|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online July 21, 2003
doi: 10.1242/10.1242/dev.00616
The Wellcome Trust/Cancer Research UK Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
Author for correspondence (e-mail:
ds139{at}mole.bio.cam.ac.uk)
Accepted 19 May 2003
The PAR-1 kinase plays a conserved role in cell polarity in C. elegans, Drosophila and mammals. We have investigated the role of PAR-1 in epithelial polarity by generating null mutant clones in the Drosophila follicular epithelium. Large clones show defects in apicobasal membrane polarity, but small clones induced later in development usually have a normal membrane polarity. However, all cells that lack PAR-1 accumulate spectrin and F-actin laterally, and show a strong increase in the density of microtubules. This is consistent with the observation that the mammalian PAR-1 homologues, the MARKs, dramatically reduce the number of microtubules, when overexpressed in tissue culture cells. The MARKs have been proposed to destabilize microtubules by inhibiting the stabilizing activity of the Tau family of microtubule-associated proteins. This is not the case in Drosophila, however, as null mutations in the single tau family member in the genome have no effect on the microtubule organisation in the follicle cells. Furthermore, PAR-1 activity stabilises microtubules, as microtubules in mutant cells depolymerise much more rapidly after cold or colcemid treatments. Loss of PAR-1 also disrupts the basal localisation of the microtubule plus ends, which are mislocalised to the centre of mutant cells. Thus, Drosophila PAR-1 regulates the density, stability and apicobasal organisation of microtubules. Although the direct targets of PAR-1 are unknown, we suggest that it functions by regulating the plus ends, possibly by capping them at the basal cortex.
Key words: PAR-1, Tau, Follicle cells, Drosophila
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Gergely and R. Basto Multiple centrosomes: together they stand, divided they fall Genes & Dev., September 1, 2008; 22(17): 2291 - 2296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Ossipova, J. Tabler, J. B. A. Green, and S. Y. Sokol PAR1 specifies ciliated cells in vertebrate ectoderm downstream of aPKC Development, December 1, 2007; 134(23): 4297 - 4306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kojima, H. Miyoshi, H. C. Clevers, M. Oshima, M. Aoki, and M. M. Taketo Suppression of Tubulin Polymerization by the LKB1-Microtubule-associated Protein/Microtubule Affinity-regulating Kinase Signaling J. Biol. Chem., August 10, 2007; 282(32): 23532 - 23540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Mirouse, L. L. Swick, N. Kazgan, D. St Johnston, and J. E. Brenman LKB1 and AMPK maintain epithelial cell polarity under energetic stress J. Cell Biol., May 7, 2007; 177(3): 387 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Hurov, M. Huang, L. S. White, J. Lennerz, C. S. Choi, Y.-R. Cho, H.-J. Kim, J. L. Prior, D. Piwnica-Worms, L. C. Cantley, et al. Loss of the Par-1b/MARK2 polarity kinase leads to increased metabolic rate, decreased adiposity, and insulin hypersensitivity in vivo PNAS, March 27, 2007; 104(13): 5680 - 5685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Elbert, D. Cohen, and A. Musch PAR1b Promotes Cell-Cell Adhesion and Inhibits Dishevelled-mediated Transformation of Madin-Darby Canine Kidney Cells Mol. Biol. Cell, August 1, 2006; 17(8): 3345 - 3355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Ducharme, C. M. Hales, L. A. Lapierre, A.-J. L. Ham, A. Oztan, G. Apodaca, and J. R. Goldenring MARK2/EMK1/Par-1B{alpha} Phosphorylation of Rab11-Family Interacting Protein 2 Is Necessary for the Timely Establishment of Polarity in Madin-Darby Canine Kidney Cells Mol. Biol. Cell, August 1, 2006; 17(8): 3625 - 3637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cordes, C. A. Frank, and G. Garriga The C. elegans MELK ortholog PIG-1 regulates cell size asymmetry and daughter cell fate in asymmetric neuroblast divisions Development, July 15, 2006; 133(14): 2747 - 2756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bayraktar, D. Zygmunt, and R. W. Carthew Par-1 kinase establishes cell polarity and functions in Notch signaling in the Drosophila embryo J. Cell Sci., February 15, 2006; 119(4): 711 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Jansen, W W J de Leng, A F Baas, H Myoshi, L Mathus-Vliegen, M M Taketo, H Clevers, F M Giardiello, and G J A Offerhaus Mucosal prolapse in the pathogenesis of Peutz-Jeghers polyposis. Gut, January 1, 2006; 55(1): 1 - 5. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Januschke, L. Gervais, L. Gillet, G. Keryer, M. Bornens, and A. Guichet The centrosome-nucleus complex and microtubule organization in the Drosophila oocyte Development, January 1, 2006; 133(1): 129 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Margolis and J.-P. Borg Apicobasal polarity complexes J. Cell Sci., November 15, 2005; 118(22): 5157 - 5159. [Full Text] [PDF]
|
|
|
|
|
|
A. B. da Cruz, M. Schwarzel, S. Schulze, M. Niyyati, M. Heisenberg, and D. Kretzschmar Disruption of the MAP1B-related Protein FUTSCH Leads to Changes in the Neuronal Cytoskeleton, Axonal Transport Defects, and Progressive Neurodegeneration in Drosophila Mol. Biol. Cell, May 1, 2005; 16(5): 2433 - 2442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Elbert, G. Rossi, and P. Brennwald The Yeast Par-1 Homologs Kin1 and Kin2 Show Genetic and Physical Interactions with Components of the Exocytic Machinery Mol. Biol. Cell, February 1, 2005; 16(2): 532 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cohen, E. Rodriguez-Boulan, and A. Musch Par-1 promotes a hepatic mode of apical protein trafficking in MDCK cells PNAS, September 21, 2004; 101(38): 13792 - 13797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cohen, P. J. Brennwald, E. Rodriguez-Boulan, and A. Musch Mammalian PAR-1 determines epithelial lumen polarity by organizing the microtubule cytoskeleton J. Cell Biol., March 1, 2004; 164(5): 717 - 727. [Abstract] [Full Text] [PDF]
|
|
