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doi: 10.1242/10.1242/dev.00180

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A single cdk inhibitor, p27^Xic1, functions beyond cell cycle regulation to promote muscle differentiation in Xenopus

Department of Oncology, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2XY, UK

^* Author for correspondence (e-mail: ap113{at}hermes.cam.ac.uk)

Accepted 28 September 2002

The molecular basis of the antagonism between cellular proliferation and differentiation is poorly understood. We have investigated the role of the cyclin-dependent kinase inhibitor p27^Xic1 in the co-ordination of cell cycle exit and differentiation during early myogenesis in vivo using Xenopus embryos. In this report, we demonstrate that p27^Xic1 is highly expressed in the developing myotome, that ablation of p27^Xic1 protein prevents muscle differentiation and that p27^Xic1 synergizes with the transcription factor MyoD to promote muscle differentiation. Furthermore, the ability of p27^Xic1 to promote myogenesis resides in an N-terminal domain and is separable from its cell cycle regulation function. This data demonstrates that a single cyclin-dependent kinase inhibitor, p27^Xic1, controls in vivo muscle differentiation in Xenopus and that regulation of this process by p27^Xic1 requires activities beyond cell cycle inhibition.

Key words: Cell cycle, Cdk inhibitor, Muscle, Xenopus

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