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The protein kinase Pelle mediates feedback regulation in the Drosophila Toll signaling pathway

¹ Section of Cell and Developmental Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0634, USA
² Department of Biochemistry and Molecular Biology, University of Texas, M.D. Anderson Cancer Center, Houston, Texas 77030-4095, USA

*Author for correspondence (e-mail: sawasserman{at}ucsd.edu)

Accepted September 13, 2001

Dorsoventral polarity in the Drosophila embryo is established through a signal transduction cascade triggered in ventral and ventrolateral regions. Activation of a transmembrane receptor, Toll, leads to localized recruitment of the adaptor protein Tube and protein kinase Pelle. Signaling through these components directs degradation of the IB-like inhibitor Cactus and nuclear translocation of the Rel protein Dorsal. Here we show through confocal immunofluorescence microscopy that Pelle functions to downregulate the signal-dependent relocalization of Tube. Inactivation of the Pelle kinase domain, or elimination of the Tube-Pelle interaction, dramatically increases Tube recruitment to the ventral plasma membrane in regions of active signaling. We also characterize a large collection of pelle alleles, identifying the molecular lesions in these alleles and their effects on Pelle autophosphorylation, Tube phosphorylation and Tube relocalization. Our results point to a mechanism operating to modulate the domain or duration of signaling downstream from Tube and Pelle.

Key words: Patterning, Dorsal, Pelle, Dorsoventral polarity, Drosophila melanogaster

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