|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00213
1 Neurobiotechnology Center and Department of Neuroscience, Ohio State
University, 105 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210,
USA
2 Department of Pathology, Centre Medical Universitaire 1, Rue Michel-Servet,
1211 Geneva 4, Switzerland
* Author for correspondence (e-mail: henion.1{at}osu.edu)
Accepted 8 October 2002
Clonal and lineage analyses have demonstrated that although some neural crest cells have the ability to generate multiple cell types and display self-renewal ability, other crest cells generate a single or limited repertoire of cell types. However, it is not yet clear when, and in what order, crest cells become specified to adopt a particular fate. We report that the receptor tyrosine kinases TrkC and C-Kit are expressed by distinct neural crest subpopulations in vitro. We then analyzed the lineages of individual receptor-expressing crest cells and found that TrkC-expressing cells that have just emerged from the neural tube give rise to clones containing neurons or glial cells, or both, but never produce melanocytes. A short time later, TrkC-expressing cells only generate pure neuronal clones. By contrast, from their earliest appearance in neural tube outgrowths, C-Kit-expressing cells invariably give rise to clones containing only melanocytes. Our results directly demonstrate that distinct neurogenic and melanogenic sublineages diverge before or soon after crest cells emerge from the neural tube, that fate-restricted precursors are present in nascent neural crest populations and that these sublineages can be distinguished by their cell type-specific expression of receptor tyrosine kinases.
Key words: Neural crest, Clonal analysis, Lineage, TrkC, C-Kit, Neurogenic, Melanogenic, Quail
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Motohashi, K. Yamanaka, K. Chiba, H. Aoki, and T. Kunisada Unexpected Multipotency of Melanoblasts Isolated from Murine Skin Stem Cells, April 1, 2009; 27(4): 888 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Harris, R. Hall, and C. A. Erickson Directing pathfinding along the dorsolateral path - the role of EDNRB2 and EphB2 in overcoming inhibition Development, December 15, 2008; 135(24): 4113 - 4122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. McGraw, A. Nechiporuk, and D. W. Raible Zebrafish Dorsal Root Ganglia Neural Precursor Cells Adopt a Glial Fate in the Absence of Neurogenin1 J. Neurosci., November 19, 2008; 28(47): 12558 - 12569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J.L Fernandes, J. G Toma, and F. D Miller Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential Phil Trans R Soc B, January 12, 2008; 363(1489): 185 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Motohashi, H. Aoki, K. Chiba, N. Yoshimura, and T. Kunisada Multipotent Cell Fate of Neural Crest-Like Cells Derived from Embryonic Stem Cells Stem Cells, February 1, 2007; 25(2): 402 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamazaki, M. Tsuneto, M. Yoshino, K.-I. Yamamura, and S.-I. Hayashi Potential of Dental Mesenchymal Cells in Developing Teeth Stem Cells, January 1, 2007; 25(1): 78 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yamazaki, E. Sakata, T. Yamane, A. Yanagisawa, K. Abe, K.-I. Yamamura, S.-I. Hayashi, and T. Kunisada Presence and distribution of neural crest-derived cells in the murine developing thymus and their potential for differentiation Int. Immunol., May 1, 2005; 17(5): 549 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kleber, H.-Y. Lee, H. Wurdak, J. Buchstaller, M. M. Riccomagno, L. M. Ittner, U. Suter, D. J. Epstein, and L. Sommer Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling J. Cell Biol., April 25, 2005; 169(2): 309 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Kasemeier-Kulesa, P. M. Kulesa, and F. Lefcort Imaging neural crest cell dynamics during formation of dorsal root ganglia and sympathetic ganglia Development, January 15, 2005; 132(2): 235 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Wilson, K. L. Richards, M. L. Ford-Perriss, J.-J. Panthier, and M. Murphy Neural crest cell lineage segregation in the mouse neural tube Development, December 15, 2004; 131(24): 6153 - 6162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Planque, G. Raposo, L. Leconte, O. Anezo, P. Martin, and S. Saule Microphthalmia Transcription Factor Induces Both Retinal Pigmented Epithelium and Neural Crest Melanocytes from Neuroretina Cells J. Biol. Chem., October 1, 2004; 279(40): 41911 - 41917. [Abstract] [Full Text] [PDF]
|
|
