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First published online January 18, 2008
doi: 10.1242/10.1242/dev.00543
1 Department of the Control of Genetic Processes, Institute of Gene Biology,
Russian Academy of Sciences, Moscow 117334, Russia
2 Center for Medical Studies, University of Oslo at the Institute of Gene
Biology, Russian Academy of Sciences, Moscow 117334, Russia
3 Department of Zoology, University of Geneva, CH1211 Geneva, Switzerland
* Present address: Institute de Genetique et de Biologie Moleculaire et
Cellulaire, CNRS/INSERM/ULP, 67404 IIIkirch Cedex, France
These authors contributed equally to the work
Author for correspondence (e-mail:
georgiev_p{at}mail.ru)
Accepted 27 March 2003
The best characterized chromatin insulator in Drosophila is the Suppressor of Hairy wing binding region contained within the gypsy retrotransposon. Although cellular functions have been suggested, no role has been found yet for the multitude of endogenous Suppressor of Hairy wing binding sites. Here we show that two Suppressor of Hairy wing binding sites in the intergenic region between the yellow gene and the Achaete-scute gene complex form a functional insulator. Genetic analysis shows that at least two proteins, Suppressor of Hairy wing and Modifier of MDG4, required for the activity of this insulator, are involved in the transcriptional regulation of Achaete-scute.
Key words: Drosophila melanogaste, Achaete-scute Complex, Insulator, Su(Hw), Mod(mdg4), Enhancer blocking
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