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Development, Vol 128, Issue 3 365-375, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
EW Howard, LA Newman, DW Oleksyn, RC Angerer and LM Angerer
Department of Biology, University of Rochester, Rochester, NY 14627, USA.
Localization of nuclear beta-catenin initiates specification of vegetal fates in sea urchin embryos. We have identified SpKrl, a gene that is activated upon nuclear entry of beta-catenin. SpKrl is upregulated when nuclear beta-catenin activity is increased with LiCl and downregulated in embryos injected with molecules that inhibit beta-catenin nuclear function. LiCl-mediated SpKrl activation is independent of protein synthesis, indicating that SpKrl is a direct target of beat-catenin and TCF. Embryos in which SpKrl translation is inhibited with morpholino antisense oligonucleotides lack endoderm. Conversely, SpKrl mRNA injection rescues some vegetal structures in beta-catenin-deficient embryos. SpKrl negatively regulates expression of the animalizing transcription factor, SpSoxB1. We propose that SpKrl functions in patterning the vegetal domain by suppressing animal regulatory activities.
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 D. Y.R. Stainier A glimpse into the molecular entrails of endoderm formation Genes & Dev., April 15, 2002; 16(8): 893 - 907. [Full Text] [PDF]
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L. M. Angerer, D. W. Oleksyn, A. M. Levine, X. Li, W. H. Klein, and R. C. Angerer Sea urchin goosecoid function links fate specification along the animal-vegetal and oral-aboral embryonic axes Development, November 15, 2001; 128(22): 4393 - 4404. [Abstract] [Full Text] [PDF]
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