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First published online September 2, 2003
doi: 10.1242/10.1242/dev.00701

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Development 130, 4761-4768 (2003)
Copyright © 2003 The Company of Biologists Limited

PRIMER

Investigating C. elegans development through mosaic analysis

John Yochem and Robert K. Herman

Department of Genetics, Cell Biology and Development, University of Minnesota, 6-160 Jackson Hall, 321 Church Street, Minneapolis, MN 55455, USA

SUMMARY

The analysis of genetically mosaic worms, in which some cells carry a wild-type gene and others are homozygous mutant, can reveal where in the animal a gene acts to prevent the appearance of a mutant phenotype. In this primer article, we describe how Caenorhabditis elegans genetic mosaics are generated, identified and analyzed, and we discuss examples in which the analysis of mosaic worms has provided important information about the development of this organism.


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Related articles in Development:

Piecing together genetic mosaics

Development 2003 130: 2001. [Full Text]  



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© The Company of Biologists Ltd 2003