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doi: 10.1242/10.1242/dev.00193
Department of Oncology, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Cambridge CB2 2XY, UK
* Author for correspondence (e-mail: ap113{at}hermes.cam.ac.uk)
Accepted 8 October 2002
We have investigated the role of the cyclin-dependent kinase inhibitor, p27Xic1, in the coordination of cell cycle exit and differentiation during early neurogenesis. We demonstrate that p27Xic1 is highly expressed in cells destined to become primary neurones and is essential for an early stage of neurogenesis. Ablation of p27Xic1 protein prevents differentiation of primary neurones, while overexpressing p27Xic1 promotes their formation. p27Xic1 may enhance neurogenesis by stabilising the bHLH protein, neurogenin. Moreover, the ability of p27Xic1 to stabilise neurogenin and enhance neurogenesis localises to an N-terminal domain of the molecule and is separable from its ability to inhibit the cell cycle.
Key words: Cell cycle, Cdk inhibitor, Neurone, Differentiation, p27Xic1, Xenopus
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