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Medaka eyeless is the key factor linking retinal determination and eye growth

Felix Loosli¹, Sylke Winkler¹, Carola Burgtorf¹, Elisa Wurmbach^2,*, Wilhelm Ansorge², Thorsten Henrich^1,, Clemens Grabher¹, Detlev Arendt¹, Matthias Carl¹, Annette Krone¹, Erika Grzebisz¹ and Joachim Wittbrodt^1,

¹ European Molecular Biology Laboratory, Developmental Biology Programme, Meyerhofstr. 1, 69117 Heidelberg, Germany
² European Molecular Biology Laboratory, Biochemical Instrumentation Programme, Meyerhofstr. 1, 69117 Heidelberg, Germany
^* Present address: Mount Sinai School of Medicine, New York, NY 10029, USA
Present address: Kondoh differentiation signalling project, Kyoto, Japan

Author for correspondence (e-mail: Jochen.Wittbrodt{at}EMBL-Heidelberg.de)

Accepted July 16, 2001

The complete absence of eyes in the medaka fish mutation eyeless is the result of defective optic vesicle evagination. We show that the eyeless mutation is caused by an intronic insertion in the Rx3 homeobox gene resulting in a transcriptional repression of the locus that is rescued by injection of plasmid DNA containing the wild-type locus. Functional analysis reveals that Six3- and Pax6- dependent retina determination does not require Rx3. However, gain- and loss-of-function phenotypes show that Rx3 is indispensable to initiate optic vesicle evagination and to control vesicle proliferation, by that regulating organ size. Thus, Rx3 acts at a key position coupling the determination with subsequent morphogenesis and differentiation of the developing eye.

Key words: Retina determination, Morphogenesis, Proliferation, Size control, Zebrafish

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