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Development 128, 3649-3654 (2001)
© 2001 The Company of Biologists Limited

Requirements for the adenylyl cyclases in the development of Dictyostelium

Christophe Anjard, Fredrik Söderbom* and William F. Loomis{ddagger}

Center for Molecular Genetics, Division of Biology, University of California San Diego, La Jolla, CA 92093, USA
* Present address: Dept. of Microbiology, Swedish University of Agricultural Sciences Box 7025, 750 07 Uppsala, Sweden

{ddagger}Author for correspondence (e-mail: wloomis{at}ucsd.edu)

Accepted June 19, 2001

It has been suggested that all intracellular signaling by cAMP during development of Dictyostelium is mediated by the cAMP-dependent protein kinase, PKA, since cells carrying null mutations in the acaA gene that encodes adenylyl cyclase can develop so as to form fruiting bodies under some conditions if PKA is made constitutive by overexpressing the catalytic subunit. However, a second adenylyl cyclase encoded by acrA has recently been found that functions in a cell autonomous fashion during late development. We have found that expression of a modified acaA gene rescues acrA- mutant cells indicating that the only role played by ACR is to produce cAMP. To determine whether cells lacking both adenylyl cyclase genes can develop when PKA is constitutive we disrupted acrA in a acaA- PKA-Cover strain. When developed at high cell densities, acrA- acaA- PKA-Cover cells form mounds, express cell type-specific genes at reduced levels and secrete cellulose coats but do not form fruiting bodies or significant numbers of viable spores. Thus, it appears that synthesis of cAMP is required for spore differentiation in Dictyostelium even if PKA activity is high.

Key words: Adenylyl cyclase ACA, Adenylyl cyclase ACR, cAMP-dependent protein kinase PKA, Sporulation, Dictyostelium


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[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001