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Development 129, 3645-3656 (2002)
© 2002 The Company of Biologists Limited

Drumstick is a zinc finger protein that antagonizes Lines to control patterning and morphogenesis of the Drosophila hindgut

Ryan B. Green1, Victor Hatini2, Katherine A. Johansen3, Xue-Jun Liu3,* and Judith A. Lengyel1,3,{dagger}

1 Molecular Biology Institute,
3 Department of Molecular, Cell and Developmental Biology, UCLA, Los Angeles, CA 90095-1606, USA
2 Department of Cell and Developmental Biology, University of Pennsylvania, School of Medicine, 1223 BRB2, 421 Curie Boulevard, Philadelphia, PA 19104-6058, USA
* Present address: The R. W. Johnson Pharmaceutical Research Institute, La Jolla, CA, USA

{dagger}Author for correspondence (e-mail: jlengyel{at}ucla.edu)

Accepted 15 May 2002

Elongation of the Drosophila embryonic hindgut epithelium occurs by a process of oriented cell rearrangement requiring the genes drumstick (drm) and lines (lin). The elongating hindgut becomes subdivided into domains – small intestine, large intestine and rectum – each characterized by a specific pattern of gene expression dependent upon normal drm and lin function. We show that drm encodes an 81 amino acid (10 kDa) zinc finger protein that is a member of the Odd-skipped family. drm expression is localized to the developing midgut-hindgut junction and is required to establish the small intestine, while lin is broadly expressed throughout the gut primordium and represses small intestine fate. lin is epistatic to drm, suggesting a model in which localized expression of drm blocks lin activity, thereby allowing small intestine fate to be established. Further supporting this model, ectopic expression of Drm throughout the hindgut produces a lin phenotype. Biochemical and genetic data indicate that the first conserved zinc finger of Drm is essential for its function. We have thus defined a pathway in which a spatially localized zinc finger protein antagonizes a globally expressed protein, thereby leading to specification of a domain (the small intestine) necessary for oriented cell rearrangement.

Key words: Drosophila melanogaster, Hindgut, Cell rearrangement, Patterning, Zinc finger, Relief of repression




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© The Company of Biologists Ltd 2002