spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

doi: 10.1242/10.1242/dev.00550

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mercier, R.
Right arrow Articles by Franklin, F. C. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mercier, R.
Right arrow Articles by Franklin, F. C. H.
Development 130, 3309-3318 (2003)
Copyright © 2003 The Company of Biologists Limited

The meiotic protein SWI1 is required for axial element formation and recombination initiation in Arabidopsis

Raphael Mercier1,*, Susan. J. Armstrong1, Christine Horlow2, Neil P. Jackson1, Christopher A. Makaroff3, Daniel Vezon2, Georges Pelletier2, Gareth H. Jones1 and F. Christopher H. Franklin1,*

1 School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, UK
2 Institut National de la Recherche Agronomique, Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, 78026 Versailles, cedex, France
3 Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA

* Author for correspondence (e-mail: rmercier{at}versailles.inra.fr and F.C.H.Franklin{at}bham.ac.uk)

Accepted 15 April 2003

We report the detailed characterization of SWITCH1 (SWI1) an Arabidopsis thaliana protein that has been linked with the establishment of sister chromatid cohesion during meiosis. Using a combination of cytological methods including immunolocalization of meiotic chromosome-associated proteins we show that SWI1 is required for formation of axial elements. Our studies reveal that the swi1-2 mutation prevents the formation of RAD51 foci during meiotic prophase and suppresses the chromosome fragmentation phenotype of the recombination-defective dif1-1 mutant. Together, these data suggest that SWI1 may be required for meiotic recombination initiation. Finally we raised an antibody against SWI1 and showed, by immunolocalization coupled with bromodeoxyuridine incorporation experiments, that SWI1 is expressed exclusively in meiotic G1 and S phase. Thus, SWI1 appears to be required for early meiotic events that are at the crossroad of sister chromatid cohesion, recombination and axial element formation. The possible inter-relationship between these processes and the function of SWI1 are discussed.

Key words: Meiosis, Recombination, Synaptonemal complex, Sister chromatid cohesion




This article has been cited by other articles:


Home page
 Mol PlantHome page
K. A. Boateng, X. Yang, F. Dong, H. A. Owen, and C. A. Makaroff
SWI1 Is Required for Meiotic Chromosome Remodeling Events
Mol Plant, July 1, 2008; 1(4): 620 - 633.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
D. Phillips, E. I. Mikhailova, L. Timofejeva, J. L. Mitchell, O. Osina, S. P. Sosnikhina, R. N. Jones, and G. Jenkins
Dissecting Meiosis of Rye using Translational Proteomics
Ann. Bot., April 1, 2008; 101(6): 873 - 880.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
P. E. Stronghill and C. A. Hasenkampf
Analysis of substage associations in prophase I of meiosis in floral buds of wild-type Arabidopsis thaliana (Brassicaceae)
Am. J. Botany, December 1, 2007; 94(12): 2063 - 2067.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
E. Sanchez-Moran, J.-L. Santos, G. H. Jones, and F. C. H. Franklin
ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis
Genes & Dev., September 1, 2007; 21(17): 2220 - 2233.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. S. Hawley
Meiosis in living color: Fluorescence-based tetrad analysis in Arabidopsis
PNAS, March 6, 2007; 104(10): 3673 - 3674.
[Full Text] [PDF]

Home page
 J Exp BotHome page
J. A. da Costa-Nunes, A. M. Bhatt, S. O'Shea, C. E. West, C. M. Bray, U. Grossniklaus, and H. G. Dickinson
Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals differential responses to ionizing radiation
J. Exp. Bot., March 1, 2006; 57(4): 971 - 983.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. J. Wijeratne, C. Chen, W. Zhang, L. Timofejeva, and H. Ma
The Arabidopsis thaliana PARTING DANCERS Gene Encoding a Novel Protein Is Required for Normal Meiotic Homologous Recombination
Mol. Biol. Cell, March 1, 2006; 17(3): 1331 - 1343.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
V. Schubert, M. Klatte, A. Pecinka, A. Meister, Z. Jasencakova, and I. Schubert
Sister Chromatids Are Often Incompletely Aligned in Meristematic and Endopolyploid Interphase Nuclei of Arabidopsis thaliana
Genetics, January 1, 2006; 172(1): 467 - 475.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Chelysheva, S. Diallo, D. Vezon, G. Gendrot, N. Vrielynck, K. Belcram, N. Rocques, A. Marquez-Lema, A. M. Bhatt, C. Horlow, et al.
AtREC8 and AtSCC3 are essential to the monopolar orientation of the kinetochores during meiosis
J. Cell Sci., October 15, 2005; 118(20): 4621 - 4632.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Li, X. Yang, Z. Lin, L. Timofejeva, R. Xiao, C. A. Makaroff, and H. Ma
The AtRAD51C Gene Is Required for Normal Meiotic Chromosome Synapsis and Double-Stranded Break Repair in Arabidopsis
Plant Physiology, June 1, 2005; 138(2): 965 - 976.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J.-I. Itoh, K.-I. Nonomura, K. Ikeda, S. Yamaki, Y. Inukai, H. Yamagishi, H. Kitano, and Y. Nagato
Rice Plant Development: from Zygote to Spikelet
Plant Cell Physiol., January 15, 2005; 46(1): 23 - 47.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
Z. A Wilson and C. Yang
Plant gametogenesis: conservation and contrasts in development
Reproduction, November 1, 2004; 128(5): 483 - 492.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Sanchez-Moran, G. H. Jones, F. C. H. Franklin, and J. L. Santos
A Puromycin-Sensitive Aminopeptidase Is Essential for Meiosis in Arabidopsis thaliana
PLANT CELL, November 1, 2004; 16(11): 2895 - 2909.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. D. Higgins, S. J. Armstrong, F. C. H. Franklin, and G. H. Jones
The Arabidopsis MutS homolog AtMSH4 functions at an early step in recombination: evidence for two classes of recombination in Arabidopsis
Genes & Dev., October 15, 2004; 18(20): 2557 - 2570.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K.-I. Nonomura, M. Nakano, T. Fukuda, M. Eiguchi, A. Miyao, H. Hirochika, and N. Kurata
The Novel Gene HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 of Rice Encodes a Putative Coiled-Coil Protein Required for Homologous Chromosome Pairing in Meiosis
PLANT CELL, April 1, 2004; 16(4): 1008 - 1020.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
H. A. Webber, L. Howard, and S. E. Bickel
The cohesion protein ORD is required for homologue bias during meiotic recombination
J. Cell Biol., March 15, 2004; 164(6): 819 - 829.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. V. Reddy, J. Kaur, B. Agashe, V. Sundaresan, and I. Siddiqi
The DUET gene is necessary for chromosome organization and progression during male meiosis in Arabidopsis and encodes a PHD finger protein
Development, December 15, 2003; 130(24): 5975 - 5987.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003