|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Department of Cell Biology and Center for Molecular Neuroscience, C-2210 Medical Center North,Vanderbilt University Medical Center, Nashville, TN 37232-0295, USA
*Author for correspondence (e-mail: kolodzp{at}ctrvax.vanderbilt.edu)
Accepted 27 November 2001
Coordination of F-actin and microtubule dynamics is important for cellular motility and morphogenesis, but little is known about underlying mechanisms. short stop (shot) encodes an evolutionarily conserved, neuronally expressed family of rod-like proteins required for sensory and motor axon extension in Drosophila melanogaster. We identify Shot isoforms that contain N-terminal F-actin and C-terminal microtubule-binding domains, and that crosslink F-actin and microtubules in cultured cells. The F-actin- and microtubule-binding domains of Shot are required in the same molecule for axon extension, though the length of the connecting rod domain can be dramatically reduced without affecting activity. Shot therefore functions as a cytoskeletal crosslinker in axon extension, rather than mediating independent interactions with F-actin and microtubules. A Ca2+-binding motif located adjacent to the microtubule-binding domain is also required for axon extension, suggesting that intracellular Ca2+ release may regulate Shot activity. These results suggest that Shot coordinates regulated interactions between F-actin and microtubules that are crucial for neuronal morphogenesis.
Key words: F-actin, Microtubules, Shot, Plakins, Ca2+, Drosophila
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Gally, F. Wissler, H. Zahreddine, S. Quintin, F. Landmann, and M. Labouesse Myosin II regulation during C. elegans embryonic elongation: LET-502/ROCK, MRCK-1 and PAK-1, three kinases with different roles Development, September 15, 2009; 136(18): 3109 - 3119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sanchez-Soriano, M. Travis, F. Dajas-Bailador, C. Goncalves-Pimentel, A. J. Whitmarsh, and A. Prokop Mouse ACF7 and Drosophila Short stop modulate filopodia formation and microtubule organisation during neuronal growth J. Cell Sci., July 15, 2009; 122(14): 2534 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Alves-Silva, I. Hahn, O. Huber, M. Mende, A. Reissaus, and A. Prokop Prominent Actin Fiber Arrays in Drosophila Tendon Cells Represent Architectural Elements Different from Stress Fibers Mol. Biol. Cell, October 1, 2008; 19(10): 4287 - 4297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. P. G. Van Horck and C. E. Holt A Cytoskeletal Platform for Local Translation in Axons Sci. Signal., February 26, 2008; 1(8): pe11 - pe11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Diogon, F. Wissler, S. Quintin, Y. Nagamatsu, S. Sookhareea, F. Landmann, H. Hutter, N. Vitale, and M. Labouesse The RhoGAP RGA-2 and LET-502/ROCK achieve a balance of actomyosin-dependent forces in C. elegans epidermis to control morphogenesis Development, July 1, 2007; 134(13): 2469 - 2479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lee, M. Nahm, M. Lee, M. Kwon, E. Kim, A. D. Zadeh, H. Cao, H.-J. Kim, Z. H. Lee, S. B. Oh, et al. The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion Development, May 1, 2007; 134(9): 1767 - 1777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Bartolini and G. G. Gundersen Generation of noncentrosomal microtubule arrays J. Cell Sci., October 15, 2006; 119(20): 4155 - 4163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Slep, S. L. Rogers, S. L. Elliott, H. Ohkura, P. A. Kolodziej, and R. D. Vale Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end J. Cell Biol., February 14, 2005; 168(4): 587 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Hasaka, K. A. Myers, and P. W. Baas Role of Actin Filaments in the Axonal Transport of Microtubules J. Neurosci., December 15, 2004; 24(50): 11291 - 11301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lee and J. E. Treisman Excessive Myosin Activity in Mbs Mutants Causes Photoreceptor Movement Out of the Drosophila Eye Disc Epithelium Mol. Biol. Cell, July 1, 2004; 15(7): 3285 - 3295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Fuss, F. Josten, M. Feix, and M. Hoch Cell movements controlled by the Notch signalling cascade during foregut development in Drosophila Development, April 1, 2004; 131(7): 1587 - 1595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Suh, S. F. Oster, S. S. Soehrman, G. Grenningloh, and D. W. Sretavan L1/Laminin Modulation of Growth Cone Response to EphB Triggers Growth Pauses and Regulates the Microtubule Destabilizing Protein SCG10 J. Neurosci., February 25, 2004; 24(8): 1976 - 1986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Bosher, B.-S. Hahn, R. Legouis, S. Sookhareea, R. M. Weimer, A. Gansmuller, A. D. Chisholm, A. M. Rose, J.-L. Bessereau, and M. Labouesse The Caenorhabditis elegans vab-10 spectraplakin isoforms protect the epidermis against internal and external forces J. Cell Biol., May 26, 2003; 161(4): 757 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Reuter, T. M. Nardine, A. Penton, P. Billuart, E. K. Scott, T. Usui, T. Uemura, and L. Luo A mosaic genetic screen for genes necessary for Drosophila mushroom body neuronal morphogenesis Development, March 15, 2003; 130(6): 1203 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Roper, S. L. Gregory, and N. H. Brown The `Spectraplakins': cytoskeletal giants with characteristics of both spectrin and plakin families J. Cell Sci., November 15, 2002; 115(22): 4215 - 4225. [Abstract] [Full Text] [PDF]
|
|
