|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00110
DEVELOPMENT AND DISEASE |
1 Department of Life Science, Kwangju Institute of Science and Technology,
Gwangju 500-712, Korea
2 Department of Orthopaedic Surgery, Wonkwang University School of Medicine,
Iksan 570-711, Korea
3 Laboratory of Developmental Biology and Genomics, College of Veterinary
Medicine, Seoul National University, Seoul, Korea
4 TG Biotech, Kyungpook National University, Daegu 702-701, Korea
Author for correspondence (e-mail: jschun{at}kjist.ac.kr)
Accepted 14 August 2002
ß-Catenin regulates important biological processes, including embryonic development and tumorigenesis. We have investigated the role of ß-catenin in the regulation of the chondrocyte phenotype. Expression of ß-catenin was high in prechondrogenic mesenchymal cells, but significantly decreased in differentiated chondrocytes both in vivo and in vitro. Accumulation of ß-catenin by the inhibition of glycogen synthase kinase-3ß with LiCl inhibited chondrogenesis by stabilizing cell-cell adhesion. Conversely, the low level of ß-catenin in differentiated articular chondrocytes was increased by post-translational stabilization during phenotypic loss caused by a serial monolayer culture or exposure to retinoic acid or interleukin-1ß. Ectopic expression of ß-catenin or inhibition of ß-catenin degradation with LiCl or proteasome inhibitor caused de-differentiation of chondrocytes. Transcriptional activation of ß-catenin by its nuclear translocation was sufficient to cause phenotypic loss of differentiated chondrocytes. Expression pattern of Jun, a known target gene of ß-catenin, is essentially the same as that of ß-catenin both in vivo and in vitro suggesting that Jun and possibly activator protein 1 is involved in the ß-catenin regulation of the chondrocyte phenotype.
Key words: Cartilage, Chondrocytes, Differentiation, De-differentiation, ß-Catenin, Chick
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J.-S. Kim, Z. Y. Ryoo, and J.-S. Chun Cytokine-like 1 (CYTL1) Regulates the Chondrogenesis of Mesenchymal Cells J. Biol. Chem., October 5, 2007; 282(40): 29359 - 29367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. H. Huh, J.-H. Ryu, and J.-S. Chun Regulation of Type II Collagen Expression by Histone Deacetylase in Articular Chondrocytes J. Biol. Chem., June 8, 2007; 282(23): 17123 - 17131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Ryu and J.-S. Chun Opposing Roles of WNT-5A and WNT-11 in Interleukin-1beta Regulation of Type II Collagen Expression in Articular Chondrocytes J. Biol. Chem., August 4, 2006; 281(31): 22039 - 22047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wu and F. De Luca Inhibition of the Proteasomal Function in Chondrocytes Down-Regulates Growth Plate Chondrogenesis and Longitudinal Bone Growth Endocrinology, August 1, 2006; 147(8): 3761 - 3768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-G. Hwang, S.-S. Yu, H. Poo, and J.-S. Chun c-Jun/Activator Protein-1 Mediates Interleukin-1{beta}-induced Dedifferentiation but Not Cyclooxygenase-2 Expression in Articular Chondrocytes J. Biol. Chem., August 19, 2005; 280(33): 29780 - 29787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-G. Hwang, S.-S. Yu, J.-H. Ryu, H.-B. Jeon, Y.-J. Yoo, S.-H. Eom, and J.-S. Chun Regulation of {beta}-Catenin Signaling and Maintenance of Chondrocyte Differentiation by Ubiquitin-independent Proteasomal Degradation of {alpha}-Catenin J. Biol. Chem., April 1, 2005; 280(13): 12758 - 12765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Guo, T. F. Day, X. Jiang, L. Garrett-Beal, L. Topol, and Y. Yang Wnt/{beta}-catenin signaling is sufficient and necessary for synovial joint formation Genes & Dev., October 1, 2004; 18(19): 2404 - 2417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-G. Hwang, J.-H. Ryu, I.-C. Kim, E.-H. Jho, H.-C. Jung, K. Kim, S.-J. Kim, and J.-S. Chun Wnt-7a Causes Loss of Differentiated Phenotype and Inhibits Apoptosis of Articular Chondrocytes via Different Mechanisms J. Biol. Chem., June 18, 2004; 279(25): 26597 - 26604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Akiyama, J. P. Lyons, Y. Mori-Akiyama, X. Yang, R. Zhang, Z. Zhang, J. M. Deng, M. M. Taketo, T. Nakamura, R. R. Behringer, et al. Interactions between Sox9 and {beta}-catenin control chondrocyte differentiation Genes & Dev., May 1, 2004; 18(9): 1072 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-J. Kim, S.-G. Hwang, I.-C. Kim, and J.-S. Chun Actin Cytoskeletal Architecture Regulates Nitric Oxide-induced Apoptosis, Dedifferentiation, and Cyclooxygenase-2 Expression in Articular Chondrocytes via Mitogen-activated Protein Kinase and Protein Kinase C Pathways J. Biol. Chem., October 24, 2003; 278(43): 42448 - 42456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-D. Oh and J.-S. Chun Signaling Mechanisms Leading to the Regulation of Differentiation and Apoptosis of Articular Chondrocytes by Insulin-like Growth Factor-1 J. Biol. Chem., September 19, 2003; 278(38): 36563 - 36571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Topol, X. Jiang, H. Choi, L. Garrett-Beal, P. J. Carolan, and Y. Yang Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent {beta}-catenin degradation J. Cell Biol., September 1, 2003; 162(5): 899 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-B. Yoon, S.-J. Kim, S.-G. Hwang, S. Chang, S.-S. Kang, and J.-S. Chun Non-steroidal Anti-inflammatory Drugs Inhibit Nitric Oxide-induced Apoptosis and Dedifferentiation of Articular Chondrocytes Independent of Cyclooxygenase Activity J. Biol. Chem., April 18, 2003; 278(17): 15319 - 15325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Huh, S.-H. Kim, S.-J. Kim, and J.-S. Chun Differentiation Status-dependent Regulation of Cyclooxygenase-2 Expression and Prostaglandin E2 Production by Epidermal Growth Factor via Mitogen-activated Protein Kinase in Articular Chondrocytes J. Biol. Chem., March 7, 2003; 278(11): 9691 - 9697. [Abstract] [Full Text] [PDF]
|
|
