|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
1 Department of Genetics, Umeå University, S-90187 Umeå, Sweden
2 Max Planck Institute for Developmental Biology, Spemannstrasse 35/III, 72076, Tübingen, Germany
3 Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2785, USA
*Author for correspondence (e-mail: juerg.mueller{at}tuebingen.mpg.de)
Accepted June 22, 2001
In both Drosophila and vertebrates, spatially restricted expression of HOX genes is controlled by the Polycomb group (PcG) repressors. Here we characterize a novel Drosophila PcG gene, Suppressor of zeste 12 (Su(z)12). Su(z)12 mutants exhibit very strong homeotic transformations and Su(z)12 function is required throughout development to maintain the repressed state of HOX genes. Unlike most other PcG mutations, Su(z)12 mutations are strong suppressors of position-effect variegation (PEV), suggesting that Su(z)12 also functions in heterochromatin-mediated repression. Furthermore, Su(z)12 function is required for germ cell development. The Su(z)12 protein is highly conserved in vertebrates and is related to the Arabidopsis proteins EMF2, FIS2 and VRN2. Notably, EMF2 is a repressor of floral homeotic genes. These results suggest that at least some of the regulatory machinery that controls homeotic gene expression is conserved between animals and plants.
Key words: Su(z)12, Polycomb group, Drosophila melanogaster
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Miro, X. Zhou, S. Boretius, T. Michaelis, C. Kubisch, G. Alvarez-Bolado, and P. Gruss Haploinsufficiency of the murine polycomb gene Suz12 results in diverse malformations of the brain and neural tube Dis. Model. Mech., July 1, 2009; 2(7-8): 412 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-J. Chen, Z.-Y. Diao, C. Specht, and Z. R. Sung Molecular Evolution of VEF-Domain-Containing PcG Genes in Plants Mol Plant, July 1, 2009; 2(4): 738 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Joshi, E. A. Carrington, L. Wang, C. S. Ketel, E. L. Miller, R. S. Jones, and J. A. Simon Dominant Alleles Identify SET Domain Residues Required for Histone Methyltransferase of Polycomb Repressive Complex 2 J. Biol. Chem., October 10, 2008; 283(41): 27757 - 27766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li, X. Ma, J. Wang, J. Koontz, M. Nucci, and J. Sklar Effects of rearrangement and allelic exclusion of JJAZ1/SUZ12 on cell proliferation and survival PNAS, December 11, 2007; 104(50): 20001 - 20006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. de Ayala Alonso, L. Gutierrez, C. Fritsch, B. Papp, D. Beuchle, and J. Muller A Genetic Screen Identifies Novel Polycomb Group Genes in Drosophila Genetics, August 1, 2007; 176(4): 2099 - 2108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, S. D. Taverna, T. L. Muratore, J. Shabanowitz, D. F. Hunt, and C. D. Allis RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena Genes & Dev., June 15, 2007; 21(12): 1530 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pasini, A. P. Bracken, J. B. Hansen, M. Capillo, and K. Helin The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation Mol. Cell. Biol., May 15, 2007; 27(10): 3769 - 3779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Wood, M. Robertson, G. Tanner, W. J. Peacock, E. S. Dennis, and C. A. Helliwell The Arabidopsis thaliana vernalization response requires a polycomb-like protein complex that also includes VERNALIZATION INSENSITIVE 3 PNAS, September 26, 2006; 103(39): 14631 - 14636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Qi, H. Jin, T. Lilja, and M. Mannervik Drosophila Reptin and Other TIP60 Complex Components Promote Generation of Silent Chromatin Genetics, September 1, 2006; 174(1): 241 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wang, M. D. Tyson, S. S. Jackson, and R. Yadegari Partially redundant functions of two SET-domain polycomb-group proteins in controlling initiation of seed development in Arabidopsis PNAS, August 29, 2006; 103(35): 13244 - 13249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Papp and J. Muller Histone trimethylation and the maintenance of transcriptional ONand OFF states by trxG and PcG proteins Genes & Dev., August 1, 2006; 20(15): 2041 - 2054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Klymenko, B. Papp, W. Fischle, T. Kocher, M. Schelder, C. Fritsch, B. Wild, M. Wilm, and J. Muller A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities Genes & Dev., May 1, 2006; 20(9): 1110 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhu, J. Sen, L. Stevens, J. S. Goltz, and D. Stein Drosophila Pipe protein activity in the ovary and the embryonic salivary gland does not require heparan sulfate glycosaminoglycans Development, September 1, 2005; 132(17): 3813 - 3822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Ketel, E. F. Andersen, M. L. Vargas, J. Suh, S. Strome, and J. A. Simon Subunit Contributions to Histone Methyltransferase Activities of Fly and Worm Polycomb Group Complexes Mol. Cell. Biol., August 15, 2005; 25(16): 6857 - 6868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Zirin and R. S. Mann Differing strategies for the establishment and maintenance of teashirt and homothorax repression in the Drosophila wing Development, November 15, 2004; 131(22): 5683 - 5693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chanvivattana, A. Bishopp, D. Schubert, C. Stock, Y.