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First published online August 18, 2003
doi: 10.1242/10.1242/dev.00664
1 Department of Dermatology and Biomolecular Therapeutics (BMT), University of
Vienna, Medical School, Brunnerstr. 59, A-1235 Vienna, Austria
2 Research Institute of Molecular Pathology (IMP), Dr Bohr-Gasse 7, A-1030
Vienna, Austria
3 Division of Molecular Genetics and Centre of Biomedical Genetics, The
Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands
* Author for correspondence (e-mail: maria.sibilia{at}univie.ac.at)
Accepted 12 June 2003
Mice lacking the epidermal growth factor receptor (EGFR) develop epithelial defects and a neurodegenerative disease and die within the first month of birth. By employing a conditional knock-in approach using the human EGFR cDNA mice humanised for EGFR (hEGFRKI/KI) were generated. Homozygous hEGFRKI/KI mice are viable and live up to six months. However, these mice are growth retarded and show skin and hair defects similar to Egfr-/- mutants. Interestingly, the neurodegeneration is fully rescued in hEGFRKI/KI mice, however, they develop a severe heart hypertrophy with semilunar valve abnormalities. Moreover, hEGFRKI/KI mice display accelerated chondrocyte and osteoblast differentiation, a phenotype that is also present in Egfr-/- mice and has not been previously described. The severity of the phenotypes correlates with the expression levels of the hEGFRKI allele, which is not efficiently expressed in epithelial and bone cells, but is expressed at similar and even higher levels as the endogenous Egfr in brain and heart. These results demonstrate that mice humanised for EGFR display tissue-specific hypomorphic phenotypes and describe a novel function for EGFR in bone development.
Key words: Bone, Hair growth, Heart hypertrophy, Humanised EGFR knock-in mice, Skin
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