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First published online October 22, 2003
doi: 10.1242/10.1242/dev.00805

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Development 130, 5839-5849 (2003)
Copyright © 2003 The Company of Biologists Limited

Maternal mRNAs of PEM and macho 1, the ascidian muscle determinant, associate and move with a rough endoplasmic reticulum network in the egg cortex

Christian Sardet1,*,{dagger}, Hiroki Nishida2,*, François Prodon1 and Kaichiro Sawada2

1 BioMarCell, UMR 7009, CNRS/UPMC, Station Zoologique, Observatoire Océanologique, Villefranche sur Mer, 06230, France
2 Department of Biological Sciences, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

{dagger} Author for correspondence (e-mail: sardet{at}obs-vlfr.fr)

Accepted 6 August 2003

Localization of maternal mRNAs in the egg cortex is an essential feature of polarity in embryos of Drosophila, Xenopus and ascidians. In ascidians, maternal mRNAs such as macho 1, a determinant of primary muscle-cell fate, belong to a class of postplasmic RNAs that are located along the animal-vegetal gradient in the egg cortex. Between fertilization and cleavage, these postplasmic RNAs relocate in two main phases. They further concentrate and segregate in small posterior blastomeres into a cortical structure, the centrosome-attracting body (CAB), which is responsible for unequal cleavages.

By using high-resolution, fluorescent, in situ hybridization in eggs, zygotes and embryos of Halocynthia roretzi, we showed that macho 1 and HrPEM are localized on a reticulated structure situated within 2 µm of the surface of the unfertilized egg, and within 8 µm of the surface the vegetal region and then posterior region of the zygote. By isolating cortices from eggs and zygotes we demonstrated that this reticulated structure is a network of cortical rough endoplasmic reticulum (cER) that is tethered to the plasma membrane. The postplasmic RNAs macho 1 and HrPEM were located on the cER network and could be detached from it. We also show that macho 1 and HrPEM accumulated in the CAB and the cER network. We propose that these postplasmic RNAs relocalized after fertilization by following the microfilament- and microtubule-driven translocations of the cER network to the poles of the zygote. We also suggest that the RNAs segregate and concentrate in posterior blastomeres through compaction of the cER to form the CAB. A multimedia BioClip `Polarity inside the egg cortex' tells the story and can be downloaded at www.bioclips.com/bioclip.html

Key words: Ascidian embryo, Maternal mRNA, Egg cortex, RNA localization, Endoplasmic reticulum


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