|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00549
Botanical Institute III, University of Köln, Gyrhofstrasse 15, Köln, D-50931, Germany
* Authors for correspondence (e mail: jaideep.mathur{at}uni-koeln.de and martin.huelskamp{at}uni-koeln.de)
Accepted 15 April 2003
The generation of a specific cell shape requires differential growth, whereby specific regions of the cell expand more relative to others. The Arabidopsis crooked mutant exhibits aberrant cell shapes that develop because of mis-directed expansion, especially during a rapid growth phase. GFP-aided visualization of the F-actin cytoskeleton and the behavior of subcellular organelles in different cell-types in crooked and wild-type Arabidopsis revealed that localized expansion is promoted in cellular regions with fine F-actin arrays but is restricted in areas that maintain dense F-actin. This suggested that a spatiotemporal distinction between fine versus dense F-actin in a growing cell could determine the final shape of the cell. CROOKED was molecularly identified as the plant homolog of ARPC5, the smallest sub-unit of the ARP2/3 complex that in other organisms is renowned for its role in creating dendritic arrays of fine F-actin. Rescue of crooked phenotype by the human ortholog provides the first molecular evidence for the presence and functional conservation of the complex in higher plants. Our cell-biological and molecular characterization of CROOKED suggests a general actin-based mechanism for regulating differential growth and generating cell shape diversity.
Key words: Cytoskeleton, ARP2/3-complex, Actin, Trichomes, Morphogenesis
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in Development:
This article has been cited by other articles:
|
|
 |
|
  M. D. Marks, J. P. Wenger, E. Gilding, R. Jilk, and R. A. Dixon Transcriptome Analysis of Arabidopsis Wild-Type and gl3-sst sim Trichomes Identifies Four Additional Genes Required for Trichome Development Mol Plant, June 19, 2009; (2009) ssp037v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Maisch, J. Fiserova, L. Fischer, and P. Nick Tobacco Arp3 is localized to actin-nucleating sites in vivo J. Exp. Bot., February 1, 2009; 60(2): 603 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yokota, E. Fukai, L. H. Madsen, A. Jurkiewicz, P. Rueda, S. Radutoiu, M. Held, M. S. Hossain, K. Szczyglowski, G. Morieri, et al. Rearrangement of Actin Cytoskeleton Mediates Invasion of Lotus japonicus Roots by Mesorhizobium loti PLANT CELL, January 1, 2009; 21(1): 267 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dyachok, M.-R. Shao, K. Vaughn, A. Bowling, M. Facette, S. Djakovic, L. Clark, and L. Smith Plasma Membrane-Associated SCAR Complex Subunits Promote Cortical F-Actin Accumulation and Normal Growth Characteristics in Arabidopsis Roots Mol Plant, November 1, 2008; 1(6): 990 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhang, E. L. Mallery, J. Schlueter, S. Huang, Y. Fan, S. Brankle, C. J. Staiger, and D. B. Szymanski Arabidopsis SCARs Function Interchangeably to Meet Actin-Related Protein 2/3 Activation Thresholds during Morphogenesis PLANT CELL, April 1, 2008; 20(4): 995 - 1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-F. Perroud and R. S. Quatrano BRICK1 Is Required for Apical Cell Growth in Filaments of the Moss Physcomitrella patens but Not for Gametophore Morphology PLANT CELL, February 1, 2008; 20(2): 411 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guimil and C. Dunand Cell growth and differentiation in Arabidopsis epidermal cells J. Exp. Bot., November 1, 2007; 58(14): 3829 - 3840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Uhrig, M. Mutondo, I. Zimmermann, M. J. Deeks, L. M. Machesky, P. Thomas, S. Uhrig, C. Rambke, P. J. Hussey, and M. Hulskamp The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis Development, March 1, 2007; 134(5): 967 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Djakovic, J. Dyachok, M. Burke, M. J. Frank, and L. G. Smith BRICK1/HSPC300 functions with SCAR and the ARP2/3 complex to regulate epidermal cell shape in Arabidopsis Development, March 15, 2006; 133(6): 1091 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Grennan High Impact Plant Physiology, December 1, 2005; 139(4): 1574 - 1575. [Full Text] [PDF]
|
|
|
|
|
|
