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doi: 10.1242/10.1242/dev.00203
DEVELOPMENT AND DISEASE |
1 Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch,
Robert-Rössle-Str. 10, 13125 Berlin, Germany
2 VolkswagenStiftung Research Group, Dept. of Experimental Neurology,
Charité University Hospital, Humboldt University, Schumannstr. 20/21,
10117 Berlin, Germany
3 Department of Psychiatry, Freie Universität Berlin, Eschenallee 3, 14050
Berlin, Germany
4 Department of Physiology, Graduate School of Medicine, University of Tokyo,
Bunkyo-ku, Tokyo 113-0033, Japan
5 The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North
Torrey Pines Rd., La Jolla, CA 92037, USA
* Author for correspondence (e-mail: gerd.kempermann{at}mdc-berlin.de)
Accepted 11 October 2002
New neurons are continually generated in the adult hippocampus, but the important question, whether adult neurogenesis is transient or leads to the lasting presence of new neurons, has not yet been answered. Dividing cells were labeled with bromodeoxyuridine (BrdU) and were investigated by means of immunofluorescence and confocal microscopy at several time-points 1 day to 11 months thereafter. BrdU-labeled neurons remained stable in number and in their relative position in the granule cell layer over at least 11 months. This finding implies that the addition of new neurons is not transient and that their final number and localization are determined early. By contrast, expression of immature markers ß-III-tubulin and doublecortin in BrdU-labeled cells, peaked early after division and was not detectable after 4 weeks. In transgenic mice expressing enhanced green fluorescent protein under the nestin promoter none of the BrdU/nestin-positive cells early after division expressed the mature marker NeuN, confirming that no dividing neurons were detected. These new data suggest that new neurons are recruited early from the pool of proliferating progenitor cells and lead to a lasting effect of adult neurogenesis.
Key words: Stem cell, Progenitor cell, Adult neurogenesis, Mouse
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