spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

doi: 10.1242/10.1242/dev.00486

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marin, V. A.
Right arrow Articles by Evans, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marin, V. A.
Right arrow Articles by Evans, T. C.
Development 130, 2623-2632 (2003)
Copyright © 2003 The Company of Biologists Limited

Translational repression of a C. elegans Notch mRNA by the STAR/KH domain protein GLD-1

Veronica A. Marin1 and Thomas C. Evans1,2,*

1 Program in Molecular Biology
2 Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, 4200 E. 9th Avenue, Denver, CO 80262, USA

* Author for correspondence (e-mail: tom.evans{at}uchsc.edu)

Accepted 28 February 2003

In C. elegans, the Notch receptor GLP-1 is localized within the germline and early embryo by translational control of glp-1 mRNA. RNA elements in the glp-1 3'untranslated region (3' UTR) are necessary for repression of glp-1 translation in germ cells, and for localization of translation to anterior cells of the early embryo. The direct regulators of glp-1 mRNA are not known. Here, we show that a 34 nucleotide region of the glp-1 3' UTR contains two regulatory elements, an element that represses translation in germ cells and posterior cells of the early embryo, and an element that inhibits repressor activity to promote translation in the embryo. Furthermore, we show that the STAR/KH domain protein GLD-1 binds directly and specifically to the repressor element. Depletion of GLD-1 activity by RNA interference causes loss of endogenous glp-1 mRNA repression in early meiotic germ cells, and in posterior cells of the early embryo. Therefore, GLD-1 is a direct repressor of glp-1 translation at two developmental stages. These results suggest a new function for GLD-1 in regulating early embryonic asymmetry. Furthermore, these observations indicate that precise control of GLD-1 activity by other regulatory factors is important to localize this Notch receptor, and contributes to the spatial organization of Notch signaling.

Key words: Translation, Notch, STAR, GLD-1, Caenorhabdtis elegans




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
S. L. Noble, B. L. Allen, L. K. Goh, K. Nordick, and T. C. Evans
Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development
J. Cell Biol., August 11, 2008; 182(3): 559 - 572.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. D. Page, S. J. Diede, J. R. Tenlen, and E. L. Ferguson
EEL-1, a Hect E3 ubiquitin ligase, controls asymmetry and persistence of the SKN-1 transcription factor in the early C. elegans embryo
Development, June 15, 2007; 134(12): 2303 - 2314.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. R. Tenlen, J. A. Schisa, S. J. Diede, and B. D. Page
Reduced Dosage of pos-1 Suppresses Mex Mutants and Reveals Complex Interactions Among CCCH Zinc-Finger Proteins During Caenorhabditis elegans Embryogenesis
Genetics, December 1, 2006; 174(4): 1933 - 1945.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. P. Paronetto, F. Zalfa, F. Botti, R. Geremia, C. Bagni, and C. Sette
The Nuclear RNA-binding Protein Sam68 Translocates to the Cytoplasm and Associates with the Polysomes in Mouse Spermatocytes
Mol. Biol. Cell, January 1, 2006; 17(1): 14 - 24.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
V. E. Vought, M. Ohmachi, M.-H. Lee, and E. M. Maine
EGO-1, a Putative RNA-Directed RNA Polymerase, Promotes Germline Proliferation in Parallel With GLP-1/Notch Signaling and Regulates the Spatial Organization of Nuclear Pore Complexes and Germline P Granules in Caenorhabditis elegans
Genetics, July 1, 2005; 170(3): 1121 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. M. Maine, D. Hansen, D. Springer, and V. E. Vought
Caenorhabditis elegans atx-2 Promotes Germline Proliferation and the Oocyte Fate
Genetics, October 1, 2004; 168(2): 817 - 830.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Ciosk, M. DePalma, and J. R. Priess
ATX-2, the C. elegans ortholog of ataxin 2, functions in translational regulation in the germline
Development, October 1, 2004; 131(19): 4831 - 4841.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. P. RYDER and J. R. WILLIAMSON
Specificity of the STAR/GSG domain protein Qk1: Implications for the regulation of myelination
RNA, September 1, 2004; 10(9): 1449 - 1458.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. R. Eckmann, S. L. Crittenden, N. Suh, and J. Kimble
GLD-3 and Control of the Mitosis/Meiosis Decision in the Germline of Caenorhabditis elegans
Genetics, September 1, 2004; 168(1): 147 - 160.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M.-H. Lee and T. Schedl
Translation repression by GLD-1 protects its mRNA targets from nonsense-mediated mRNA decay in C. elegans
Genes & Dev., May 1, 2004; 18(9): 1047 - 1059.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. Currie
Developmental diorama
Development, September 1, 2003; 130(17): 3903 - 3906.
[Full Text] [PDF]


© The Company of Biologists Ltd 2003