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doi: 10.1242/10.1242/dev.00167

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Development 129, 5659-5665 (2002)
Copyright © 2002 The Company of Biologists Limited

The Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration

Alejandro Sánchez Alvarado*,{dagger},§, Phillip A. Newmark*,{ddagger}, Sofia M. C. Robb{dagger} and Réjeanne Juste

Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210, USA
{dagger} Present address: University of Utah School of Medicine, Department of Neurobiology and Anatomy, Salt Lake City, UT 84132-3401, USA
{ddagger} Present address: University of Illinois at Urbana-Champaign, Department of Cell and Structural Biology, Urbana-Champaign, IL 61801, USA

§ Author for correspondence (e-mail: sanchez{at}neuro.utah.edu)

Accepted 11 August 2002

Platyhelminthes are excellent models for the study of stem cell biology, regeneration and the regulation of scale and proportion. In addition, parasitic forms infect millions of people worldwide. Therefore, it is puzzling that they remain relatively unexplored at the molecular level. We present the characterization of ~3000 non-redundant cDNAs from a clonal line of the planarian Schmidtea mediterranea. The obtained cDNA sequences, homology comparisons and high-throughput whole-mount in situ hybridization data form part of the S. mediterranea database (SmedDb; http://planaria.neuro.utah.edu). Sixty-nine percent of the cDNAs analyzed share similarities with sequences deposited in GenBank and dbEST. The remaining gene transcripts failed to match sequences in other organisms, even though a large number of these (~80%) contained putative open reading frames. Taken together, the molecular resources presented in this study, along with the ability of abrogating gene expression in planarians using RNA interference technology, pave the way for a systematic study of the remarkable biological properties displayed by Platyhelminthes.

Key words: Regeneration, Stem cells, Plasticity, Database


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© The Company of Biologists Ltd 2002