|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online July 21, 2003
doi: 10.1242/10.1242/dev.00633
1 Howard Hughes Medical Institute and Department of Biological Chemistry,
University of California, Los Angeles, CA 90095-1662, USA
2 University of Pennsylvania, Department of Cell and Developmental Biology, 421
Curie Boulevard, Philadelphia, PA 19104-6058, USA
Author for correspondence (e-mail:
derobert{at}hhmi.ucla.edu)
Accepted 20 May 2003
The determination of the vertebrate dorsoventral body axis is regulated in the extracellular space by a system of interacting secreted molecules consisting of BMP, Chordin, Tolloid and Twisted Gastrulation (Tsg). Tsg is a BMP-binding protein that forms ternary complexes with BMP and Chordin. We investigated the function of Tsg in embryonic patterning by generating point mutations in its two conserved cysteine-rich domains. Surprisingly, Tsg proteins with mutations in the N-terminal domain were unable to bind BMP, yet ventralized the embryo very effectively, indicating strong pro-BMP activity. This hyperventralizing Tsg activity required an intact C-terminal domain and could block the anti-BMP activity of isolated BMP-binding modules of Chordin (CRs) in embryonic assays. This activity was specific for CR-containing proteins as it did not affect the dorsalizing effects of Noggin or dominant-negative BMP receptor. The ventralizing effects of the xTsg mutants were stronger than the effect of Chordin loss-of-function in Xenopus or zebrafish. The results suggest that xTsg interacts with additional CR-containing proteins that regulate dorsoventral development in embryos.
Key words: Twisted-gastrulation, BMP, Chordin, Tolloid, Crossveinless, TGFß, Cell-cell signaling, Xenopus, Zebrafish, CR modules
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Herr, G. Korniychuk, Y. Yamamoto, K. Grubisic, and M. Oelgeschlager Regulation of TGF-{beta} signalling by N-acetylgalactosaminyltransferase-like 1 Development, May 15, 2008; 135(10): 1813 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Zhang, Y. Huang, L.-Y. Qiu, J. Nickel, and W. Sebald von Willebrand Factor Type C Domain-containing Proteins Regulate Bone Morphogenetic Protein Signaling through Different Recognition Mechanisms J. Biol. Chem., July 6, 2007; 282(27): 20002 - 20014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Rentzsch, C. Guder, D. Vocke, B. Hobmayer, and T. W. Holstein An ancient chordin-like gene in organizer formation of Hydra PNAS, February 27, 2007; 104(9): 3249 - 3254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schmidl, N. Adam, C. Surmann-Schmitt, T. Hattori, M. Stock, U. Dietz, B. de Crombrugghe, E. Poschl, and K. von der Mark Twisted Gastrulation Modulates Bone Morphogenetic Protein-induced Collagen II and X Expression in Chondrocytes in Vitro and in Vivo J. Biol. Chem., October 20, 2006; 281(42): 31790 - 31800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rentzsch, J. Zhang, C. Kramer, W. Sebald, and M. Hammerschmidt Crossveinless 2 is an essential positive feedback regulator of Bmp signaling during zebrafish gastrulation Development, March 1, 2006; 133(5): 801 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. O'Connor, D. Umulis, H. G. Othmer, and S. S. Blair Shaping BMP morphogen gradients in the Drosophila embryo and pupal wing Development, January 15, 2006; 133(2): 183 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Gazzerro, V. Deregowski, S. Vaira, and E. Canalis Overexpression of Twisted Gastrulation Inhibits Bone Morphogenetic Protein Action and Prevents Osteoblast Cell Differentiation in Vitro Endocrinology, September 1, 2005; 146(9): 3875 - 3882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zakin, B. Reversade, H. Kuroda, K. M. Lyons, and E. M. De Robertis Sirenomelia in Bmp7 and Tsg compound mutant mice: requirement for Bmp signaling in the development of ventral posterior mesoderm Development, May 15, 2005; 132(10): 2489 - 2499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xie and S. Fisher Twisted gastrulation enhances BMP signaling through chordin dependent and independent mechanisms Development, January 15, 2005; 132(2): 383 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Little and M. C. Mullins Twisted gastrulation promotes BMP signaling in zebrafish dorsal-ventral axial patterning Development, December 1, 2004; 131(23): 5825 - 5835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zakin and E. M. De Robertis Inactivation of mouse Twisted gastrulation reveals its role in promoting Bmp4 activity during forebrain development Development, January 15, 2004; 131(2): 413 - 424. [Abstract] [Full Text] [PDF]
|
|
