|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 128, Issue 6 965-972, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
R Dorfman and BZ Shilo
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
The BMP pathway patterns the dorsal region of the Drosophila embryo. Using an antibody recognizing phosphorylated Mad (pMad), we followed signaling directly. In wild-type embryos, a biphasic activation pattern is observed. At the cellular blastoderm stage high pMad levels are detected only in the dorsal-most cell rows that give rise to amnioserosa. This accumulation of pMad requires the ligand Screw (Scw), the Short gastrulation (Sog) protein, and cleavage of their complex by Tolloid (Tld). When the inhibitory activity of Sog is removed, Mad phosphorylation is expanded. In spite of the uniform expression of Scw, pMad expansion is restricted to the dorsal domain of the embryo where Dpp is expressed. This demonstrates that Mad phosphorylation requires simultaneous activation by Scw and Dpp. Indeed, the early pMad pattern is abolished when either the Scw receptor Saxophone (Sax), the Dpp receptor Thickveins (Tkv), or Dpp are removed. After germ band extension, a uniform accumulation of pMad is observed in the entire dorsal domain of the embryo, with a sharp border at the junction with the neuroectoderm. From this stage onward, activation by Scw is no longer required, and Dpp suffices to induce high levels of pMad. In these subsequent phases pMad accumulates normally in the presence of ectopic Sog, in contrast to the early phase, indicating that Sog is only capable of blocking activation by Scw and not by Dpp.
This article has been cited by other articles:
|
|
 |
|
  J. Jaeger, D. Irons, and N. Monk Regulative feedback in pattern formation: towards a general relativistic theory of positional information Development, October 1, 2008; 135(19): 3175 - 3183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-c. Lin, J. Park, N. Kirov, and C. Rushlow Threshold response of C15 to the Dpp gradient in Drosophila is established by the cumulative effect of Smad and Zen activators and negative cues Development, December 15, 2006; 133(24): 4805 - 4813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. v. d. Zee, O. Stockhammer, C. v. Levetzow, R. N. d. Fonseca, and S. Roth Sog/Chordin is required for ventral-to-dorsal Dpp/BMP transport and head formation in a short germ insect PNAS, October 31, 2006; 103(44): 16307 - 16312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-C. Yao, I. L. Blitz, D. A. Peiffer, S. Phin, Y. Wang, S. Ogata, K. W. Y. Cho, K. Arora, and R. Warrior Schnurri transcription factors from Drosophila and vertebrates can mediate Bmp signaling through a phylogenetically conserved mechanism Development, October 15, 2006; 133(20): 4025 - 4034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gao and A. Laughon Decapentaplegic-responsive Silencers Contain Overlapping Mad-binding Sites J. Biol. Chem., September 1, 2006; 281(35): 25781 - 25790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. O'Connor, D. Umulis, H. G. Othmer, and S. S. Blair Shaping BMP morphogen gradients in the Drosophila embryo and pupal wing Development, January 15, 2006; 133(2): 183 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Sutherland, M. Li, X.-q. Liu, R. Stefancsik, and L. A. Raftery Stepwise formation of a SMAD activity gradient during dorsal-ventral patterning of the Drosophila embryo Development, December 1, 2003; 130(23): 5705 - 5716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Shimmi and M. B. O'Connor Physical properties of Tld, Sog, Tsg and Dpp protein interactions are predicted to help create a sharp boundary in Bmp signals during dorsoventral patterning of the Drosophila embryo Development, October 1, 2003; 130(19): 4673 - 4682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Akiyama-Oda and H. Oda Early patterning of the spider embryo: a cluster of mesenchymal cells at the cumulus produces Dpp signals received by germ disc epithelial cells Development, May 1, 2003; 130(9): 1735 - 1747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Rusten, R. Cantera, F. C. Kafatos, and R. Barrio The role of TGF{beta} signaling in the formation of the dorsal nervous system is conserved between Drosophila and chordates Development, August 1, 2002; 129(15): 3575 - 3584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dorfman, L. Glazer, U. Weihe, M. F. Wernet, and B.-Z. Shilo Elbow and Noc define a family of zinc finger proteins controlling morphogenesis of specific tracheal branches Development, August 1, 2002; 129(15): 3585 - 3596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ying, X. Qi, and G.-Q. Zhao Induction of primordial germ cells from murine epiblasts by synergistic action of BMP4 and BMP8B signaling pathways PNAS, June 20, 2001; (2001) 151242798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ying, X. Qi, and G.-Q. Zhao Induction of primordial germ cells from murine epiblasts by synergistic action of BMP4 and BMP8B signaling pathways PNAS, July 3, 2001; 98(14): 7858 - 7862. [Abstract] [Full Text] [PDF]
|
|
