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Development 129, 1543-1552 (2002)
© 2002 The Company of Biologists Limited

A transcriptional profile of multicellular development in Dictyostelium discoideum

Nancy Van Driessche1,2,*, Chad Shaw1,*, Mariko Katoh6,*, Takahiro Morio6, Richard Sucgang3, Miroslava Ibarra1, Hidekazu Kuwayama6, Tamao Saito7, Hideko Urushihara6, Mineko Maeda8, Ikuo Takeuchi9, Hiroshi Ochiai7, William Eaton5, Jeffrey Tollett1,4, John Halter5, Adam Kuspa1,2,3, Yoshimasa Tanaka6 and Gad Shaulsky1,2,{ddagger}

1 Department of Molecular and Human Genetics,
2 Graduate Program in Developmental Biology,
3 Department of Biochemistry and Molecular Biology,
4 DNA Array Core Facility,
5 Department of PM&R and Division of Neuroscience, Baylor College of Medicine, Houston TX 77030, USA
6 Institute of Biological Sciences, University of Tsukuba, Tsukuba, Japan
7 Division of Biological Sciences, Hokkaido University, Sapporo, Japan
8 Department of Biology, Osaka University, Osaka, Japan
9 Novartis Foundation for the Promotion of Science, Takarazuka, Japan
* These authors contributed equally to this work

{ddagger}Author for correspondence (e-mail: gadi{at}bcm.tmc.edu)

Accepted 9 January 2002

A distinct feature of development in the simple eukaryote Dictyostelium discoideum is an aggregative transition from a unicellular to a multicellular phase. Using genome-wide transcriptional analysis we show that this transition is accompanied by a dramatic change in the expression of more than 25% of the genes in the genome. We also show that the transcription patterns of these genes are not sensitive to the strain or the nutritional history, indicating that Dictyostelium development is a robust physiological process that is accompanied by stereotypical transcriptional events. Analysis of the two differentiated cell types, spores and stalk cells, and their precursors revealed a large number of differentially expressed genes as well as unexpected patterns of gene expression, which shed new light on the timing and possible mechanisms of cell-type divergence. Our findings provide new perspectives on the complexity of the developmental program and the fraction of the genome that is regulated during development.

Supplemental data available on-line

Key words: DNA microarray, Gene expression, Functional genomics, Dictyostelium discoideum




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© The Company of Biologists Ltd 2002