-H. Moon, Z. R. Sung, and J. Goodrich Interaction of Polycomb-group proteins controlling flowering in Arabidopsis Development, November 1, 2004; 131(21): 5263 - 5276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Ali and W. Bender Cross-Regulation among the Polycomb Group Genes in Drosophila melanogaster Mol. Cell. Biol., September 1, 2004; 24(17): 7737 - 7747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kirmizis and P. J. Farnham Genomic Approaches That Aid in the Identification of Transcription Factor Target Genes Experimental Biology and Medicine, September 1, 2004; 229(8): 705 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Peterson, D. R. Mallin, N. J. Francis, C. S. Ketel, J. Stamm, R. K. Voeller, R. E. Kingston, and J. A. Simon Requirement for Sex Comb on Midleg Protein Interactions in Drosophila Polycomb Group Repression Genetics, July 1, 2004; 167(3): 1225 - 1239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. Boss, R. M. Bastow, J. S. Mylne, and C. Dean Multiple Pathways in the Decision to Flower: Enabling, Promoting, and Resetting PLANT CELL, June 1, 2004; 16(suppl_1): S18 - S31. [Full Text] [PDF]
|
|
|
|
|
|
M. Gehring, Y. Choi, and R. L. Fischer Imprinting and Seed Development PLANT CELL, June 1, 2004; 16(suppl_1): S203 - S213. [Full Text] [PDF]
|
|
|
|
|
|
A. K. Sengupta, A. Kuhrs, and J. Muller General transcriptional silencing by a Polycomb response element in Drosophila Development, May 1, 2004; 131(9): 1959 - 1965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kozaki, S. Hake, and J. Colasanti The maize ID1 flowering time regulator is a zinc finger protein with novel DNA binding properties Nucleic Acids Res., March 12, 2004; 32(5): 1710 - 1720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamamoto, M. Sonoda, J. Inokuchi, S. Shirasawa, and T. Sasazuki Polycomb Group Suppressor of Zeste 12 Links Heterochromatin Protein 1{alpha} and Enhancer of Zeste 2 J. Biol. Chem., January 2, 2004; 279(1): 401 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. ZHANG, R. CAO, L. WANG, and R.S. JONES Mechanism of Polycomb Group Gene Silencing Cold Spring Harb Symp Quant Biol, January 1, 2004; 69(0): 309 - 318. [Abstract] [PDF]
|
|
|
|
|
|
F. Janody, J. D. Lee, N. Jahren, D. J. Hazelett, A. Benlali, G. I. Miura, I. Draskovic, and J. E. Treisman A Mosaic Genetic Screen Reveals Distinct Roles for trithorax and Polycomb Group Genes in Drosophila Eye Development Genetics, January 1, 2004; 166(1): 187 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Kelley and M. I. Kuroda The Drosophila roX1 RNA Gene Can Overcome Silent Chromatin by Recruiting the Male-Specific Lethal Dosage Compensation Complex Genetics, June 1, 2003; 164(2): 565 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, Y. Terada, I. Nakai, H. Nakanishi, E. Yoshimura, S. Mori, and N. K. Nishizawa Role of Nicotianamine in the Intracellular Delivery of Metals and Plant Reproductive Development PLANT CELL, June 1, 2003; 15(6): 1263 - 1280. [Abstract] [Full Text]
|
|
|
|
|
|
F. Tie, J. Prasad-Sinha, A. Birve, A. Rasmuson-Lestander, and P. J. Harte A 1-Megadalton ESC/E(Z) Complex from Drosophila That Contains Polycomblike and RPD3 Mol. Cell. Biol., May 1, 2003; 23(9): 3352 - 3362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Moon, L. Chen, R. L. Pan, H.-S. Chang, T. Zhu, D. M. Maffeo, and Z. R. Sung EMF Genes Maintain Vegetative Development by Repressing the Flower Program in Arabidopsis PLANT CELL, March 1, 2003; 15(3): 681 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kirmizis, S. M. Bartley, and P. J. Farnham Identification of the Polycomb Group Protein SU(Z)12 as a Potential Molecular Target for Human Cancer Therapy Mol. Cancer Ther., January 1, 2003; 2(1): 113 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kuzmichev, K. Nishioka, H. Erdjument-Bromage, P. Tempst, and D. Reinberg Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein Genes & Dev., November 15, 2002; 16(22): 2893 - 2905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Cao, L. Wang, H. Wang, L. Xia, H. Erdjument-Bromage, P. Tempst, R. S. Jones, and Y. Zhang Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing Science, November 1, 2002; 298(5595): 1039 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Reyes, L. Hennig, and W. Gruissem Chromatin-Remodeling and Memory Factors. New Regulators of Plant Development Plant Physiology, November 1, 2002; 130(3): 1090 - 1101. [Full Text] [PDF]
|
|
|
|
|
|
C. C. Sheldon, A. B. Conn, E. S. Dennis, and W. J. Peacock Different Regulatory Regions Are Required for the Vernalization-Induced Repression of FLOWERING LOCUS C and for the Epigenetic Maintenance of Repression PLANT CELL, October 1, 2002; 14(10): 2527 - 2537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mouradov, F. Cremer, and G. Coupland Control of Flowering Time: Interacting Pathways as a Basis for Diversity PLANT CELL, May 1, 2002; 14(90001): S111 - 130. [Full Text] [PDF]
|
|
|
|
|
|
N. Yoshida, Y. Yanai, L. Chen, Y. Kato, J. Hiratsuka, T. Miwa, Z. R. Sung, and S. Takahashi EMBRYONIC FLOWER2, a Novel Polycomb Group Protein Homolog, Mediates Shoot Development and Flowering in Arabidopsis PLANT CELL, November 1, 2001; 13(11): 2471 - 2481. [Abstract] [Full Text] [PDF]
|
|
