spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koizumi, K.
Right arrow Articles by Koseki, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koizumi, K.
Right arrow Articles by Koseki, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 128, Issue 8 1391-1402, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

The role of presenilin 1 during somite segmentation

Ki Koizumi, M Nakajima, S Yuasa, Y Saga, T Sakai, T Kuriyama, T Shirasawa and H Koseki
Department of Molecular Embryology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan. koseki@med.m.chiba-u.ac.jp

The Notch signalling pathway plays essential roles during the specification of the rostral and caudal somite halves and subsequent segmentation of the paraxial mesoderm. We have re-investigated the role of presenilin 1 (Ps1; encoded by Psen1) during segmentation using newly generated alleles of the Psen1 mutation. In Psen1-deficient mice, proteolytic activation of Notch1 was significantly affected and the expression of several genes involved in the Notch signalling pathway was altered, including &Dgr;-like3, Hes5, lunatic fringe (Lfng) and Mesp2. Thus, Ps1-dependent activation of the Notch pathway is essential for caudal half somite development. We observed defects in Notch signalling in both the caudal and rostral region of the presomitic mesoderm. In the caudal presomitic mesoderm, Ps1 was involved in maintaining the amplitude of cyclic activation of the Notch pathway, as represented by significant reduction of Lfng expression in Psen1-deficient mice. In the rostral presomitic mesoderm, rapid downregulation of the Mesp2 expression in the presumptive caudal half somite depends on Ps1 and is a prerequisite for caudal somite half specification. Chimaera analysis between Psen1-deficient and wild-type cells revealed that condensation of the wild-type cells in the caudal half somite was concordant with the formation of segment boundaries, while mutant and wild-type cells intermingled in the presomitic mesoderm. This implies that periodic activation of the Notch pathway in the presomitic mesoderm is still latent to segregate the presumptive rostral and caudal somite. A transient episode of Mesp2 expression might be needed for Notch activation by Ps1 to confer rostral or caudal properties. In summary, we propose that Ps1 is involved in the functional manifestation of the segmentation clock in the presomitic mesoderm.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
M. Morimoto, N. Sasaki, M. Oginuma, M. Kiso, K. Igarashi, K.-i. Aizaki, J. Kanno, and Y. Saga
The negative regulation of Mesp2 by mouse Ripply2 is required to establish the rostro-caudal patterning within a somite
Development, April 15, 2007; 134(8): 1561 - 1569.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Yasuhiko, S. Haraguchi, S. Kitajima, Y. Takahashi, J. Kanno, and Y. Saga
Tbx6-mediated Notch signaling controls somite-specific Mesp2 expression
PNAS, March 7, 2006; 103(10): 3651 - 3656.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Takahashi, S. Kitajima, T. Inoue, J. Kanno, and Y. Saga
Differential contributions of Mesp1 and Mesp2 to the epithelialization and rostro-caudal patterning of somites
Development, February 15, 2005; 132(4): 787 - 796.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Takahashi, T. Inoue, A. Gossler, and Y. Saga
Feedback loops comprising Dll1, Dll3 and Mesp2, and differential involvement of Psen1 are essential for rostrocaudal patterning of somites
Development, September 15, 2003; 130(18): 4259 - 4268.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Yamada, Y. Mizutani-Koseki, T. Hasegawa, N. Osumi, H. Koseki, and N. Takahashi
Cell-autonomous involvement of Mab21l1 is essential for lens placode development
Development, May 1, 2003; 130(9): 1759 - 1770.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Nomura-Kitabayashi, Y. Takahashi, S. Kitajima, T. Inoue, H. Takeda, and Y. Saga
Hypomorphic Mesp allele distinguishes establishment of rostrocaudal polarity and segment border formation in somitogenesis
Development, March 7, 2003; 129(10): 2473 - 2481.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. A. Henry, M. K. Urban, K. K. Dill, J. P. Merlie, M. F. Page, C. B. Kimmel, and S. L. Amacher
Two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, function together to refine alternating somite boundaries
Development, August 1, 2002; 129(15): 3693 - 3704.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Bessho, R. Sakata, S. Komatsu, K. Shiota, S. Yamada, and R. Kageyama
Dynamic expression and essential functions of Hes7 in somite segmentation
Genes & Dev., October 15, 2001; 15(20): 2642 - 2647.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001