spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

doi: 10.1242/10.1242/dev.00469

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ogura, K.-i.
Right arrow Articles by Kohara, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ogura, K.-i.
Right arrow Articles by Kohara, Y.
Development 130, 2495-2503 (2003)
Copyright © 2003 The Company of Biologists Limited

Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans

Ken-ichi Ogura1,*, Norihito Kishimoto1, Shohei Mitani2, Keiko Gengyo-Ando2 and Yuji Kohara1,{dagger}

1 Genome Biology Laboratory, Center for Genetic Resource Information, National Institute of Genetics, Mishima 411-8540, Japan
2 Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan
* Present address: Department of Pharmacology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan

{dagger} Author for correspondence (e-mail: ykohara{at}lab.nig.ac.jp)

Accepted 11 March 2003

The translation of maternal glp-1 mRNAs is regulated temporally and spatially in C. elegans embryos. The 3' UTR (untranslated region) of the maternal glp-1 mRNA is important for both kinds of regulation. The spatial control region is required to suppress translation in the posterior blastomeres. The temporal one is required to suppress translation in oocytes and one-cell stage embryos. We show that a CCCH zinc-finger protein, POS-1, represses glp-1 mRNA translation by binding to the spatial control region. We identified an RNP-type RNA-binding protein, SPN-4, as a POS-1-interacting protein. SPN-4 is present developmentally from the oocyte to the early embryo and its distribution overlaps with that of POS-1 in the cytoplasm and P granules of the posterior blastomeres. SPN-4 binds to a subregion of the temporal control region in the 3' UTR and is required for the translation of glp-1 mRNA in the anterior blastomeres. We propose that the balance between POS-1 and SPN-4 controls the translation of maternal glp-1 mRNA.

Key words: Caenorhabditis elegans, Maternal mRNA, Translational control, RNA-binding protein, 3' UTR


Related articles in Development:

Spatially controlling translation

Development 2003 130: 1106. [Full Text]  



This article has been cited by other articles:


Home page
 DevelopmentHome page
Y. Budirahardja and P. Gonczy
PLK-1 asymmetry contributes to asynchronous cell division of C. elegans embryos
Development, April 1, 2008; 135(7): 1303 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. D. Page, S. J. Diede, J. R. Tenlen, and E. L. Ferguson
EEL-1, a Hect E3 ubiquitin ligase, controls asymmetry and persistence of the SKN-1 transcription factor in the early C. elegans embryo
Development, June 15, 2007; 134(12): 2303 - 2314.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Pagano, B. M. Farley, L. M. McCoig, and S. P. Ryder
Molecular Basis of RNA Recognition by the Embryonic Polarity Determinant MEX-5
J. Biol. Chem., March 23, 2007; 282(12): 8883 - 8894.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Paterou, P. Walrad, P. Craddy, K. Fenn, and K. Matthews
Identification and Stage-specific Association with the Translational Apparatus of TbZFP3, a CCCH Protein That Promotes Trypanosome Life-cycle Development
J. Biol. Chem., December 22, 2006; 281(51): 39002 - 39013.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. R. Tenlen, J. A. Schisa, S. J. Diede, and B. D. Page
Reduced Dosage of pos-1 Suppresses Mex Mutants and Reveals Complex Interactions Among CCCH Zinc-Finger Proteins During Caenorhabditis elegans Embryogenesis
Genetics, December 1, 2006; 174(4): 1933 - 1945.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
W. Chi and V. Reinke
Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1 (E2F) does not require LIN-35 (pRB)
Development, August 15, 2006; 133(16): 3147 - 3157.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
O. V. Skabkina, D. N. Lyabin, M. A. Skabkin, and L. P. Ovchinnikov
YB-1 Autoregulates Translation of Its Own mRNA at or prior to the Step of 40S Ribosomal Subunit Joining
Mol. Cell. Biol., April 15, 2005; 25(8): 3317 - 3323.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Ciosk, M. DePalma, and J. R. Priess
ATX-2, the C. elegans ortholog of ataxin 2, functions in translational regulation in the germline
Development, October 1, 2004; 131(19): 4831 - 4841.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
G. Kao, S. Tuck, D. Baillie, and M. V. Sundaram
C. elegans SUR-6/PR55 cooperates with LET-92/protein phosphatase 2A and promotes Raf activity independently of inhibitory Akt phosphorylation sites
Development, February 15, 2004; 131(4): 755 - 765.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003