|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00568
Zoologisches Institut der Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
* Authors for correspondence (e-mail: hafen{at}zool.unizh.ch and nairz{at}zool.unizh.ch)
Accepted 29 April 2003
ERK MAP kinase plays a key role in relaying extracellular signals to transcriptional regulation. As different activity levels or the different duration of ERK activity can elicit distinct responses in one and the same cell, ERK has to be under strict positive and negative control. Although numerous genes acting positively in the ERK signaling pathway have been recovered in genetic screens, mutations in genes encoding negative ERK regulators appear underrepresented. We therefore sought to genetically characterize the dual-specificity phosphatase DMKP3. First, we established a novel assay to elucidate the substrate preferences of eukaryotic phosphatases in vivo and thereby confirmed the specificity of DMKP3 as an ERK phosphatase. The Dmkp3 overexpression phenotype characterized in this assay permitted us to isolate Dmkp3 null mutations. By genetic analysis we show that DMKP3 and the tyrosine phosphatase PTP-ER perform partially redundant functions on the same substrate, ERK. DMKP3 functions autonomously in a subset of photoreceptor progenitor cells in eye imaginal discs. In addition, DMKP3 function appears to be required in surrounding non-neuronal cells for ommatidial patterning and photoreceptor differentiation.
Key words: Drosophila, DMKP3, CL100, Eye dual-specificity phosphatase, Signal transduction
This article has been cited by other articles:
|
|
 |
|
  O. V. Mortensen, M. B. Larsen, B. M. Prasad, and S. G. Amara Genetic Complementation Screen Identifies a Mitogen-activated Protein Kinase Phosphatase, MKP3, as a Regulator of Dopamine Transporter Trafficking Mol. Biol. Cell, July 1, 2008; 19(7): 2818 - 2829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rodriguez, B. Kim, N.-S. Lee, S. Veeranki, and L. Kim MPL1, a Novel Phosphatase with Leucine-Rich Repeats, Is Essential for Proper ERK2 Phosphorylation and Cell Motility Eukaryot. Cell, June 1, 2008; 7(6): 958 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. A. Zeng, M. Rahnama, S. Wang, W. Sosu-Sedzorme, and E. M. Verheyen Drosophila Nemo antagonizes BMP signaling by phosphorylation of Mad and inhibition of its nuclear accumulation Development, June 1, 2007; 134(11): 2061 - 2071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sese, M. Corominas, H. Stocker, T. I. Heino, E. Hafen, and F. Serras The Cdi/TESK1 kinase is required for Sevenless signaling and epithelial organization in the Drosophila eye J. Cell Sci., December 15, 2006; 119(24): 5047 - 5056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cela and M. Llimargas Egfr is essential for maintaining epithelial integrity during tracheal remodelling in Drosophila Development, August 15, 2006; 133(16): 3115 - 3125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. McEwen and M. Peifer Puckered, a Drosophila MAPK phosphatase, ensures cell viability by antagonizing JNK-induced apoptosis Development, September 1, 2005; 132(17): 3935 - 3946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Smith, D. Sweetman, M. Patterson, S. M. Keyse, and A. Munsterberg Feedback interactions between MKP3 and ERK MAP kinase control scleraxis expression and the specification of rib progenitors in the developing chick somite Development, March 15, 2005; 132(6): 1305 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tsang, S. Maegawa, A. Kiang, R. Habas, E. Weinberg, and I. B. Dawid A role for MKP3 in axial patterning of the zebrafish embryo Development, June 15, 2004; 131(12): 2769 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tsang and I. B. Dawid Promotion and Attenuation of FGF Signaling Through the Ras-MAPK Pathway Sci. Signal., April 13, 2004; 2004(228): pe17 - pe17. [Abstract] [Full Text] [PDF]
|
|