P. A. Harries, A. Pan, and R. S. Quatrano Actin-Related Protein2/3 Complex Component ARPC1 Is Required for Proper Cell Morphogenesis and Polarized Cell Growth in Physcomitrella patens PLANT CELL, August 1, 2005; 17(8): 2327 - 2339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, J. Dyachok, S. Krishnakumar, L. G. Smith, and D. G. Oppenheimer IRREGULAR TRICHOME BRANCH1 in Arabidopsis Encodes a Plant Homolog of the Actin-Related Protein2/3 Complex Activator Scar/WAVE That Regulates Actin and Microtubule Organization PLANT CELL, August 1, 2005; 17(8): 2314 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Prassinos, J.-H. Ko, J. Yang, and K.-H. Han Transcriptome Profiling of Vertical Stem Segments Provides Insights into the Genetic Regulation of Secondary Growth in Hybrid Aspen Trees Plant Cell Physiol., August 1, 2005; 46(8): 1213 - 1225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Michelot, C. Guerin, S. Huang, M. Ingouff, S. Richard, N. Rodiuc, C. J. Staiger, and L. Blanchoin The Formin Homology 1 Domain Modulates the Actin Nucleation and Bundling Activity of Arabidopsis FORMIN1 PLANT CELL, August 1, 2005; 17(8): 2296 - 2313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tamura, T. Shimada, M. Kondo, M. Nishimura, and I. Hara-Nishimura KATAMARI1/MURUS3 Is a Novel Golgi Membrane Protein That Is Required for Endomembrane Organization in Arabidopsis PLANT CELL, June 1, 2005; 17(6): 1764 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhong, D. H. Burk, C. J. Nairn, A. Wood-Jones, W. H. Morrison III, and Z.-H. Ye Mutation of SAC1, an Arabidopsis SAC Domain Phosphoinositide Phosphatase, Causes Alterations in Cell Morphogenesis, Cell Wall Synthesis, and Actin Organization PLANT CELL, May 1, 2005; 17(5): 1449 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-B. Li, X.-P. Fan, X.-L. Wang, L. Cai, and W.-C. Yang The Cotton ACTIN1 Gene Is Functionally Expressed in Fibers and Participates in Fiber Elongation PLANT CELL, March 1, 2005; 17(3): 859 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mathur Conservation of boundary extension mechanisms between plants and animals J. Cell Biol., February 28, 2005; 168(5): 679 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Basu, J. Le, S. E.-D. El-Essal, S. Huang, C. Zhang, E. L. Mallery, G. Koliantz, C. J. Staiger, and D. B. Szymanski DISTORTED3/SCAR2 Is a Putative Arabidopsis WAVE Complex Subunit That Activates the Arp2/3 Complex and Is Required for Epidermal Morphogenesis PLANT CELL, February 1, 2005; 17(2): 502 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. O. Wasteneys and Z. Yang New Views on the Plant Cytoskeleton Plant Physiology, December 1, 2004; 136(4): 3884 - 3891. [Full Text] [PDF]
|
|
|
|
|
|
M. B. Sheahan, C. J. Staiger, R. J. Rose, and D. W. McCurdy A Green Fluorescent Protein Fusion to Actin-Binding Domain 2 of Arabidopsis Fimbrin Highlights New Features of a Dynamic Actin Cytoskeleton in Live Plant Cells Plant Physiology, December 1, 2004; 136(4): 3968 - 3978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Frank, C. Egile, J. Dyachok, S. Djakovic, M. Nolasco, R. Li, and L. G. Smith Activation of Arp2/3 complex-dependent actin polymerization by plant proteins distantly related to Scar/WAVE PNAS, November 16, 2004; 101(46): 16379 - 16384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, K. Sorefan, G. Hemmann, and M. W. Bevan Arabidopsis NAP and PIR Regulate Actin-Based Cell Morphogenesis and Multiple Developmental Processes Plant Physiology, November 1, 2004; 136(3): 3616 - 3627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Basu, S. E.-D. El-Assal, J. Le, E. L. Mallery, and D. B. Szymanski Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex subunit SRA1 during leaf epidermal development Development, September 1, 2004; 131(17): 4345 - 4355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Brembu, P. Winge, M. Seem, and A. M. Bones NAPP and PIRP Encode Subunits of a Putative Wave Regulatory Protein Complex Involved in Plant Cell Morphogenesis PLANT CELL, September 1, 2004; 16(9): 2335 - 2349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Saedler, N. Mathur, B. P. Srinivas, B. Kernebeck, M. Hulskamp, and J. Mathur Actin Control Over Microtubules Suggested by DISTORTED2 Encoding the Arabidopsis ARPC2 Subunit Homolog Plant Cell Physiol., July 15, 2004; 45(7): 813 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Cheung and H.-m. Wu Overexpression of an Arabidopsis Formin Stimulates Supernumerary Actin Cable Formation from Pollen Tube Cell Membrane PLANT CELL, January 1, 2004; 16(1): 257 - 269. [Abstract] [Full Text] [PDF]
|
|
