|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00450
1 Department of Molecular Cell Biology, Max-Planck-Institute of Biophysical
Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany
2 Department of Developmental Biology, Max-Planck-Institute of Biophysical
Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany
* Present address: The Salk Institute for Biological Studies, Gene Expression
Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Present address: Sackler Faculty of Medicine, Department of Human Genetics and
Molecular Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv,
Israel
Author for correspondence (e-mail:
peter.gruss{at}mpg-gv.mpg.de)
Accepted 12 February 2003
The transcription factors Pax2 and Pax6 are co-expressed in the entire optic vesicle (OV) prior and concomitant with the establishment of distinct neuroretinal, retinal, pigmented-epithelial and optic-stalk progenitor domains, suggesting redundant functions during retinal determination. Pax2; Pax6 compound mutants display a dose-dependent reduction in the expression of the melanocyte determinant Mitf, accompanied by transdifferentiation of retinal pigmented epithelium (RPE) into neuroretina (NR) in Pax2-/-; Pax6+/- embryos, which strongly resembles the phenotype of Mitf-null mutants. In Pax2-/-; Pax6-/- OVs Mitf fails to be expressed and NR markers occupy the area that usually represents the Mitf+ RPE domain. Furthermore, both, Pax2 and Pax6 bind to and activate a MITF RPE-promoter element in vitro, whereas prolonged expression of Pax6 in the Pax2-positive optic stalk leads to ectopic Mitf expression and RPE differentiation in vivo. Together, these results demonstrate that the redundant activities of Pax2 and Pax6 direct the determination of RPE, potentially by directly controlling the expression of RPE determinants.
Key words: Eye development, Pigmented retina, Regionalization, Pax2, Pax6, Mitf
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Vopalensky and Z. Kozmik Eye evolution: common use and independent recruitment of genetic components Phil Trans R Soc B, October 12, 2009; 364(1531): 2819 - 2832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Osakada, Z.-B. Jin, Y. Hirami, H. Ikeda, T. Danjyo, K. Watanabe, Y. Sasai, and M. Takahashi In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction J. Cell Sci., September 1, 2009; 122(17): 3169 - 3179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Oron-Karni, C. Farhy, M. Elgart, T. Marquardt, L. Remizova, O. Yaron, Q. Xie, A. Cvekl, and R. Ashery-Padan Dual requirement for Pax6 in retinal progenitor cells Development, December 15, 2008; 135(24): 4037 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-A. Mao, S. W. Wang, P. Pan, and W. H. Klein Rewiring the retinal ganglion cell gene regulatory network: Neurod1 promotes retinal ganglion cell fate in the absence of Math5 Development, October 15, 2008; 135(20): 3379 - 3388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bharti, W. Liu, T. Csermely, S. Bertuzzi, and H. Arnheiter Alternative promoter use in eye development: the complex role and regulation of the transcription factor MITF Development, March 15, 2008; 135(6): 1169 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Xu, P. D. Witmer, S. Lumayag, B. Kovacs, and D. Valle MicroRNA (miRNA) Transcriptome of Mouse Retina and Identification of a Sensory Organ-specific miRNA Cluster J. Biol. Chem., August 24, 2007; 282(34): 25053 - 25066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Li, D. Goldowitz, and D. J. Swanson The Requirement of Pax6 for Postnatal Eye Development: Evidence from Experimental Mouse Chimeras Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3292 - 3300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Evans and P. J. Gage Expression of the homeobox gene Pitx2 in neural crest is required for optic stalk and ocular anterior segment development Hum. Mol. Genet., November 15, 2005; 14(22): 3347 - 3359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ikeda, F. Osakada, K. Watanabe, K. Mizuseki, T. Haraguchi, H. Miyoshi, D. Kamiya, Y. Honda, N. Sasai, N. Yoshimura, et al. Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells PNAS, August 9, 2005; 102(32): 11331 - 11336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Mui, J. W. Kim, G. Lemke, and S. Bertuzzi Vax genes ventralize the embryonic eye Genes & Dev., May 15, 2005; 19(10): 1249 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Azuma, K. Tadokoro, A. Asaka, M. Yamada, Y. Yamaguchi, H. Handa, S. Matsushima, T. Watanabe, Y. Kida, T. Ogura, et al. Transdifferentiation of the retinal pigment epithelia to the neural retina by transfer of the Pax6 transcriptional factor Hum. Mol. Genet., April 15, 2005; 14(8): 1059 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Horsford, M.-T. T. Nguyen, G. C. Sellar, R. Kothary, H. Arnheiter, and R. R. McInnes Chx10 repression of Mitf is required for the maintenance of mammalian neuroretinal identity Development, January 1, 2005; 132(1): 177 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rowan, C.-M. A. Chen, T. L. Young, D. E. Fisher, and C. L. Cepko Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10 Development, October 15, 2004; 131(20): 5139 - 5152. [Abstract] [Full Text] [PDF]
|
|
