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Differential interactions of eyeless and twin of eyeless with the sine oculis enhancer

Claudio Punzo^*, Makiko Seimiya^*, Susanne Flister, Walter J. Gehring and Serge Plaza

Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
^* The first two authors contributed equally to the work
Present address: Universite Paul Sabatier Centre de Biologie de Developpement, bar 4R3, 118 route de Narbonne, 31062 Toulouse cedex 4, France

Author for correspondence (e-mail: Walter.Gehring{at}unibas.ch)

Accepted 12 October 2001

Drosophila eye development is under the control of early eye specifying genes including eyeless (ey), twin of eyeless (toy), eyes absent (eya), dachshund (dac) and sine oculis (so). They are all conserved between vertebrates and insects and they interact in a combinatorial and hierarchical network to regulate each other expression. so has been shown to be directly regulated by ey through an eye-specific enhancer (so10). We further studied the regulation of this element and found that both Drosophila Pax6 proteins namely EY and TOY bind and positively regulate so10 expression through different binding sites. By targeted mutagenesis experiments, we disrupted these EY and TOY binding sites and studied their functional involvement in the so10 enhancer expression in the eye progenitor cells. We show a differential requirement for the EY and TOY binding sites in activating so10 during the different stages of eye development. Additionally, in a rescue experiment performed in the so¹ mutant, we show that the EY and TOY binding sites are required for compound eye and ocellus development respectively. Altogether, these results suggest a differential requirement for EY and TOY to specify the development of the two types of adult visual systems, namely the compound eye and the ocellus.

Key words: so enhancer, ey, toy, Drosophila, Eye development

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