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doi: 10.1242/10.1242/dev.00603
Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Köln, Germany
* Author for correspondence (e-mail: jose.campos{at}uni-koeln.de)
Accepted 14 May 2003
In the neural plate and neural tube in the trunk region of the zebrafish
embryo, dividing cells are oriented parallel to the plane of the
neuroepithelium, while in neural keel/rod, cells divide perpendicular to it.
This change in the orientation of mitosis is brought about by a 90°
rotation of the mitotic spindle. As the two halves of the neural primordium in
keel/rod stage are in apposition, the perpendicular orientation of mitoses in
this stage determines that daughter cells become allocated to both sides of
the neural tube. To assess the role played by cell junctions in controlling
the orientation of dividing cells, we studied the expression of components of
adherens and tight junctions in the neuroepithelial cells. We find that these
proteins are distributed irregularly at the neural plate stage and become
polarised apically in the cell membrane only during the keel/rod stage. The
stereotypic orientation of mitoses is perturbed only weakly upon loss of
function of the cell junction components ASIP and aPKC
, suggesting
that mitotic orientation depends in part on the integrity of cell junctions
and the polarity of the epithelium as a whole. However, the 90° rotation
of the spindle does not require perfectly polarised cell junctions between the
neuroepithelial cells.
Key words: Zebrafish, Neurulation, Mitoses, Orientation, Spindle rotation, Neuroepithelial polarity, ASIP, Histone2A.F/Z:GFP, tau:GFP
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