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doi: 10.1242/10.1242/dev.00207
1 Section of Cell and Developmental Biology, University of California, San
Diego, 9500 Gilman Drive, La Jolla, CA 92093-0349, USA
2 Albert-Ludwigs-Universität Freiburg, Institut für Biologie I,
Hauptstrasse 1, D-79104 Freiburg, Germany
3 Department of Biology, Dickinson College, PO Box 1773, Carlisle, PA 17013,
USA
4 Max-Planck-Institut für biophysikalische Chemie, Abt. Molekulare
Entwicklungsbiologie, Am Fassberg 11, D-37077 Göttingen, Germany
* Author for correspondence (e-mail: bier{at}biomail.ucsd.edu)
Accepted 3 October 2002
The adjacent knirps (kni) and knirps-related (knrl) genes encode functionally related zinc finger transcription factors that collaborate to initiate development of the second longitudinal wing vein (L2). kni and knrl are expressed in the third instar larval wing disc in a narrow stripe of cells just anterior to the broad central zone of cells expressing high levels of the related spalt genes. Here, we identify a 1.4 kb cis-acting enhancer element from the kni locus that faithfully directs gene expression in the L2 primordium. We find that three independent ri alleles have alterations mapping within the L2-enhancer element and show that two of these observed lesions eliminate the ability of the enhancer element to direct gene expression in the L2 primordium. The L2 enhancer can be subdivided into distinct activation and repression domains. The activation domain mediates the combined action of the general wing activator Scalloped and a putative locally provided factor, the activity of which is abrogated by a single nucleotide alteration in the ri53j mutant. We also find that misexpression of genes in L2 that are normally expressed in veins other than L2 results in abnormal L2 development. These experiments provide a mechanistic basis for understanding how kni and knrl link AP patterning to morphogenesis of the L2 vein by orchestrating the expression of a selective subset of vein-promoting genes in the L2 primordium.
Key words: knirps, radius incompletus, L2 vein, wing, Drosophila melanogaster, Patterning, Morphogenesis
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