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1 Institut für Genetik der Freien Universität Berlin, Arnimallee 7, D-14195 Berlin, Germany
2 Howard Hughes Medical Institute, Department of Human Genetics, University of Utah, 15N 2030E, Room 5200, Salt Lake City, UT 84112-5331, USA
*Author for correspondence (e-mail: mlehmann{at}howard.genetics.utah.edu)
Accepted July 6, 2001
Drosophila development is coordinated by pulses of the steroid hormone 20-hydroxyecdysone (20E). During metamorphosis, the 20E-inducible Broad-Complex (BR-C) gene plays a key role in the genetic hierarchies that transduce the hormone signal, being required for the destruction of larval tissues and numerous aspects of adult development. Most of the known BR-C target genes, including the salivary gland secretion protein (Sgs) genes, are terminal differentiation genes that are thought to be directly regulated by BR-C-encoded transcription factors. Here, we show that repression of Sgs expression is indirectly controlled by the BR-C through transcriptional down-regulation of fork head, a tissue-specific gene that plays a central role in salivary gland development and is required for Sgs expression. Our results demonstrate that integration of a tissue-specific regulatory gene into a 20E-controlled genetic hierarchy provides a mechanism for hormonal repression. Furthermore, they suggest that the BR-C is placed at a different position within the 20E-controlled hierarchies than previously assumed, and that at least part of its pleiotropic functions are mediated by tissue-specific regulators.
Key words: Broad-Complex, Fork head, Negative hormone response, Sgs genes, Steroid hormone, Transcription, Drosophila
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