|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
1 Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
2 Department of Developmental Biology, Wenner-Gren Institute, Stockholm University, S-106 91 Stockholm, Sweden
3 Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel
4 Molecular Genetics of Development, Faculty of Health Sciences, Ben-Gurian University of the Neger, Beer-Sheva 84105, Israel
* These authors contributed equally to this manuscript
Authors for correspondence (e-mail: dannyc{at}tauex.tau.ac.il and dsegal{at}post.tau.ac.il)
Accepted 17 June 2002
The COP9 signalosome (CSN) is an essential eight-subunit repressor of light-regulated development in Arabidopsis. This complex has also been identified in animals, though its developmental role remains obscure. CSN subunits have been implicated in various cellular processes, suggesting a possible role for the CSN as an integrator of multiple signaling pathways. In order to elucidate the function of the CSN in animals, a Drosophila model system has previously been established. Gel-filtration analysis with antibodies against CSN subunits 4, 5 and 7 revealed that these proteins act as a complex in Drosophila that is similar in size to the plant and mammalian complexes. Null mutations in either one of two subunits, CSN4 or CSN5, are larval lethal. Successful embryogenesis appears to be a consequence of maternal contribution of the complex. Biochemical analysis indicates that the different subunits are found in both CSN-dependent and CSN-independent forms, and that these forms are differentially affected by the mutations. Phenotypic characterization of these two mutants indicates that they show both shared and unique phenotypes, which suggest specific roles for each subunit. Both mutants have defective oocyte and embryo patterning, and defects in response to DNA damage, while csn5 mutants develop melanotic tumors and csn4 mutants have phenotypes reminiscent of defects in ecdysone signaling.
Key words: COP9 signalosome, Maternal effect, Embryo development, Oogenesis, Drosophila
This article has been cited by other articles:
|
|
 |
|
  E. M. N. Dohmann, M. P. Levesque, E. Isono, M. Schmid, and C. Schwechheimer Auxin Responses in Mutants of the Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome Plant Physiology, July 1, 2008; 147(3): 1369 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Oren-Giladi, O. Krieger, B. A. Edgar, D. A. Chamovitz, and D. Segal Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development. Genes Cells, March 1, 2008; 13(3): 221 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Adler, L. E. Littlepage, M. Lin, T. L.A. Kawahara, D. J. Wong, Z. Werb, and H. Y. Chang CSN5 Isopeptidase Activity Links COP9 Signalosome Activation to Breast Cancer Progression Cancer Res., January 15, 2008; 68(2): 506 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Busch, E. U. Schwier, K. Nahlik, O. Bayram, K. Helmstaedt, O. W. Draht, S. Krappmann, O. Valerius, W. N. Lipscomb, and G. H. Braus An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation PNAS, May 8, 2007; 104(19): 8089 - 8094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Ullah, M. S. Buckley, D. N. Arnosti, and R. W. Henry Retinoblastoma Protein Regulation by the COP9 Signalosome Mol. Biol. Cell, April 1, 2007; 18(4): 1179 - 1186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou, M. R. Tarver, and M. E. Scharf Hexamerin-based regulation of juvenile hormone-dependent gene expression underlies phenotypic plasticity in a social insect Development, February 1, 2007; 134(3): 601 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Harari-Steinberg, R. Cantera, S. Denti, E. Bianchi, E. Oron, D. Segal, and D. A Chamovitz COP9 signalosome subunit 5 (CSN5/Jab1) regulates the development of the Drosophila immune system: effects on Cactus, Dorsal and hematopoiesis. Genes Cells, February 1, 2007; 12(2): 183 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gusmaroli, P. Figueroa, G. Serino, and X. W. Deng Role of the MPN Subunits in COP9 Signalosome Assembly and Activity, and Their Regulatory Interaction with Arabidopsis Cullin3-Based E3 Ligases PLANT CELL, February 1, 2007; 19(2): 564 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Denti, M. E. Fernandez-Sanchez, L. Rogge, and E. Bianchi The COP9 Signalosome Regulates Skp2 Levels and Proliferation of Human Cells J. Biol. Chem., October 27, 2006; 281(43): 32188 - 32196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Hallstrom and J. R. Nevins Jab1 is a specificity factor for E2F1-induced apoptosis Genes & Dev., March 1, 2006; 20(5): 613 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Richardson and W. Zundel The Emerging Role of the COP9 Signalosome in Cancer Mol. Cancer Res., December 1, 2005; 3(12): 645 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Lima, I. Malavazi, M. R. von Zeska Kress Fagundes, M. Savoldi, M. H. S. Goldman, E. Schwier, G. H. Braus, and G. H. Goldman The csnD/csnE Signalosome Genes Are Involved in the Aspergillus nidulans DNA Damage Response Genetics, November 1, 2005; 171(3): 1003 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M.N. Dohmann, C. Kuhnle, and C. Schwechheimer Loss of the CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome Subunit 5 Is Sufficient to Cause the cop/det/fus Mutant Phenotype in Arabidopsis PLANT CELL, July 1, 2005; 17(7): 1967 - 1978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tomoda, J.-y. Kato, E. Tatsumi, T. Takahashi, Y. Matsuo, and N. Yoneda-Kato The Jab1/COP9 signalosome subcomplex is a downstream mediator of Bcr-Abl kinase activity and facilitates cell-cycle progression Blood, January 15, 2005; 105(2): 775 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gusmaroli, S. Feng, and X. W. Deng The Arabidopsis CSN5A and CSN5B Subunits Are Present in Distinct COP9 Signalosome Complexes, and Mutations in Their JAMM Domains Exhibit Differential Dominant Negative Effects on Development PLANT CELL, November 1, 2004; 16(11): 2984 - 3001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tomoda, N. Yoneda-Kato, A. Fukumoto, S. Yamanaka, and J.-y. Kato Multiple Functions of Jab1 Are Required for Early Embryonic Development and Growth Potential in Mice J. Biol. Chem., October 8, 2004; 279(41): 43013 - 43018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lykke-Andersen, L. Schaefer, S. Menon, X.-W. Deng, J. B. Miller, and N. Wei Disruption of the COP9 Signalosome Csn2 Subunit in Mice Causes Deficient Cell Proliferation, Accumulation of p53 and Cyclin E, and Early Embryonic Death Mol. Cell. Biol., October 1, 2003; 23(19): 6790 - 6797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yan, K. Walz, H. Nakamura, S. Carattini-Rivera, Q. Zhao, H. Vogel, N. Wei, M. J. Justice, A. Bradley, and J. R. Lupski COP9 Signalosome Subunit 3 Is Essential for Maintenance of Cell Proliferation in the Mouse Embryonic Epiblast Mol. Cell. Biol., October 1, 2003; 23(19): 6798 - 6808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Chamovitz and A. Yahalom A Systems Approach to the COP9 Signalosome Plant Physiology, June 1, 2003; 132(2): 426 - 427. [Full Text] [PDF]
|
|
