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First published online October 6, 2003
doi: 10.1242/10.1242/dev.00763
1 Department of Organismal Biology and Anatomy, University of Chicago, Chicago,
IL 60637, USA
2 Department of Molecular Biology, Princeton University, Princeton, NJ 08544,
USA
3 Department of Cell Biology and Anatomy, Gautier Building, Room 517, 1011 NW
15th Street, University of Miami School of Medicine, Miami, FL 33136,
USA
Author for correspondence (e-mail:
abruce{at}uchicago.edu)
Accepted 30 July 2003
Early embryonic development in many organisms relies upon maternal molecules deposited into the egg prior to fertilization. We have cloned and characterized a maternal T-box gene in the zebrafish, eomesodermin (eomes). During oogenesis, the eomes transcript becomes localized to the cortex of the oocyte. After fertilization during early cleavage stages, eomes is expressed in a vegetal to animal gradient in the embryo, whereas Eomesodermin protein (Eom) is distributed cytoplasmically throughout the blastoderm. Strikingly, following midblastula transition, nuclear-localized Eomesodermin is detected on the dorsal side of the embryo only. Overexpression of eomes results in Nodal-dependent and nieuwkoid/dharma (nwk/dhm) independent ectopic expression of the organizer markers goosecoid (gsc), chordin (chd) and floating head (flh) and in the formation of secondary axes. The same phenotypes are observed when a VP16-activator construct is injected into early embryos, indicating that eomes acts as a transcriptional activator. In addition, a dominant-negative construct and antisense morpholino oligonucleotides led to a reduction in gsc and flh expression. Together these data indicate that eomes plays a role in specifying the organizer.
Key words: Zebrafish, T-box, eomesodermin, Maternal, VegT, Nodal, squint, bozozok, goosecoid, chordin, floating head, nieuwkoid/dharma
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