|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 128, Issue 6 929-941, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
GI Gallicano, C Bauer and E Fuchs
Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA.
Desmosomes mediate intercellular adhesion through desmosomal cadherins, which interface with plakoglobin (PG) and desmoplakin (DP) to associate with the intermediate filament (IF) cytoskeleton. Desmosomes first assemble in the E3.5 mouse trophectoderm, concomitant with establishment of epithelial polarity and appearance of a blastocoel cavity. Increasing in size and number, desmosomes continue their prominence in extra-embryonic tissues, but as development proceeds, they also become abundant in a number of embryonic tissues, including heart muscle, epidermis and neuroepithelium. Previously, we explored the functional importance of desmosomes by ablating the Dsp gene. Homozygous Dsp mutant embryos progressed through implantation, but did not survive beyond E6.5, owing to a loss or instability of desmosomes and tissue integrity. We have now rescued the extra-embryonic tissues by aggregation of tetraploid (wild-type) and diploid (Dsp mutant) morulae. These animals survive several days longer, but die shortly after gastrulation, with major defects in the heart muscle, neuroepithelium and skin epithelium, all of which possess desmosomes, as well as the microvasculature, which does not. Interestingly, although wild-type endothelial cells of capillaries do not form desmosomes, they possess unusual intercellular junctions composed of DP, PG and VE-cadherin. The severity in phenotype and the breadth of defects in the Dsp mutant embryo is greater than PG mutant embryos, substantiating redundancy between PG and other armadillo proteins (e.g. beta-catenin). The timing of lethality is similar to that of the VE-cadherin null embryo, suggesting that a participating cause of death may be a defect in vasculature, not reported for PG null embryos.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  W. W. Franke Discovering the Molecular Components of Intercellular Junctions--A Historical View Cold Spring Harb Perspect Biol, September 1, 2009; 1(3): a003061 - a003061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Delva, D. K. Tucker, and A. P. Kowalczyk The Desmosome Cold Spring Harb Perspect Biol, August 1, 2009; 1(2): a002543 - a002543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. Watson, C. Geary-Joo, M. Hughes, and J. C. Cross The Mrj co-chaperone mediates keratin turnover and prevents the formation of toxic inclusion bodies in trophoblast cells of the placenta Development, May 1, 2007; 134(9): 1809 - 1817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Shasby Cell-cell adhesion in lung endothelium Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L593 - L607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Lorch, T. O. Thomas, and H.-J. Schmoll Bortezomib Inhibits Cell-Cell Adhesion and Cell Migration and Enhances Epidermal Growth Factor Receptor Inhibitor-Induced Cell Death in Squamous Cell Cancer Cancer Res., January 15, 2007; 67(2): 727 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lapouge, L. Fontao, M.-F. Champliaud, F. Jaunin, M. A. Frias, B. Favre, D. Paulin, K. J. Green, and L. Borradori New insights into the molecular basis of desmoplakinand desmin-related cardiomyopathies J. Cell Sci., December 1, 2006; 119(23): 4974 - 4985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Yang, N. E. Bowles, S. E. Scherer, M. D. Taylor, D. L. Kearney, S. Ge, V. V. Nadvoretskiy, G. DeFreitas, B. Carabello, L. I. Brandon, et al. Desmosomal Dysfunction due to Mutations in Desmoplakin Causes Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy Circ. Res., September 15, 2006; 99(6): 646 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Kottke, E. Delva, and A. P. Kowalczyk The desmosome: cell science lessons from human diseases. J. Cell Sci., March 1, 2006; 119(Pt 5): 797 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Den, X. Cheng, M. Merched-Sauvage, and P. J. Koch Desmocollin 3 is required for pre-implantation development of the mouse embryo J. Cell Sci., February 1, 2006; 119(3): 482 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Godsel, S. N. Hsieh, E. V. Amargo, A. E. Bass, L. T. Pascoe-McGillicuddy, A. C. Huen, M. E. Thorne, C. A. Gaudry, J. K. Park, K. Myung, et al. Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin J. Cell Biol., December 19, 2005; 171(6): 1045 - 1059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Xiao, J. Garner, K. M. Buckley, P. A. Vincent, C. M. Chiasson, E. Dejana, V. Faundez, and A. P. Kowalczyk p120-Catenin Regulates Clathrin-dependent Endocytosis of VE-Cadherin Mol. Biol. Cell, November 1, 2005; 16(11): 5141 - 5151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sawa, S.-i. Kuroshima, Y. Yamaoka, and S. Yoshida Intracellular Distribution of Desmoplakin in Human Odontoblasts J. Histochem. Cytochem., September 1, 2005; 53(9): 1099 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-I. Jang, A. Kalinin, K. Takahashi, L. N. Marekov, and P. M. Steinert Characterization of human epiplakin: RNAi-mediated epiplakin depletion leads to the disruption of keratin and vimentin IF networks J. Cell Sci., February 15, 2005; 118(4): 781 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Grossmann, C. Grund, J. Huelsken, M. Behrend, B. Erdmann, W. W. Franke, and W. Birchmeier Requirement of plakophilin 2 for heart morphogenesis and cardiac junction formation J. Cell Biol., October 11, 2004; 167(1): 149 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aho, K. Li, Y. Ryoo, C. McGee, A. Ishida-Yamamoto, J. Uitto, and J. F. Klement Periplakin Gene Targeting Reveals a Constituent of the Cornified Cell Envelope Dispensable for Normal Mouse Development Mol. Cell. Biol., July 15, 2004; 24(14): 6410 - 6418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bazzoni and E. Dejana Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis Physiol Rev, July 1, 2004; 84(3): 869 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhou, A. Stuart, L. E. Dettin, G. Rodriguez, B. Hoel, and G. I. Gallicano Desmoplakin is required for microvascular tube formation in culture J. Cell Sci., July 1, 2004; 117(15): 3129 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Vincent, K. Xiao, K. M. Buckley, and A. P. Kowalczyk VE-cadherin: adhesion at arm's length Am J Physiol Cell Physiol, May 1, 2004; 286(5): C987 - C997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Cheng, K. Mihindukulasuriya, Z. Den, A. P. Kowalczyk, C. C. Calkins, A. Ishiko, A. Shimizu, and P. J. Koch Assessment of Splice Variant-Specific Functions of Desmocollin 1 in the Skin Mol. Cell. Biol., January 1, 2004; 24(1): 154 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Xiao, D. F. Allison, K. M. Buckley, M. D. Kottke, P. A. Vincent, V. Faundez, and A. P. Kowalczyk Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells J. Cell Biol., November 10, 2003; 163(3): 535 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cattelino, S. Liebner, R. Gallini, A. Zanetti, G. Balconi, A. Corsi, P. Bianco, H. Wolburg, R. Moore, B. Oreda, et al. The conditional inactivation of the {beta}-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility J. Cell Biol., September 15, 2003; 162(6): 1111 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fontao, B. Favre, S. Riou, D. Geerts, F. Jaunin, J.-H. Saurat, K. J. Green, A. Sonnenberg, and L. Borradori Interaction of the Bullous Pemphigoid Antigen 1 (BP230) and Desmoplakin with Intermediate Filaments Is Mediated by Distinct Sequences within Their COOH Terminus Mol. Biol. Cell, May 1, 2003; 14(5): 1978 - 1992. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Calkins, B. L. Hoepner, C. M. Law, M. R. Novak, S. V. Setzer, M. Hatzfeld, and A. P. Kowalczyk The Armadillo Family Protein p0071 Is a VE-cadherin- and Desmoplakin-binding Protein J. Biol. Chem., January 10, 2003; 278(3): 1774 - 1783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Huen, J. K. Park, L. M. Godsel, X. Chen, L. J. Bannon, E. V. Amargo, T. Y. Hudson, A. K. Mongiu, I. M. Leigh, D. P. Kelsell, et al. Intermediate filament-membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength J. Cell Biol., December 23, 2002; 159(6): 1005 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Venkiteswaran, K. Xiao, S. Summers, C. C. Calkins, P. A. Vincent, K. Pumiglia, and A. P. Kowalczyk Regulation of endothelial barrier function and growth by VE-cadherin, plakoglobin, and beta -catenin Am J Physiol Cell Physiol, September 1, 2002; 283(3): C811 - C821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Maatta, T. DiColandrea, K. Groot, and F. M. Watt Gene Targeting of Envoplakin, a Cytoskeletal Linker Protein and Precursor of the Epidermal Cornified Envelope Mol. Cell. Biol., October 15, 2001; 21(20): 7047 - 7053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Shasby, D. R. Ries, S. S. Shasby, and M. C. Winter Histamine stimulates phosphorylation of adherens junction proteins and alters their link to vimentin Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1330 - L1338. [Abstract] [Full Text] [PDF]
|
|
