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Genetic and molecular characterization of a novel iab-8 regulatory domain in the Abdominal-B gene of Drosophila melanogaster

Beatriz Estrada^*, Fernando Casares, Ana Busturia and Ernesto Sánchez-Herrero

Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
^* Present address: Division of Genetics, HHMI, Brigham and Women's Hospital, 20 Shattuck Street, Boston MA 02115, USA
Present address: Instituto de Biologia Molecular e Celular (IBMC), Rua do Campo Alegre, 823, 4150-180 Porto, Portugal

Author for correspondence (e-mail: esherrero{at}cbm.uam.es)

Accepted 6 August 2002

Homeotic (or Hox) genes are key determinants in specifying the anteroposterior axis of most animals. The temporal and spatial expression of these genes requires the presence of large and complex cis-regulatory regions. The Abdominal-B Hox gene of the bithorax complex of Drosophila is regulated by several infraabdominal domains, which determine Abdominal-B expression in abdominal segments A5 to A9 (parasegments 10 to 14). Some of the infraabdominal domains have been characterized, including an infraabdominal-8 domain, which has been located 3' to the Abdominal-B transcription unit. We have analyzed the expression and mutant phenotype of a P-lacZ element inserted close to the Abdominal-B m origin of transcription and of derivatives of this transposon. Some of these derivatives represent a particular class of mutations in the bithorax complex, because they transform the eighth and ninth abdominal segments without affecting more anterior metameres. The analysis of these mutations and of transformants carrying sequences upstream the Abdominal-B m transcription unit has allowed us to define a new infraabdominal-8 regulatory region, located 5' to the Abdominal-B transcription unit, and has helped to characterize better the complex regulation of the Abdominal-B gene.

Key words: Drosophila, Hox, Abdominal-B, iab regulatory domains