol. Cell, November 1, 2006; 17(11): 4911 - 4924.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
E. A. Kritikou, S. Milstein, P.-O. Vidalain, G. Lettre, E. Bogan, K. Doukoumetzidis, P. Gray, T. G. Chappell, M. Vidal, and M. O. Hengartner
C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway required for germ cell survival.
Genes & Dev., August 15, 2006; 20(16): 2279 - 2292.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Weston and J. Sommerville
Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradation
Nucleic Acids Res., June 14, 2006; 34(10): 3082 - 3094.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Lall, F. Piano, and R. E. Davis
Caenorhabditis elegans Decapping Proteins: Localization and Functional Analysis of Dcp1, Dcp2, and DcpS during Embryogenesis
Mol. Biol. Cell, December 1, 2005; 16(12): 5880 - 5890.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. R. Boag, A. Nakamura, and T. K. Blackwell
A conserved RNA-protein complex component involved in physiological germline apoptosis regulation in C. elegans
Development, November 15, 2005; 132(22): 4975 - 4986.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Audhya, F. Hyndman, I. X. McLeod, A. S. Maddox, J. R. Yates III, A. Desai, and K. Oegema
A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans
J. Cell Biol., October 24, 2005; 171(2): 267 - 279.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
Z. CHENG, J. COLLER, R. PARKER, and H. SONG
Crystal structure and functional analysis of DEAD-box protein Dhh1p
RNA, August 1, 2005; 11(8): 1258 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Minshall and N. Standart
The active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer
Nucleic Acids Res., February 24, 2004; 32(4): 1325 - 1334.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
U. Sheth and R. Parker
Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies
Science, May 2, 2003; 300(5620): 805 - 808.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. A. Smillie and J. Sommerville
RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles
J. Cell Sci., January 15, 2002; 115(2): 395 - 407.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. L. Western, Y. Cheng, J. Liu, and X. Chen
HUA ENHANCER2, a putative DExH-box RNA helicase, maintains homeotic B and C gene expression in Arabidopsis
Development, January 4, 2002; 129(7): 1569 - 1581.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001