|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
1 Program in Molecular Cell Biology, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO 63110, USA
2 Department of Genetics, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO 63110, USA
*Author for correspondence (e-mail: jskeath{at}genetics.wustl.edu)
Accepted 13 December 2001
In the Drosophila embryonic central nervous system, neural stem cells, called neuroblasts, acquire fates in a position-specific manner. Recent work has identified a set of genes that functions along the dorsoventral axis to enable neuroblasts that develop in different dorsoventral domains to acquire distinct fates. These genes include the evolutionarily conserved transcription factors ventral nerve cord defective and intermediate neuroblasts defective, as well as the Drosophila EGF receptor. We show that the Sox-domain-containing gene Dichaete/fish-hook also plays a crucial role to pattern the neuroectoderm along the DV axis. Dichaete is expressed in the medial and intermediate columns of the neuroectoderm, and mutant analysis indicates that Dichaete regulates cell fate and neuroblast formation in these domains. Molecular epistasis tests, double mutant analysis and dosage-sensitive interactions demonstrate that during these processes, Dichaete functions in parallel with ventral nerve cord defective and intermediate neuroblasts defective, and downstream of EGF receptor signaling to mediate its effect on development. These results identify Dichaete as an important regulator of dorsoventral pattern in the neuroectoderm, and indicate that Dichaete acts in concert with ventral nerve cord defective and intermediate neuroblasts defective to regulate pattern and cell fate in the neuroectoderm.
Key words: Drosophila, Sox-domain proteins, Dichaete, Neuroectoderm
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K. S Alatzoglou, D. Kelberman, and M. T Dattani The role of SOX proteins in normal pituitary development J. Endocrinol., March 1, 2009; 200(3): 245 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Tran and C. Q. Doe Pdm and Castor close successive temporal identity windows in the NB3-1 lineage Development, November 1, 2008; 135(21): 3491 - 3499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Egger, J. M Chell, and A. H Brand Insights into neural stem cell biology from flies Phil Trans R Soc B, January 12, 2008; 363(1489): 39 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Zhao, G. Boekhoff-Falk, B. A. Wilson, and J. B. Skeath Linking pattern formation to cell-type specification: Dichaete and Ind directly repress achaete gene expression in the Drosophila CNS PNAS, March 6, 2007; 104(10): 3847 - 3852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Urbach, D. Volland, J. Seibert, and G. M. Technau Segment-specific requirements for dorsoventral patterning genes during early brain development in Drosophila Development, November 1, 2006; 133(21): 4315 - 4330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-H. Wang, R. Chmelik, D. Tang, and M. Nirenberg Identification and analysis of vnd/NK-2 homeodomain binding sites in genomic DNA PNAS, May 17, 2005; 102(20): 7097 - 7102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Yu, L.-J. Syu, and D. M. Mellerick Contextual interactions determine whether the Drosophila homeodomain protein, Vnd, acts as a repressor or activator Nucleic Acids Res., January 7, 2005; 33(1): 1 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Moniot, S. Biau, S. Faure, C. M. Nielsen, P. Berta, D. J. Roberts, and P. de Santa Barbara SOX9 specifies the pyloric sphincter epithelium through mesenchymal-epithelial signals Development, August 1, 2004; 131(15): 3795 - 3804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Buescher, F. S. Hing, and W. Chia Formation of neuroblasts in the embryonic central nervous system of Drosophila melanogaster is controlled by SoxNeuro Development, March 11, 2003; 129(18): 4193 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Overton, L. A. Meadows, J. Urban, and S. Russell Evidence for differential and redundant function of the Sox genes Dichaete and SoxN during CNS development in Drosophila Development, March 11, 2003; 129(18): 4219 - 4228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-H. Wang, R. Chmelik, and M. Nirenberg Sequence-specific DNA binding by the vnd/NK-2 homeodomain of Drosophila PNAS, October 1, 2002; 99(20): 12721 - 12726. [Abstract] [Full Text] [PDF]
|
|
