|
|
|
|||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||

1 Department of Molecular Biosciences,
2 Cell Therapy Program and
3 ARC SRC for the Molecular Genetics of Development, The University of Adelaide, Adelaide, South Australia 5005, Australia
* Present address: Section of Gene Function and Regulation, Chester Beatty Laboratories, Institute of Cancer Research, London SW3 6JB, UK
Author for correspondence (e-mail: peter.rathjen{at}adelaide.edu.au)
Accepted 8 March 2002
During embryogenesis the central and peripheral nervous systems arise from a neural precursor population, neurectoderm, formed during gastrulation. We demonstrate the differentiation of mouse embryonic stem cells to neurectoderm in culture, in a manner which recapitulates embryogenesis, with the sequential and homogeneous formation of primitive ectoderm, neural plate and neural tube. Formation of neurectoderm occurs in the absence of extraembryonic endoderm or mesoderm and results in a stratified epithelium of cells with morphology, gene expression and differentiation potential consistent with positionally unspecified neural tube. Differentiation of this population to homogeneous populations of neural crest or glia was also achieved. Neurectoderm formation in culture allows elucidation of signals involved in neural specification and generation of implantable cell populations for therapeutic use.
Key words: Stem cells, Neurectoderm, Cell culture, Neural crest
This article has been cited by other articles:
![]() |
B. Coleman, M. G. de Silva, and R. K. Shepherd Concise Review: The Potential of Stem Cells for Auditory Neuron Generation and Replacement Stem Cells, November 1, 2007; 25(11): 2685 - 2694. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Gossrau, J. Thiele, R. Konang, T. Schmandt, and O. Brustle Bone Morphogenetic Protein-Mediated Modulation of Lineage Diversification During Neural Differentiation of Embryonic Stem Cells Stem Cells, April 1, 2007; 25(4): 939 - 949. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Motohashi, H. Aoki, K. Chiba, N. Yoshimura, and T. Kunisada Multipotent Cell Fate of Neural Crest-Like Cells Derived from Embryonic Stem Cells Stem Cells, February 1, 2007; 25(2): 402 - 410. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Shin, M. Mitalipova, S. Noggle, D. Tibbitts, A. Venable, R. Rao, and S. L. Stice Long-Term Proliferation of Human Embryonic Stem Cell-Derived Neuroepithelial Cells Using Defined Adherent Culture Conditions Stem Cells, January 1, 2006; 24(1): 125 - 138. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. M. BAUGHMAN and N. GEIJSEN In Vitro Generation of Germ Cells: New Techniques to Solve Current Issues Ann. N.Y. Acad. Sci., December 1, 2005; 1061(1): 33 - 40. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. C. Brodie and H. D. Humes Stem Cell Approaches for the Treatment of Renal Failure Pharmacol. Rev., September 1, 2005; 57(3): 299 - 313. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. M. Wobus and K. R. Boheler Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy Physiol Rev, April 1, 2005; 85(2): 635 - 678. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. C. Schulz, S. A. Noggle, G. M. Palmarini, D. A. Weiler, I. G. Lyons, K. A. Pensa, A. C.B. Meedeniya, B. P. Davidson, N. A. Lambert, and B. G. Condie Differentiation of Human Embryonic Stem Cells to Dopaminergic Neurons in Serum-Free Suspension Culture Stem Cells, December 1, 2004; 22(7): 1218 - 1238. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. S. O'Shea Self-renewal vs. Differentiation of Mouse Embryonic Stem Cells Biol Reprod, December 1, 2004; 71(6): 1755 - 1765. [Abstract] [Full Text] [PDF] |
||||
![]() |
I. Hiratani, A. Leskovar, and D. M. Gilbert Differentiation-induced replication-timing changes are restricted to AT-rich/long interspersed nuclear element (LINE)-rich isochores PNAS, November 30, 2004; 101(48): 16861 - 16866. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. P. L. Tam and J. Rossant Mouse embryonic chimeras: tools for studying mammalian development Development, December 22, 2003; 130(25): 6155 - 6163. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Rathjen, J. M. Washington, M. D. Bettess, and P. D. Rathjen Identification of a Biological Activity That Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse Biol Reprod, December 1, 2003; 69(6): 1863 - 1871. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. M. Ward, K. Barrow, A. M. Woods, and P. L. Stern The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency J. Cell Sci., November 15, 2003; 116(22): 4533 - 4542. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Mummery, D. Ward-van Oostwaard, P. Doevendans, R. Spijker, S. van den Brink, R. Hassink, M. van der Heyden, T. Opthof, M. Pera, A. B. de la Riviere, et al. Differentiation of Human Embryonic Stem Cells to Cardiomyocytes: Role of Coculture With Visceral Endoderm-Like Cells Circulation, June 3, 2003; 107(21): 2733 - 2740. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Shimozaki, K. Nakashima, H. Niwa, and T. Taga Involvement of Oct3/4 in the enhancement of neuronal differentiation of ES cells in neurogenesis-inducing cultures Development, June 1, 2003; 130(11): 2505 - 2512. [Abstract] [Full Text] [PDF] |
||||