QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

doi: 10.1242/10.1242/dev.00517

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klinedinst, S. L. Articles by Bodmer, R. Search for Related Content PubMed PubMed Citation Articles by Klinedinst, S. L. Articles by Bodmer, R.

Gata factor Pannier is required to establish competence for heart progenitor formation

Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA

^* Author for correspondence (e-mail: rolf{at}umich.edu)

Accepted 31 March 2003

Inductive signaling is of pivotal importance for developmental patterns to form. In Drosophila, the transfer of TGFß (Dpp) and Wnt (Wg) signaling information from the ectoderm to the underlying mesoderm induces cardiac-specific differentiation in the presence of Tinman, a mesoderm-specific homeobox transcription factor. We present evidence that the Gata transcription factor, Pannier, and its binding partner U-shaped, also a zinc-finger protein, cooperate in the process of heart development. Loss-of-function and germ layer-specific rescue experiments suggest that pannier provides an essential function in the mesoderm for initiation of cardiac-specific expression of tinman and for specification of the heart primordium. u-shaped also promotes heart development, but unlike pannier, only by maintaining tinman expression in the cardiogenic region. By contrast, pan-mesodermal overexpression of pannier ectopically expands tinman expression, whereas overexpression of u-shaped inhibits cardiogenesis. Both factors are also required for maintaining dpp expression after germ band retraction in the dorsal ectoderm. Thus, we propose that Pannier mediates as well as maintains the cardiogenic Dpp signal. In support, we find that manipulation of pannier activity in either germ layer affects cardiac specification, suggesting that its function is required in both the mesoderm and the ectoderm.

Key words: Drosophila, Heart, Cardiogenesis, Mesoderm, pannier, u-shaped, tinman, dpp, Gata factors

Related articles in Development:

Flying to the heart of heart development

Development 2003 130: 1304. [Full Text]  

This article has been cited by other articles:

				A. N. Johnson, L. A. Burnett, J. Sellin, A. Paululat, and S. J. Newfeld Defective Decapentaplegic Signaling Results in Heart Overgrowth and Reduced Cardiac Output in Drosophila Genetics, July 1, 2007; 176(3): 1609 - 1624. [Abstract] [Full Text] [PDF]

				T. Akasaka, S. Klinedinst, K. Ocorr, E. L. Bustamante, S. K. Kim, and R. Bodmer The ATP-sensitive potassium (KATP) channel-encoded dSUR gene is required for Drosophila heart function and is regulated by tinman PNAS, August 8, 2006; 103(32): 11999 - 12004. [Abstract] [Full Text] [PDF]

				Z. Han, P. Yi, X. Li, and E. N. Olson Hand, an evolutionarily conserved bHLH transcription factor required for Drosophila cardiogenesis and hematopoiesis Development, March 15, 2006; 133(6): 1175 - 1182. [Abstract] [Full Text] [PDF]

				M. Fujioka, R. J. Wessells, Z. Han, J. Liu, K. Fitzgerald, G. L. Yusibova, M. Zamora, P. Ruiz-Lozano, R. Bodmer, and J. B. Jaynes Embryonic even skipped-Dependent Muscle and Heart Cell Fates Are Required for Normal Adult Activity, Heart Function, and Lifespan Circ. Res., November 25, 2005; 97(11): 1108 - 1114. [Abstract] [Full Text] [PDF]

				I. Reim and M. Frasch The Dorsocross T-box genes are key components of the regulatory network controlling early cardiogenesis in Drosophila Development, November 15, 2005; 132(22): 4911 - 4925. [Abstract] [Full Text] [PDF]

				A. Singh, J. Chan, J. J. Chern, and K.-W. Choi Genetic Interaction of Lobe With Its Modifiers in Dorsoventral Patterning and Growth of the Drosophila Eye Genetics, September 1, 2005; 171(1): 169 - 183. [Abstract] [Full Text] [PDF]

				Z. Han and E. N. Olson Hand is a direct target of Tinman and GATA factors during Drosophila cardiogenesis and hematopoiesis Development, August 1, 2005; 132(15): 3525 - 3536. [Abstract] [Full Text] [PDF]

				A. Rojas, S. De Val, A. B. Heidt, S.-M. Xu, J. Bristow, and B. L. Black Gata4 expression in lateral mesoderm is downstream of BMP4 and is activated directly by Forkhead and GATA transcription factors through a distal enhancer element Development, August 1, 2005; 132(15): 3405 - 3417. [Abstract] [Full Text] [PDF]

				J. Wang, Y. Tao, I. Reim, K. Gajewski, M. Frasch, and R. A. Schulz Expression, Regulation, and Requirement of the Toll Transmembrane Protein during Dorsal Vessel Formation in Drosophila melanogaster Mol. Cell. Biol., May 15, 2005; 25(10): 4200 - 4210. [Abstract] [Full Text] [PDF]

				K. Tsarovina, A. Pattyn, J. Stubbusch, F. Muller, J. van der Wees, C. Schneider, J.-F. Brunet, and H. Rohrer Essential role of Gata transcription factors in sympathetic neuron development Development, October 1, 2004; 131(19): 4775 - 4786. [Abstract] [Full Text] [PDF]

				M. Zikova, J.-P. Da Ponte, B. Dastugue, and K. Jagla Patterning of the cardiac outflow region in Drosophila PNAS, October 14, 2003; 100(21): 12189 - 12194. [Abstract] [Full Text] [PDF]