spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

doi: 10.1242/10.1242/dev.00513

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ayyar, S.
Right arrow Articles by White, R. A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ayyar, S.
Right arrow Articles by White, R. A. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Development 130, 2841-2852 (2003)
Copyright © 2003 The Company of Biologists Limited

Drosophila TGIF is essential for developmentally regulated transcription in spermatogenesis

Savita Ayyar1, Jianqiao Jiang2, Anna Collu1, Helen White-Cooper2 and Robert A. H. White1,*

1 Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK
2 Department of Zoology, Oxford University, South Parks Rd, Oxford, OX1 3PS, UK

* Author for correspondence (e-mail: rw108{at}cam.ac.uk)

Accepted 27 March 2003

We have investigated the role of TGIF, a TALE-class homeodomain transcription factor, in Drosophila development. In vertebrates, TGIF has been implicated, by in vitro analysis, in several pathways, most notably as a repressor modulating the response to TGFß signalling. Human TGIF has been associated with the developmental disorder holoprosencephaly. Drosophila TGIF is represented by the products of two tandemly repeated highly similar genes, achintya and vismay. We have generated mutations that delete both genes. Homozygous mutant flies are viable and appear morphologically normal, but the males are completely sterile. The defect lies at the primary spermatocyte stage and differentiation is blocked prior to the onset of the meiotic divisions. We show that mutants lacking TGIF function fail to activate transcription of many genes required for sperm manufacture and of some genes required for entry into the meiotic divisions. This groups TGIF together with two other genes producing similar phenotypes, always early and cookie monster, as components of the machinery required for the activation of the spermatogenic programme of transcription. TGIF is the first sequence-specific transcription factor identified in this pathway. By immunolabelling in mouse testes we show that TGIF is expressed in the early stages of spermatogenesis consistent with a conserved role in the activation of the spermatogenesis transcription programme.

Key words: Drosophila development, Spermatogenesis, Homeodomain, Spermatocyte


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
T. M. Franklin-Dumont, C. Chatterjee, S. A. Wasserman, and S. DiNardo
A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes
Development, August 1, 2007; 134(15): 2851 - 2861.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Jiang, E. Benson, N. Bausek, K. Doggett, and H. White-Cooper
Tombola, a tesmin/TSO1-family protein, regulates transcriptional activation in the Drosophila male germline and physically interacts with Always early
Development, April 15, 2007; 134(8): 1549 - 1559.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. M. Mikhaylova, A. M. Boutanaev, and D. I. Nurminsky
Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line
PNAS, August 8, 2006; 103(32): 11975 - 11980.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Mar and P. A. Hoodless
Embryonic fibroblasts from mice lacking tgif were defective in cell cycling.
Mol. Cell. Biol., June 1, 2006; 26(11): 4302 - 4310.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Leonard, D. Rendic, C. Rabouille, I. B. H. Wilson, T. Preat, and F. Altmann
The Drosophila fused lobes Gene Encodes an N-Acetylglucosaminidase Involved in N-Glycan Processing
J. Biol. Chem., February 24, 2006; 281(8): 4867 - 4875.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Shen and C. A. Walsh
Targeted Disruption of Tgif, the Mouse Ortholog of a Human Holoprosencephaly Gene, Does Not Result in Holoprosencephaly in Mice
Mol. Cell. Biol., May 1, 2005; 25(9): 3639 - 3647.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Hiller, X. Chen, M. J. Pringle, M. Suchorolski, Y. Sancak, S. Viswanathan, B. Bolival, T.-Y. Lin, S. Marino, and M. T. Fuller
Testis-specific TAF homologs collaborate to control a tissue-specific transcription program
Development, November 1, 2004; 131(21): 5297 - 5308.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L. Perezgasga, J. Jiang, B. Bolival Jr, M. Hiller, E. Benson, M. T. Fuller, and H. White-Cooper
Regulation of transcription of meiotic cell cycle and terminal differentiation genes by the testis-specific Zn-finger protein matotopetli
Development, April 15, 2004; 131(8): 1691 - 1702.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003